Effect of two virus inactivation methods: electron beam irradiation and binary ethylenimine treatment on determination of reproductive hormones in equine plasma.
Abstract: Ionizing irradiation and binary ethylenimine treatment have previously been shown to be effective for in-vitro inactivation of virus in biological material. In the present study the 2 methods were tested for possible effects on measurable concentrations of reproductive hormones in equine plasma (luteinizing hormone (LH), folliclestimulating hormone (FSH), progesterone (P4), and oestradiol-17 beta (E2)). The inactivation methods were electron beam irradiation with a dose from 11 to 44 kGy or treatment with binary ethylenimine (BEI) in concentrations of 1 and 5 mmol/L. Generally, there was a close correlation (r > 0.8, < 0.001) between pre- and post-treatment hormone levels. Thus, the different phases of the oestrous cycle could be distinguished on the basis of measured hormone concentrations of treated samples. However, both treatments significantly changed hormone concentrations of the plasma samples. For LH, FSH, and E2 the effect of irradiation and BEI treatment was depressive and dose-dependent. For P4 the effect of irradiation was also depressive and dose-dependent. However, the highest dose of BEI resulted in an increase of measured P4 concentration, which may be attributed to changes in the plasma matrix due to the treatment. Although the treatments affected measured hormone concentrations, the close correlation between pre-treatment and post-treatment measurements means that the diagnostic value will remain unchanged.
Publication Date: 1997-01-01 PubMed ID: 9444776PubMed Central: PMC8057043DOI: 10.1186/BF03548485Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research article is about examining the effects of two virus inactivation methods – electron beam irradiation and binary ethylenimine (BEI) treatment – on measurable concentrations of reproductive hormones in equine plasma. Although these treatments do affect hormone concentrations, the study concludes that they do not change their diagnostic value.
Research Aim
The main aim of the research was to evaluate the impact of two widely used virus inactivation methods on the measurable concentrations of reproductive hormones in equine plasma. The inactivation methods being tested were electron beam irradiation and binary ethylenimine (BEI) treatment, and the hormones tested included luteinizing hormone (LH), follicle-stimulating hormone (FSH), progesterone (P4), and oestradiol-17 beta (E2).
Methodology
- Electron beam irradiation was administered in doses varying from 11 to 44 kGy
- BEI treatment was given in two different concentrations – 1 mmol/L and 5 mmol/L
Results
- A close correlation (r > 0.8, < 0.001) was observed between pre- and post-treatment hormone levels. This indicates that even after the treatment, the hormone concentrations could still provide clear indications of the different phases of the oestrous cycle.
- However, both treatments did bring about significant changes in hormone concentrations in the plasma samples. These changes were depressive and dose-dependent for LH, FSH, and E2, under both irradiation and BEI treatment. The same depressive and dose-dependent effect was noticed for P4 under irradiation.
- Interestingly though, the highest dosage of BEI resulted in an increase in measured P4 concentration. The researchers have attributed this unexpected result to changes in the plasma matrix caused by BEI treatment.
Conclusions
In spite of the changes recognized in hormone concentrations after treatment with both irradiation and BEI, the researchers concluded that the diagnostic value of these hormones remains unchanged. This is due to the close correlation between pre-treatment and post-treatment hormone measurements, underlining the usefulness and efficiency of these inactivation methods.
Cite This Article
APA
Kyvsgaard NC, Høier R, Brück I, Nansen P.
(1997).
Effect of two virus inactivation methods: electron beam irradiation and binary ethylenimine treatment on determination of reproductive hormones in equine plasma.
Acta Vet Scand, 38(3), 225-233.
https://doi.org/10.1186/BF03548485 Publication
Researcher Affiliations
- Danish Centre for Experimental Parasitology, Royal Veterinary and Agricultural University, Frederiksberg, Denmark. nck@kvl.dk
MeSH Terms
- Animals
- Aziridines / pharmacology
- Dose-Response Relationship, Radiation
- Estradiol / blood
- Female
- Follicle Stimulating Hormone / blood
- Horses / blood
- Horses / physiology
- Luteinizing Hormone / blood
- Progesterone / blood
- Viruses / drug effects
- Viruses / growth & development
- Viruses / radiation effects
References
This article includes 23 references
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Citations
This article has been cited 1 times.- Habib M, Hussain I, Irshad H, Yang ZZ, Shuai JB, Chen N. Immunogenicity of formaldehyde and binary ethylenimine inactivated infectious bursal disease virus in broiler chicks. J Zhejiang Univ Sci B 2006 Aug;7(8):660-4.
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