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Home / Equine Research Bank / Am J Vet Res / Effects of autologous stromal cells and cytokines on differe...
American journal of veterinary research2017; 78(10); 1215-1228; doi: 10.2460/ajvr.78.10.1215

Effects of autologous stromal cells and cytokines on differentiation of equine bone marrow-derived progenitor cells.

Abstract: OBJECTIVE To develop an in vitro system for differentiation of equine B cells from bone marrow hematopoietic progenitor cells on the basis of protocols for other species. SAMPLE Bone marrow aspirates aseptically obtained from 12 research horses. PROCEDURES Equine bone marrow CD34 cells were sorted by use of magnetic beads and cultured in medium supplemented with cytokines (recombinant human interleukin-7, equine interleukin-7, stem cell factor, and Fms-like tyrosine kinase-3), murine OP9 stromal cell preconditioned medium, and equine fetal bone marrow mesenchymal stromal cell preconditioned medium. Cells in culture were characterized by use of flow cytometry, immunocytofluorescence microscopy, and quantitative reverse-transcriptase PCR assay. RESULTS For these culture conditions, bone marrow-derived equine CD34 cells differentiated into CD19IgM B cells that expressed the signature transcription factors early B-cell factor and transcription factor 3. These conditions also supported the concomitant development of autologous stromal cells, and their presence was supportive of B-cell development. CONCLUSIONS AND CLINICAL RELEVANCE Equine B cells were generated from bone marrow aspirates by use of supportive culture conditions. In vitro generation of equine autologous B cells should be of use in studies on regulation of cell differentiation and therapeutic transplantation.
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Publication Date: 2017-09-26 PubMed ID: 28945121PubMed Central: PMC5656228DOI: 10.2460/ajvr.78.10.1215Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research paper talks about the progress made in developing an in vitro system for differentiation of equine (horse) B cells from bone marrow hematopoietic progenitor cells. This was achieved by using certain supplementary components (cytokines and stromal cells), and the results showed successful creation of specific B cells. This process could be helpful in investigating cell differentiation regulation and therapeutic transplantation.

Objective

  • The research aimed to build an in vitro method where the B cells in horses can be differentiated from bone marrow hematopoietic progenitor cells. These methods are built based on protocols used for different species.

Sample

  • The researchers used bone marrow aspirates, which were aseptically obtained from 12 research horses.

Procedures

  • Sorted CD34 cells from equine bone marrow were cultured in a specialized medium, supplemented with certain cytokines (recombinant human interleukin-7, equine interleukin-7, stem cell factor, and Fms-like tyrosine kinase-3).
  • The conditioned medium from murine OP9 stromal cell and equine fetal bone marrow mesenchymal stromal cell was also added to these cultures.
  • Characterization of the cells in the culture was carried out with flow cytometry, immunocytofluorescence microscopy, and quantitative reverse-transcriptase PCR assay.

Results

  • The bone marrow-derived equine CD34 cells differentiated into CD19IgM B cells under the provided culture conditions. These B cells indicated the expression of signature transcription factors early B-cell factor and transcription factor 3.
  • The presence of autologous stromal cells was noticed alongside, and they were supportive of B-cell development.

Conclusions and Clinical Relevance

  • Equine B cells were successfully generated from bone marrow aspirates through the aid of supportive culture conditions.
  • The generation of these autologous B cells in lab conditions would be useful for studies related to regulation of cell differentiation and for therapeutic transplantation purposes.

Cite This Article

APA
Schwab UE, Tallmadge RL, Matychak MB, Felippe MJB. (2017). Effects of autologous stromal cells and cytokines on differentiation of equine bone marrow-derived progenitor cells. Am J Vet Res, 78(10), 1215-1228. https://doi.org/10.2460/ajvr.78.10.1215

Publication

American journal of veterinary research
ISSN: 1943-5681
NlmUniqueID: 0375011
Country: United States
Language: English
Volume: 78
Issue: 10
Pages: 1215-1228

Researcher Affiliations

Schwab, Ute E
    Tallmadge, Rebecca L
      Matychak, Mary Beth
        Felippe, M Julia B

          MeSH Terms

          • Animals
          • Bone Marrow Cells / cytology
          • Bone Marrow Cells / drug effects
          • Cell Differentiation
          • Cells, Cultured
          • Cytokines / pharmacology
          • Female
          • Flow Cytometry
          • Horses
          • Male
          • Mice
          • Stromal Cells / cytology

          Grant Funding

          • DP2 OD007216 / NIH HHS

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