Effects of age and R848 stimulation on expression of Toll-like receptor 8 mRNA by foal neutrophils.
Abstract: The innate immune system plays a critical role in protecting neonates against infections early in life and Toll-like receptors (TLRs) are key components of innate immune recognition of pathogens. This study examined the effects of age and stimulation with a TLR 7/8 agonist (R848) on TLR8 mRNA expression by foal neutrophils during the first month of life. We also examined the effects of R848 stimulation on mRNA expression of interleukin (IL)-6 and IL-8 at 1 and 14 days of life. We observed that TLR8 mRNA was constitutively expressed (P<0.05) at all ages examined, and its expression did not change upon stimulation with R848. Stimulation with R848 resulted in significantly increased (P<0.05) expression of IL-6 and IL-8 mRNA at both 1 and 14 days of life. Our results demonstrate that foal neutrophils express constitutive levels of TLR8 mRNA. The increased expression of IL-6 and IL-8, both downstream products of the TLR 7/8 signaling pathway, following stimulation with R848 suggests that additional experiments to confirm the signaling pathway by which R848 stimulates foal neutrophils, and to investigate its ability to stimulate functional responses of foal neutrophils, are merited.
Copyright © 2012 Elsevier B.V. All rights reserved.
Publication Date: 2012-08-04 PubMed ID: 22901428DOI: 10.1016/j.vetimm.2012.07.012Google Scholar: Lookup
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- Journal Article
- Research Support
- N.I.H.
- Extramural
- Research Support
- Non-U.S. Gov't
Summary
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The research in this article investigates the effects of age and stimulation on the immune response of young foals, particularly focusing on a key component of the immune system: Toll-like receptor 8. The results suggest that the immune system of a foal may respond differently to certain stimulations at different ages.
Objective of the Research
- The main aim of this research was to evaluate the impact of age and a specific stimulant – known as R848 – on the expression of Toll-like receptor 8 (TLR8) mRNA in foal neutrophils. The researchers also wanted to observe the effects of R848 stimulations on mRNA expression of Interleukin (IL)-6 and IL-8 at the ages of 1 and 14 days.
Methodology
- Toll-like receptor 8 mRNA expression by foal neutrophils was evaluated and compared at different ages during the foal’s first month of life.
- The researchers evaluated the effects of stimulation by R848, a TLR 7/8 agonist, on the expression of the TLR8 mRNA.
- The researchers also studied the impact of R848 stimulation on expression of IL-6 and IL-8, both of which are downstream products of the TLR 7/8 signaling pathway, at days 1 and 14 of a foal’s life.
Key Findings
- The researchers found that TLR8 mRNA was constantly present, and its level of expression did not change with R848 stimulation.
- It was observed that stimulation with R848 did, however, significantly increase expression of IL-6 and IL-8 mRNA at days 1 and 14.
Conclusions and Recommendations for Further Research
- This study demonstrates that foal neutrophils constantly express TLR8 mRNA. The increased expression of IL-6 and IL-8 following stimulation with R848 indicates that the TLR 7/8 signaling pathway could be involved, suggesting that additional research to confirm this and to explore its ability to stimulate functional responses of foal neutrophils is warranted.
Cite This Article
APA
Harrington JR, Wilkerson CP, Brake CN, Cohen ND.
(2012).
Effects of age and R848 stimulation on expression of Toll-like receptor 8 mRNA by foal neutrophils.
Vet Immunol Immunopathol, 150(1-2), 10-18.
https://doi.org/10.1016/j.vetimm.2012.07.012 Publication
Researcher Affiliations
- Department of Large Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX 77843, USA. Jharrington@cvm.tamu.edu
MeSH Terms
- Age Factors
- Animals
- Animals, Newborn
- Gene Expression Regulation / drug effects
- Horses / genetics
- Horses / immunology
- Imidazoles / pharmacology
- Immunity, Innate / drug effects
- Immunity, Innate / immunology
- Interleukin-6 / genetics
- Interleukin-6 / immunology
- Interleukin-8 / genetics
- Interleukin-8 / immunology
- Linear Models
- Male
- Neutrophils / drug effects
- Neutrophils / immunology
- RNA, Messenger / biosynthesis
- RNA, Messenger / chemistry
- RNA, Messenger / genetics
- Reverse Transcriptase Polymerase Chain Reaction / veterinary
- Signal Transduction
- Toll-Like Receptor 8 / agonists
- Toll-Like Receptor 8 / genetics
- Toll-Like Receptor 8 / immunology
Citations
This article has been cited 1 times.- Migdał A, Migdał Ł, Oczkowicz M, Okólski A, Chełmońska-Soyta A. Influence of Age and Immunostimulation on the Level of Toll-Like Receptor Gene (TLR3, 4, and 7) Expression in Foals.. Animals (Basel) 2020 Oct 26;10(11).
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