Effect of temperature, duration of storage and sampling procedure on ammonia concentration in equine blood plasma.
Abstract: The effect of storage duration at different storage temperatures on the plasma ammonia concentration of equine EDTA whole blood, EDTA plasma and heparin plasma samples was investigated. Further, the effect of jugular vein compression before and during blood sampling on the plasma ammonia values was evaluated. In EDTA whole blood kept at 4 degrees C there was no significant increase of ammonia content after 6 hours of storage, whereas the increase was already significant 3 hours after collection if the EDTA whole blood was kept at 20-22 degrees C. EDTA plasma samples stored at 20-22 degrees C, 4 degrees C and -20 degrees C showed mean ammonia formation rates of 26.8 mumol/l, 6.6 mumol/l and 0.03 mumol/l per day, respectively. There were no differences between ammonia values of EDTA and heparin plasma samples immediately after sampling and following 3 days of storage at 4 degrees C and 3 and 90 days of storage at -20 degrees C. Although significant, the absolute differences between the mean plasma ammonia contents of EDTA blood sampled with and without compression of the jugular vein were small (20.2 +/- 4.1 mumol/l and 23.4 +/- 4.3 mumol/l resp.).
Publication Date: 1993-07-01 PubMed ID: 8399789DOI: 10.1515/cclm.1993.31.7.473Google Scholar: Lookup
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- Comparative Study
- Journal Article
Summary
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The research study examines how storage duration, temperature, and sampling procedures can impact the ammonia concentration in horse blood plasma. It found that temperature and duration significantly affect the ammonia content while the method of blood sampling from the jugular vein results in only modest differences.
Effect of Storage Duration and Temperature
The team studied the influence of storage duration on plasma ammonia concentration in equine EDTA whole blood, EDTA plasma, and heparin plasma samples at various storage temperatures:
- EDTA whole blood stored at 4 degrees Celsius showed no significant increase in ammonia content after six hours. However, if the blood was kept at 20-22 degrees Celsius, the increase in ammonia content became significant three hours post-collection.
- EDTA plasma samples stored at different temperatures had varying ammonia formation rates. Samples stored at room temperature (20-22 degrees Celsius) showed an ammonia formation rate of 26.8 μmol/l per day. Samples stored at 4 degrees Celsius had a lower rate at 6.6 μmol/l per day. The rate dropped significantly for samples stored at -20 degrees Celsius, at only 0.03 μmol/l per day.
Comparison of EDTA and Heparin Plasma Samples
Next, the researchers compared the ammonia values of EDTA and heparin plasma samples:
- The ammonia values did not differ between these types of samples when measured immediately after sampling. There was also no significant difference after storing the samples at 4 degrees Celsius for three days, nor after storing them at -20 degrees Celsius for both three and ninety days.
Impact of Blood Sampling Procedure
The researchers also investigated if the compression of the jugular vein before and during blood sampling exhibited any effect on the plasma ammonia values:
- While there were significant differences in the mean plasma ammonia content between samples collected with and without compression of the jugular vein, the absolute differences were small. The content was 20.2 μmol/l (+/- 4.1) for blood sampled with compression, and 23.4 μmol/l (+/- 4.3) for sampling without compression.
In conclusion, the research emphasizes that the storage conditions, particularly temperature and duration, can greatly affect the ammonia concentration in equine blood samples. Besides, albeit of lesser impact, the method of blood sampling, especially the use of jugular vein compression, can also influence the ammonia content albeit to a smaller extent.
Cite This Article
APA
Lindner A, Bauer S.
(1993).
Effect of temperature, duration of storage and sampling procedure on ammonia concentration in equine blood plasma.
Eur J Clin Chem Clin Biochem, 31(7), 473-476.
https://doi.org/10.1515/cclm.1993.31.7.473 Publication
Researcher Affiliations
- Department of Anatomy, Physiology and Hygiene of Domestic Animals, Faculty of Agronomy, Rheinische Friedrich-Wilhelms-University Bonn, Germany.
MeSH Terms
- Ammonia / blood
- Animals
- Blood Specimen Collection / veterinary
- Edetic Acid
- Heparin
- Horses / blood
- Temperature
- Time Factors
Citations
This article has been cited 2 times.- DeNotta SL, Divers TJ. Clinical Pathology in the Adult Sick Horse: The Gastrointestinal System and Liver.. Vet Clin North Am Equine Pract 2020 Apr;36(1):105-120.
- Qadar Pasha MA, Ram RB, Gupta MD. A rapid method for plasma ammonia estimation using an indigenously purified enzyme.. Indian J Clin Biochem 2000 Aug;15(1):29-35.
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