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Journal of equine science2013; 24(1); 1-8; doi: 10.1294/jes.24.1

Effect of Treadmill Exercise and Hydrogen-rich Water Intake on Serum Oxidative and Anti-oxidative Metabolites in Serum of Thoroughbred Horses.

Abstract: The present study aimed to clarify changes of oxidative stress and antioxidative functions in treadmill-exercised Thoroughbred horses (n=5, 3 to 7 years old), using recently developed techniques for measurement of serum d-ROMs for oxidative stress, and BAP for antioxidative markers. Also, the effect of nasogastric administration of hydrogen-rich water (HW) or placebo water preceding the treadmill exercise on these parameters was examined. Each horse was subjected to a maximum level of treadmill exercise in which the horses were exhausted at an average speed of 13.2 ± 0.84 m/sec. Blood samples were taken 4 times, immediately before the intake of HW or placebo water at 30 min preceding the treadmill exercise, immediately before the exercise (pre-exercise), immediately after the exercise (post-exercise) and at 30 min following the exercise. In all horses, both d-ROMs and BAP values significantly increased at post-exercise. The increase in d-ROMs tended to be lower in the HW trial, as compared to the placebo trial at pre-exercise. The increase in BAP was considerable at approximately 150% of the pre-exercise values in both the HW and placebo treatment trials. The BAP/d-ROMs ratio was significantly elevated at post-exercise in both treatment trials, while a significant elevation was also observed at pre-exercise in the HW trial. BAP, d-ROM, and the BAP/d-ROM ratio tended to decline at 30 min after the exercise, except BAP and BAP/d-ROMs in the placebo trial. These results demonstrate that the marked elevation of oxidative stress and anitioxidative functions occurred simultaneously in the intensively exercised horses, and suggest a possibility that HW has some antioxidative efficacy.
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Publication Date: 2013-04-16 PubMed ID: 24833996PubMed Central: PMC4013981DOI: 10.1294/jes.24.1Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research studied the effects of treadmill exercise and the intake of hydrogen-rich water on blood levels of oxidative stress and antioxidant measures in Thoroughbred horses. Results suggest that both treadmill exercise and hydrogen-rich water intake may have antioxidant effects, potentially reducing exercise-induced oxidative stress in horses.

Overview of the Study

  • The researchers wanted to examine the changes in oxidative stress and antioxidant functions in Thoroughbred horses (age 3-7, 5 in total) who were put through intense treadmill exercise. They used recent methods to measure serum d-ROMs (indicating oxidative stress) and BAP (showing antioxidative markers).
  • Part of the study also focused on understanding the effect of hydrogen-rich water (HW) or a placebo water given to horses through a tube into the stomach, 30 minutes before the treadmill exercise.
  • The horses were made to run at a maximum speed until they were exhausted, averaging about 13.2 m/sec.
  • Blood samples were taken four times during the study – immediately prior to the intake of HW or placebo, immediately before exercise (pre-exercise), immediately after the exercise (post-exercise), and 30 minutes post-exercise.

Key Findings

  • The study found that both d-ROMs and BAP values increased significantly post-exercise in all the horses. The increase in d-ROMs was slightly lower in the HW trial compared to the placebo during pre-exercise.
  • The increase in BAP was significant, about 150% of the pre-exercise values, in both trials (HW and placebo).
  • The BAP/d-ROMs ratio (an indicator of balance between oxidant and antioxidant systems) rose significantly after the exercise in both situations. This ratio was also seen to rise notably prior to the exercise in the HW trial.
  • All measured parameters tended to decline 30 minutes after the exercise, except BAP and the BAP/d-ROMs ratio in the placebo trial.

Interpretation of Findings

  • The simultaneous marked increase in oxidative stress and antioxidative functions suggests that intense exercise causes a significant physiological response in horses.
  • Hydrogen-rich water may have some antioxidative effect since the increase in oxidative stress markers was lower in the HW trial compared to the placebo during pre-exercise, and the BAP/d-ROMs ratio increased even before the exercise in the HW trial.
  • These findings provide a preliminary indication that HW could potentially be useful in managing exercise-induced oxidative stress in horses, though more research is needed to confirm these effects and understand the underlying mechanisms.

Cite This Article

APA
Tsubone H, Hanafusa M, Endo M, Manabe N, Hiraga A, Ohmura H, Aida H. (2013). Effect of Treadmill Exercise and Hydrogen-rich Water Intake on Serum Oxidative and Anti-oxidative Metabolites in Serum of Thoroughbred Horses. J Equine Sci, 24(1), 1-8. https://doi.org/10.1294/jes.24.1

Publication

Journal of equine science
ISSN: 1340-3516
NlmUniqueID: 9503751
Country: Japan
Language: English
Volume: 24
Issue: 1
Pages: 1-8

Researcher Affiliations

Tsubone, Hirokazu
  • Research Center for Food Safety, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Hanafusa, Masakazu
  • Research Center for Food Safety, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Endo, Maiko
  • Animal Resource Science Center, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 3145 Ago, Ibaraki-Kasama 319-0206, Japan.
Manabe, Noboru
  • Animal Resource Science Center, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 3145 Ago, Ibaraki-Kasama 319-0206, Japan.
Hiraga, Atsushi
  • Hidaka Training Farm, Japan Racing Association, 535-13 Aza-nisi Sha, Urakawa-cho, Hokkaido 057-0171, Japan.
Ohmura, Hajime
  • The Equine Research Institute, Japan Racing Association, 321-4 Tokami-cho, Utsunomiya, Tochigi 320-0856, Japan.
Aida, Hiroko
  • The Equine Research Institute, Japan Racing Association, 321-4 Tokami-cho, Utsunomiya, Tochigi 320-0856, Japan.

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Citations

This article has been cited 16 times.
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