FREE SHIPPING over $40
OVER 9000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Home / Equine Research Bank / Can J Vet Res / Effect of storage time and temperature on the total protein ...
Canadian journal of veterinary research = Revue canadienne de recherche veterinaire2013; 77(4); 293-296;

Effect of storage time and temperature on the total protein concentration and electrophoretic fractions in equine serum.

Abstract: Serum protein electrophoresis (SPE) is a technique that could be considered one of the most useful diagnostic aids available to the clinician. The effect of storage time and temperature on the total proteins and electrophoretic fractions (albumin, α1-, α2-, β1-, β2-, and γ-globulins) was assessed in 24 healthy horses. All samples, collected by jugular vein puncture, were centrifuged and divided into 4 aliquots. The 1st aliquot was analyzed within 3 h from collection (time 0), the 2nd was refrigerated at +4°C for 24 h, the 3rd was refrigerated at +4°C for 48 h, and the last was frozen at -20°C for 48 h. One-way repeated-measures analysis of variance (ANOVA) showed a significant effect (P < 0.05) of the different storage conditions on the concentrations of all the parameters studied and significant variations in the percentages of albumin, α1-globulins, α2-globulins, and γ-globulins. Compared with time 0 the total protein concentration increased significantly after 48 h at -20°C, the albumin percentage decreased after 48 h at -20°C, the α1-globulin percentage increased after 24 h at +4°C, the α2-globulin percentage increased after 48 h at +4°C and at -20°C, and the γ-globulin percentage increased after 48 h at -20°C. The results should help veterinary practitioners handle and store equine serum samples appropriately. Further investigations at different storage times and temperatures could be useful. L’électrophorèse des protéines sériques (SPE) est une technique qui pourrait être considérée comme un des outils diagnostiques les plus utiles au clinicien. L’effet du temps et de la température d’entreposage sur les protéines totales et les fractions électrophorétiques (albumine, α-, α-, β-, β-, et γ-globulines) a été évalué chez 24 chevaux en santé. Tous les échantillons ont été prélevés par ponction de la veine jugulaire, centrifugés et divisés en quatre aliquots. Le premier aliquot a été analysé en dedans de trois heures du moment de la collecte (temps 0), le deuxième a été réfrigéré à 4 °C pour 24 h, le troisième a été réfrigéré à 4 °C pour 48, et le dernier a été congelé à −20 °C pendant 48 h. Une analyse de variance unidirectionnelle sur des mesures répétées (ANOVA) a montré un effet significatif ( < 0,05) des différentes conditions d’entreposage sur les concentrations de tous les paramètres étudiés et des variations significatives dans les pourcentages d’albumine, d’α-globuline, d’α-globuline, et de γ-globuline. Comparativement au temps 0, la concentration de protéines totales a augmenté significativement après 48 h à −20 °C, le pourcentage d’albumine a diminué après 48 h à −20 °C, le pourcentage d’α-globuline a augmenté après 24 h à 4 °C, le pourcentage d’a-globuline a augmenté après 48 h à 4 °C et à −20 °C, et le pourcentage de γ-globuline a augmenté après 48 h à −20 °C. Ces résultats devraient aider les vétérinaires praticiens à manipuler et entreposer de manière appropriée les échantillons de sérum équin. Des études ultérieures sur différents temps et températures d’entreposage seraient utiles.(Traduit par Docteur Serge Messier).
Get Full Text
Publication Date: 2013-10-15 PubMed ID: 24124272PubMed Central: PMC3788661
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article investigates the effect of storage time and temperature on serum protein concentrations, in particular electrophoretic fractions, in healthy horse samples. The results indicate significant variation across different storage conditions and provide recommendations for veterinary practitioners to handle and store equine serum samples appropriately.

Research Methodology

  • The researchers studied the effect of storage time and temperature on the concentration of total proteins and different types of globulins in the serum of 24 healthy horses.
  • The serum was obtained from a jugular vein puncture and was centrifuged and divided into four parts.
  • Each of these parts was stored under different conditions. The first was analyzed within three hours of collection, the second was refrigerated at +4°C for 24 hours, the third refrigerated at +4°C for 48 hours, and the last was frozen at -20°C for 48 hours.

