Effects of amikacin sulfate on the motility of stallion and bull spermatozoa at different temperatures and intervals of storage.
Abstract: Because microfloral content of stallion semen tends to be high, and strains may be resistant to commonly used antibiotics, amikacin was tested with stallion semen and compared with bull semen. Nine ejaculates to stallion semen were incubated at 37 C in egg yolk-tris extender for 0, 2, 4, 6, 8 and 10 h in the presence of amikacin concentrations of 0, 50, 100, 250, 500, 1,000 and 10,000 microgram/ml, with penicillin and penicillin-streptomycin as controls. Averaged over all incubations, spermatozoal motility was 44, 48, 49, 46, 45, 45 and 19%, for increasing concentrations of amikacin, compared with 52 and 47% for penicillin and penicillin-streptomycin controls. The 10,000 microgram/ml concentration of amikacin was the only treatment that suppressed sperm motility (P less than .01). Amikacin (0, 50, 100, 250, 500, 1,000 2,500, 5,000 and 10,000 microgram/ml) and 1,000 IU of penicillin G plus 1,000 microgram of streptomycin/ml or 10,000 IU of penicillin G/ml were added to nine ejaculates of bull semen stored at 4 C in egg yolk-tris extender, and evaluated after 0, 1, 3, 5 and 7 d. The percentage of motile spermatozoa, with increasing levels of amikacin, was 66, 67, 66, 64, 67, 68, 74, 68 and 53%, respectively. Amikacin, at 2,500 microgram/ml, resulted in the highest (P less than .01) motility compared to the other levels of antibiotics after 7 d storage. Both 10,000 microgram of amikacin and 10,000 IU of penicillin G/ml depressed (P less than .01) the mean percentage of motile bull spermatozoa. These studies demonstrate that high concentrations of amikacin can be added to stallion and bull semen without depressing motility of spermatozoa.
Publication Date: 1982-06-01 PubMed ID: 7107529DOI: 10.2527/jas1982.5461105xGoogle Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research study investigates the effects of the antibiotic amikacin sulfate on the motility or movement of stallion and bull sperm at various temperatures and storage durations. The results indicate that high concentrations of this antibiotic do not significantly reduce sperm motility, except for an extremely high concentration in stallion sperm.
About the Experiment
- The experiment was conducted due to the high microfloral content seen in stallion semen, where many strains can be resistant to conventional antibiotics. Amikacin was tested with both stallion semen and bull semen for comparison.
- Nine ejaculates of stallion semen were incubated at 37 Celsius in egg yolk-tris extender for 0, 2, 4, 6, 8 and 10 hours. The semen was exposed to various concentrations of amikacin, ranging from 0 to 10,000 micrograms/ml, with penicillin and penicillin-streptomycin serving as controls.
- Nine ejaculates of bull semen were similarly tested, but stored at 4 Celsius in egg yolk-tris extender and evaluated after 0, 1, 3, 5, and 7 days.
Findings of the Research
- For the stallion semen, average sperm motility over all incubations ranged from 44% to 49% at increasing concentrations of amikacin, compared with 52% for penicillin and 47% for penicillin-streptomycin controls.
- The exception was the 10,000 micrograms/ml concentration of amikacin, which significantly suppressed sperm motility.
- Bull semen showed a percentage of motile spermatozoa, with increasing levels of amikacin, between 66% and 74%.
- Interestingly, amikacin concentration of 2,500 micrograms/ml resulted in the highest motility after 7 days of storage for bull semen.
- However, both a concentration of 10,000 micrograms of amikacin and 10,000 IU of penicillin G/ml resulted in significant decrease in sperm motility for the bull semen.
Conclusion of the Study
- The results suggest that amikacin can potentially be used as an alternative to commonly used antibiotics for treating stallion semen with high microfloral content due to its minimal effect on sperm motility at reasonable concentrations.
- The study could influence how semen from these species is stored and treated, potentially improving their long-term viability and fertility prospects.
Cite This Article
APA
Arriola J, Foote RH.
(1982).
Effects of amikacin sulfate on the motility of stallion and bull spermatozoa at different temperatures and intervals of storage.
J Anim Sci, 54(6), 1105-1110.
https://doi.org/10.2527/jas1982.5461105x Publication
Researcher Affiliations
MeSH Terms
- Amikacin / pharmacology
- Animals
- Cattle
- Horses
- Kanamycin / analogs & derivatives
- Male
- Semen Preservation / veterinary
- Sperm Motility / drug effects
- Temperature
- Time Factors
Citations
This article has been cited 3 times.- Wang YH, Chen YH, Shen WH. Amikacin Suppresses Human Breast Cancer Cell MDA-MB-231 Migration and Invasion.. Toxics 2020 Nov 20;8(4).
- Santos CS, Silva AR. Current and alternative trends in antibacterial agents used in mammalian semen technology.. Anim Reprod 2020 Mar 17;17(1):e20190111.
- Al-Kass Z, Spergser J, Aurich C, Kuhl J, Schmidt K, Johannisson A, Morrell JM. Sperm Quality during Storage Is Not Affected by the Presence of Antibiotics in EquiPlus Semen Extender but Is Improved by Single Layer Centrifugation.. Antibiotics (Basel) 2017 Dec 21;7(1).
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