Effects of blood contamination on peritoneal D-dimer concentration in horses with colic.
Abstract: Peritoneal D-Dimer concentration can be determined to assess peritoneal fibrinolysis activity in horses with gastrointestinal disorders. However, blood contamination of peritoneal fluid may occur during collection and could alter peritoneal D-Dimer concentration. Objective: Blood contamination in peritoneal fluid does not affect interpretation of peritoneal D-Dimer concentration in horses with colic. Methods: Thirty-four horses with colic and 4 healthy horses. Methods: Peritoneal fluid and blood samples were simultaneously collected upon admission. Then, peritoneal fluid was serially contaminated with the horse's own blood; final contaminations corresponded to 1, 5, 10, and 20% of blood in peritoneal fluid. D-Dimer concentration was determined in blood, peritoneal fluid, and contaminated peritoneal fluid samples. Data were analyzed using a longitudinal linear model and a generalized estimating equations analysis to assess the quantitative and qualitative variations of the effect of blood contamination on peritoneal D-Dimer concentration. Results: Peritoneal D-Dimer concentration was only quantitatively affected when peritoneal fluid was contaminated at 20% of blood. However, when using increasing cut-off values of peritoneal D-Dimer concentration (100, 2,000, 8,000, and 16,000 ng/mL), this effect disappeared at the highest cut-off values (8,000 and 16,000 ng/mL). When peritoneal fluid contamination was grouped as "minimally contaminated" ( or =5% of blood), no significant differences on D-Dimer concentration between both groups at each cut-off value were observed. Conclusions: Although quantitative results of peritoneal D-Dimer concentration could be affected by high levels of blood contamination (> or =20%), interpretation of increased peritoneal fibrinolytic activity was not significantly affected.
Publication Date: 2009-09-16 PubMed ID: 19761478DOI: 10.1111/j.1939-1676.2009.0381.xGoogle Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research examined whether the contamination of peritoneal fluid with blood affects the interpretation of peritoneal D-Dimer concentration in horses with colic, concluding that even though high levels of blood contamination can theoretically influence the quantitative results, the interpretation of increased peritoneal fibrinolytic activity would not be significantly affected.
Research Details
- The researchers examined how blood contamination could affect the concentration of D-Dimer, a protein fragment that is present after a blood clot dissolves, in the peritoneal fluid of horses suffering from colic.
- The study was conducted on 34 horses with colic and 4 healthy horses.
Procedures
- Upon admission, blood and peritoneal fluid samples were collected from the horses.
- The peritoneal fluid was then progressively contaminated with the horse’s own blood upto 1%, 5%, 10%, and 20% of the fluid sample.
- The D-Dimer concentration was measured in these peritoneal fluid samples, as well as in the blood samples.
- A longitudinal linear model and a generalized estimating equations analysis were used to evaluate changes in D-Dimer concentration due to blood contamination.
Results
- It was found that only when the peritoneal fluid was contaminated with 20% of blood, the D-Dimer concentration was quantitatively affected.
- However, this effect was not observed when the D-Dimer concentration exceeded 8,000 and 16,000 ng/mL.
- Upon grouping the contaminated samples as ‘minimally contaminated’ (less than or equal to 1% blood) and ‘highly contaminated’ (5% or more blood), there was no significant difference in the D-Dimer concentration at various cut-off values.
Conclusions
- The study concluded that even though high levels of blood contamination (> or =20%) could potentially affect the measured values of D-Dimer concentration, it would not significantly affect the interpretation of increased peritoneal fibrinolytic activity.
Cite This Article
APA
Delgado MA, Monreal L, Armengou L, Segura D, Ríos J.
(2009).
Effects of blood contamination on peritoneal D-dimer concentration in horses with colic.
J Vet Intern Med, 23(6), 1232-1238.
https://doi.org/10.1111/j.1939-1676.2009.0381.x Publication
Researcher Affiliations
- Departament de Medicina i Cirurgia Animals, Servei de Medicina Interna Equina, Facultat de Veterinària, Universitat Autònoma de Barcelona, Barcelona, Spain.
MeSH Terms
- Animals
- Ascitic Fluid / chemistry
- Biomarkers
- Blood
- Colic / diagnosis
- Colic / veterinary
- Female
- Fibrin Fibrinogen Degradation Products / analysis
- Horse Diseases / blood
- Horse Diseases / diagnosis
- Horses
- Male
- Specimen Handling / veterinary
Citations
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