Effect of time during transport of excised mare ovaries on oocyte recovery rate and quality after in vitro maturation.
Abstract: In the mare only a limited number of oocytes can be successfully collected in vivo, so that when large numbers of oocytes are needed for experimentation, ovaries harvested from slaughtered mares must be used. The resulting temperature changes and time intervals mandated by handling and transport of ovaries from the slaughterhouse to the laboratory adversely affect the rate of oocyte recovery and their quality after IVF and maturation. We chose to study the effect of temperature and time in transit of excised ovaries by evaluating rate of oocyte recovery, nuclear maturation stage reached before, and cleavage rate reached after IVF, following short (1.5 to 4 h) and long (6 to 8 h) storage. Temperatures in the storage container decreased from 37-C to 32 degrees and 27.5 degrees C during the short and long interval, respectively. The cumulus-oocytes complexes (COCs) were classified as having a compact cumulus, completely or partially surrounding the oocyte (compact); those having only a corona radiata surrounding the oocyte (corona); those having a completely or partially expanded cumulus, showing a cellular or sparsely cellular, gelatinous cloud around the oocyte (expanded); and those that were completely denuded of both cumulus and corona cells (denuded). All COCs, except the denuded ones, which were discarded, were matured in vitro for 30 h at 38.5 degrees C in 5% CO2. The recovery rate of oocytes was significantly higher after long vs short storage (48 vs 35%; P < 0.01), but the distribution of the collected COCs into the 4 classes was not affected by the storage time. After in vitro maturation nuclear maturity was not affected by the storage time, but oocytes with intact cytoplasmic membranes were more frequently found after short than after long storage (54 vs 34%; P = 0.07), and fully matured oocytes were more often seen with intact membrane (P < 0.01). Moreover, oocytes with intact membranes in metaphase II (MII) were associated with short storage intervals and the corona COC class, while damaged membranes and incomplete maturation were associated with the long storage and the compact COC class.
Publication Date: 2000-03-29 PubMed ID: 10735117DOI: 10.1016/S0093-691X(99)00169-7Google Scholar: Lookup
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- Journal Article
Summary
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The research focuses on exploring the effects of transportation time of excised mare ovaries on the recovery and quality of oocytes after in vitro maturation. The evidence suggests that a longer transportation time significantly increased oocyte recovery rate but potentially reduced the quality of oocytes.
Research Methodology
- The research involved the collection of ovaries from slaughtered mares. These ovaries were exposed to temperature changes and time intervals during handling and transport from the slaughterhouse to the laboratory.
- The researchers studied the effect of temperature and time in transit on the recovery rate and the nuclear maturation stage and cleavage rate attained before and after in vitro fertilisation (IVF).
- The ovaries were kept in a storage container at two different time intervals: short period (1.5 – 4 hours) and long period (6 – 8 hours). Temperatures in the storage container decreased from the initial 37°C to 32°C and 27.5°C over short and long storage periods, respectively.
- The cumulus-oocytes complexes (COCs) were classified into four categories based on their physical appearance relating to cumulus and corona cells. All COCs, except the completely denuded ones, underwent in vitro maturation for 30 hours.
Research Findings
- The research found that the recovery rate of oocytes was significantly higher in long storage periods compared to short storage periods.
- However, the time in storage did not affect the distribution of COCs into the four classes.
- There was also no significant impact of storage time on nuclear maturity after in vitro maturation.
- The researchers observed that oocytes with intact cytoplasmic membranes were found more frequently after short storage periods compared to long ones.
- Furthermore, fully matured oocytes with intact membranes were more often observed and related to the short storage group. In contrast, damaged membranes and incomplete maturation were associated with long storage periods.
- The research signifies that while a longer storage time increases the oocyte recovery rate, it may also reduce the oocyte quality, affecting the outcomes of IVF procedures.
Cite This Article
APA
Guignot F, Bezard J, Palmer E.
(2000).
Effect of time during transport of excised mare ovaries on oocyte recovery rate and quality after in vitro maturation.
Theriogenology, 52(5), 757-766.
https://doi.org/10.1016/S0093-691X(99)00169-7 Publication
Researcher Affiliations
- Equipe Reproduction Equine, INRA-Haras nationaux Station de Physiologie de la Reproduction, Nouzilly, France.
MeSH Terms
- Animals
- Cell Division
- Cell Membrane / ultrastructure
- Cell Nucleus / physiology
- Cell Nucleus / ultrastructure
- Female
- Horses
- Oocyte Donation / veterinary
- Oocytes / cytology
- Ovarian Follicle / cytology
- Ovary / cytology
Citations
This article has been cited 4 times.- Fujihara M, Shiraishi JI, Onuma M, Ohta Y, Inoue-Murayama M. Cryopreservation Competence of Chicken Oocytes as a Model of Endangered Wild Birds: Effects of Storage Time and Temperature on the Ovarian Follicle Survival.. Animals (Basel) 2022 Jun 2;12(11).
- Shirasawa H, Ono N, Kumazawa Y, Sato W, Sato N, Ihara M, Yaegashi N, Terada Y. Oocyte collection and in vitro maturation after train transportation of human follicular fluid aspirated from resected non-stimulated ovaries of patients with endometrial adenocarcinoma.. Reprod Med Biol 2019 Apr;18(2):180-189.
- Abdoon AS, Abdel-Rahman HA, Shawki SM, Kandil OM, Fathalla SI. Influence of follicle size, methods of retrieval on oocytes yield and morphology in Egyptian Jennies ovaries with special reference to maturation rate in vitro.. Vet Res Commun 2014 Dec;38(4):287-95.
- Abdel-Khalek EA, El-Harairy MA, Shamiah ShM, Khalil WA. Effect of ovary preservation period on recovery rate and categories of dromedary camel oocytes.. Saudi J Biol Sci 2010 Jul;17(3):231-5.
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