Findings and Analysis

  • An analysis of variance (ANOVA) demonstrated a significant effect of the different storage conditions on the concentrations of all the parameters studied.
  • Significant variations were observed in the percentages of albumin, α1-globulins, α2-globulins, and γ-globulins under different storage conditions compared with time zero.
  • Notably, the total protein concentration increased significantly after 48 hours at -20°C.

Implications

  • This study’s results provide important insight into how the variance in storage time and temperature can impact the protein concentration in horse serum samples. This information is really relevant and potentially beneficial to practitioners in veterinary medicine.
  • Veterinary practitioners are advised to follow these optimal conditions found in the study to handle and store equine serum samples correctly.
  • The authors believe that further investigation into different storage times and temperatures could be beneficial, as this could lead to more refined guidelines for storing equine serum samples.

Cite This Article

APA
Alberghina D, Casella S, Giannetto C, Marafioti S, Piccione G. (2013). Effect of storage time and temperature on the total protein concentration and electrophoretic fractions in equine serum. Can J Vet Res, 77(4), 293-296.

Publication

Canadian journal of veterinary research = Revue canadienne de recherche veterinaire
ISSN: 1928-9022
NlmUniqueID: 8607793
Country: Canada
Language: English
Volume: 77
Issue: 4
Pages: 293-296

Researcher Affiliations

Alberghina, Daniela
  • Dipartimento di Scienze Veterinarie, Università degli Studi di Messina, Polo Universitario dell'Annunziata, 98168 Messina, Italy.
Casella, Stefania
    Giannetto, Claudia
      Marafioti, Simona
        Piccione, Giuseppe

          MeSH Terms

          • Albumins / analysis
          • Animals
          • Blood Chemical Analysis / methods
          • Blood Chemical Analysis / veterinary
          • Electrophoresis, Cellulose Acetate / veterinary
          • Globulins / analysis
          • Horses / blood
          • Reference Values
          • Time Factors

