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Journal of equine science2016; 27(4); 149-156; doi: 10.1294/jes.27.149

Effects of a single use of the GnRH analog buserelin on the induction of ovulation and endocrine profiles in heavy draft mares.

Abstract: We observed structural changes in the follicles and uterus of heavy draft mares during estrus and examined the effect of a single injection of the gonadotropin-releasing hormone analog buserelin on ovulation and endocrine profiles. Twenty-two heavy draft mares were divided into a buserelin-treated group (n=8) and a control group (n=14). Mares were given an intramuscular injection of 40 µg buserelin when they presented signs of estrus to a teaser stallion, had ≥45 mm diameter follicles, and presented decreased uterine edema compared with the previous examination. The follicles and uterus were monitored using transrectal ultrasound imaging and measurement of blood levels of luteinizing hormone (LH), follicle-stimulating hormone (FSH), progesterone, and estradiol-17β. The ovulation rates within 48 hr was significantly higher in the treated group (100%, 8/8) than in the control group (57.1%, 8/14; P=0.051). The mean ± SEM time before confirmation of ovulation was 29 ± 9 hr for the treated group and 59 ± 7 hr for the control group. There were no significant differences in mating frequency, double ovulation rate, or fertility rate between the two groups. One to two days after administering buserelin, LH and FSH temporarily increased, and in the control group, LH was high during ovulation, whereas FSH temporarily increased with the growth of the follicle. These results indicate that a single injection of 40 µg buserelin when follicles are at least 45 mm in diameter and uterine edema is decreased is effective for inducing ovulation.
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Publication Date: 2016-12-15 PubMed ID: 27974874PubMed Central: PMC5155133DOI: 10.1294/jes.27.149Google Scholar: Lookup
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Summary

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The research investigates the impact of administering a single injection of the hormone buserelin on the ovulation process and endocrine interactions in heavy draft mares. The study found that the injection significantly increased ovulation rate in the treated mares and speeded up the confirmation of ovulation while maintaining similar fertility rates to untreated mares.

Research Methods and Participants

  • Twenty-two heavy draft mares were involved in this experiment. These were divided into two groups: those treated with buserelin (8 mares), and a control group that received no treatment (14 mares).
  • The treated mares received an intramuscular injection of 40 µg buserelin when they showed signs of being in estrus (heat), and had follicles at least 45 mm in diameter and diminished uterine swelling compared to previous examination.

Data Collection

  • The study used transrectal ultrasound imaging to monitor the mares’ uterus and follicles. In addition, blood levels of luteinizing hormone (LH), follicle-stimulating hormone (FSH), progesterone, and estradiol-17β were measured to assess the endocrine profiles of the mares.

Results and Findings

  • The study found that the rate of ovulation within 48 hours was significantly higher in the buserelin-treated group (100%) compared with the control group (57.1%).
  • On average, ovulation confirmation was achieved faster in the treated group (29 hours) than the control group (59 hours).
  • Despite the altered ovulation rate, there were no significant differences between the two groups in terms of mating frequency, double ovulation rate, or fertility rate.
  • Blood hormone levels showed that 1-2 days after administering buserelin, LH and FSH levels temporarily increased. In the control group, LH was found to be high during ovulation, while FSH levels rose with follicle growth.

Conclusions

  • The findings suggest that a single injection of 40 µg buserelin is effective in inducing ovulation when administered at the right time. This is determined by the size of follicles (being at least 45 mm in diameter) and a decrease in uterine edema.

Cite This Article

APA
Miki W, Oniyama H, Takeda N, Kimura Y, Haneda S, Matsui M, Taya K, Nambo Y. (2016). Effects of a single use of the GnRH analog buserelin on the induction of ovulation and endocrine profiles in heavy draft mares. J Equine Sci, 27(4), 149-156. https://doi.org/10.1294/jes.27.149

Publication

Journal of equine science
ISSN: 1340-3516
NlmUniqueID: 9503751
Country: Japan
Language: English
Volume: 27
Issue: 4
Pages: 149-156

Researcher Affiliations

Miki, Wataru
  • The United Graduate School of Veterinary Sciences, Gifu University, Gifu 501-1193, Japan; Federation of Hokkaido Agricultural Mutual Aid Associations, Hokkaido 069-0806, Japan.
Oniyama, Hiroyuki
  • Tokachi Agricultural Mutual Aid Association, Hokkaido 089-1182, Japan.
Takeda, Naomasa
  • Department of Clinical Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido 080-8555, Japan.
Kimura, Yuki
  • The United Graduate School of Veterinary Sciences, Gifu University, Gifu 501-1193, Japan.
Haneda, Shingo
  • Department of Clinical Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido 080-8555, Japan.
Matsui, Motozumi
  • Department of Clinical Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido 080-8555, Japan.
Taya, Kazuyoshi
  • Tokyo University of Agriculture and Technology, Tokyo 183-8538, Japan.
Nambo, Yasuo
  • Department of Clinical Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido 080-8555, Japan.

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Citations

This article has been cited 3 times.
  1. Khan IU, Khairullah AR, Khan AY, Rehman AU, Mustofa I. Strategic approaches to improve equine breeding and stud farm outcomes. Vet World 2025 Feb;18(2):311-328.
    doi: 10.14202/vetworld.2025.311-328pubmed: 40182817google scholar: lookup
  2. Bukhari SSUH, Urooj S. Retinol and cholecalciferol affect buserelin-induced estrous in anestrous mares. Trop Anim Health Prod 2025 Mar 17;57(3):124.
    doi: 10.1007/s11250-025-04369-9pubmed: 40095200google scholar: lookup
  3. Pemayun TGO, Mustofa I, Mahaputra L, Hermadi HA, Wijaya NMR, Mulyati S, Utama S, Restiadi TI, Rimayanti R. Fertility restoration of racing mare with persistent corpus luteum. Vet World 2021 Sep;14(9):2356-2361.
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