          References

          This article includes 19 references
          1. Colahan PT, Merritt AM, Moore JN, Mayhew G. Equine Medicine Surgery. 5th ed. II. St. Louis, Missouri: Mosby; 1999. pp. 987–1989.
          2. Kaneko JJ. Serum proteins and the dysproteinemias. In: Kaneko JJ, Harvey JW, Bruss ML, editors. Clinical Biochemistry of Domestic Animals. 5th ed. San Diego, California: Academic Press; 1997. pp. 117–137.
          3. Riond B, Wenger-Riggenbach B, Hofmann-Lehmann R, Lutz H. Serum protein concentrations from clinically healthy horses determined by agarose gel electrophoresis.. Vet Clin Pathol 2009 Mar;38(1):73-7.
            pubmed: 19171019doi: 10.1111/j.1939-165x.2008.00100.xgoogle scholar: lookup
          4. Stockham SL. Interpretation of equine serum biochemical profile results.. Vet Clin North Am Equine Pract 1995 Dec;11(3):391-414.
            pubmed: 8925417doi: 10.1016/s0749-0739(17)30307-3google scholar: lookup
          5. Young DS, Bermes EW. Specimen collection and processing: Sources of biological variation. In: Burtis CA, Ashwood ER, editors. Tietz Textbook of Clinical Chemistry. Philadelphia, Pennsylvania: WB Saunders; 1999. pp. 42–72.
          6. Cray C, Rodriguez M, Zaias J, Altman NH. Effects of storage temperature and time on clinical biochemical parameters from rat serum.. J Am Assoc Lab Anim Sci 2009 Mar;48(2):202-4.
            pmc: PMC2679668pubmed: 19383219
          7. Ehsani A, Afshari A, Bahadori H, Mohri M, Seifi HA. Serum constituents analyses in dairy cows: effects of duration and temperature of the storage of clotted blood.. Res Vet Sci 2008 Dec;85(3):473-5.
            pubmed: 18353408doi: 10.1016/j.rvsc.2008.02.004google scholar: lookup
          8. Piccione G, Bertolucci C, Grasso F, Guidice E. Changes in gas composition and acid-base values of venous blood samples stored under different conditions in 4 domestic species.. Vet Clin Pathol 2007 Dec;36(4):358-60.
            pubmed: 18041702doi: 10.1111/j.1939-165x.2007.tb00441.xgoogle scholar: lookup
          9. Casella S, Giannetto C, Fazio F, Giudice E, Piccione G. Assessment of prothrombin time, activated partial thromboplastin time, and fibrinogen concentration on equine plasma samples following different storage conditions.. J Vet Diagn Invest 2009 Sep;21(5):674-8.
            pubmed: 19737763doi: 10.1177/104063870902100512google scholar: lookup
          10. Hawkins MG, Kass PH, Zinkl JG, Tell LA. Comparison of biochemical values in serum and plasma, fresh and frozen plasma, and hemolyzed samples from orange-winged Amazon parrots (Amazona amazonica).. Vet Clin Pathol 2006 Jun;35(2):219-25.
            pubmed: 16783717doi: 10.1111/j.1939-165x.2006.tb00118.xgoogle scholar: lookup
          11. Thoresen SI, Tverdal A, Havre G, Morberg H. Effects of storage time and freezing temperature on clinical chemical parameters from canine serum and heparinized plasma.. Vet Clin Pathol 1995;24(4):129-133.
            pubmed: 12664427doi: 10.1111/j.1939-165x.1995.tb00954.xgoogle scholar: lookup
          12. Tóthová C, Nagy O, Seidel H, Kovacs G. The effect of storage on the protein electrophoretic pattern in bovine serum. Iranian J Vet Sci Technol 2010;2:77–84.
          13. Braun JP, Trumel C, Bézille P. Clinical biochemistry in sheep: A selected review. Small Rumin Res 2010;92:10–18.
          14. . Directive 86/609/EEC of 24 November 1986 on the approximation of laws, regulations and administrative provisions of the Member States regarding the protection of animals used for experimental and other scientific purposes. Off J Eur Communities L 1986;358:1–29.
          15. Martin-Rosset W. L’alimentation des chevaux. Paris, France: Institut national de la recherche agronomique; 1990.
          16. Carapeto V, Barrera M, Cinta Mañe R, Zaragoza MC. Serum α-globulin fraction in horses is related to changes in the acute phase proteins. J Equine Vet Sci 2006;26:120–127.
          17. Cavalcante PH, Silva AC, Sakamoto S, Soto-Blanco B. Serum protein fractions in Brazilian-breed donkeys using agarose gel electrophoresis. Turk J Vet Anim Sci 2012;36:9–12.
          18. Mitruka BM, Rawnsley HM. Clinical Biochemical and Hematological Reference Values in Normal Experimental Animals. New York, Masson; Publishing USA: 1977. Clinical biochemistry; pp. 117–245.
          19. Atmeh RF, Arafa IM, Al-Khateeb M. Albumin aggregates: Hydrodynamic shape and physico-chemical properties. Jordan J Chem 2007;2:169–182.

          Citations

          This article has been cited 3 times.
          1. Junod SL, Saredy J, Yang W. Nuclear Import of Adeno-Associated Viruses Imaged by High-Speed Single-Molecule Microscopy. Viruses 2021 Jan 22;13(2).
            doi: 10.3390/v13020167pubmed: 33499411google scholar: lookup
          2. Hooijberg EH, Miller M, Cray C, Buss P, Steenkamp G, Goddard A. Serum protein electrophoresis in healthy and injured southern white rhinoceros (Ceratotherium simum simum). PLoS One 2018;13(7):e0200347.
            doi: 10.1371/journal.pone.0200347pubmed: 30044807google scholar: lookup
          3. Braceland M, Houston K, Ashby A, Matthews C, Haining H, Rodger H, Eckersall PD. Technical pre-analytical effects on the clinical biochemistry of Atlantic salmon (Salmo salar L.). J Fish Dis 2017 Jan;40(1):29-40.
            doi: 10.1111/jfd.12476pubmed: 27145526google scholar: lookup
          Subscribe to our newsletter
          Join 99,001 horse owners like you who receive our email updates on horse health and nutrition.