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Home / Equine Research Bank / Acta Vet Scand / Effects of cashew gum and nanoparticles on cooled stallion s...
Acta veterinaria Scandinavica2020; 62(1); 31; doi: 10.1186/s13028-020-00530-6

Effects of cashew gum and nanoparticles on cooled stallion semen.

Abstract: Cryopreservation of stallion spermatozoa tends to cause plasma membrane damage due to the low ratio of cholesterol to phospholipids. Gums have been suggested as an alternative cryoprotectant to glycerol for stallion spermatozoa. Therefore, the present experiment was designed to verify whether the effect of addition of cashew gum (CG), or nanoparticles (NP) containing CG, to the extender before cooling on sperm quality in stallion semen. Ejaculates from 6 stallions were extended and split between six treatment groups (control, a-tocopherol [TOC], CG1, CG0.5, NP1 and NP0.5), stored in cryotubes at 4 °C. Results: Aliquots were analysed by computer-assisted sperm motility analysis on the day of collection, and after 24 h and 48 h of cold storage. After 48 h, the total motility with NP1 (78.53 + 6.31%) was similar to control 85.79 + 6.31% at 0 h. The same pattern was observed for progressive motility. Membrane integrity assessed by flow cytometer was similar between control, TOC and G1 at all storage times. The DNA fragmentation in the control group increased at all time points, whereas chromatin integrity was maintained after 24 h in TOC and NP0.5 compared to 0 h. There was no increase in the proportion of live spermatozoa producing hydrogen peroxide, but there was a tendency for an increased proportion of spermatozoa in the live superoxide category in CG1 after 24 h cooled storage. Conclusions: The addition of CG or CG-derived NP to extender for stallion semen was not harmful to the sperm cells.
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Publication Date: 2020-06-18 PubMed ID: 32552825PubMed Central: PMC7302125DOI: 10.1186/s13028-020-00530-6Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigates the impact of adding cashew gum and nanoparticles on the sperm quality of cooled stallion semen. The study found no negative effects when cashew gum or cashew gum-derived nanoparticles are added to the semen.

Objective and Methodology:

  • The study aimed at determining the effect of adding cashew gum, or nanoparticles containing cashew gum, to a liquid substance that preserves, or extends, semen before it is cooled. This was undertaken with the objective of improving sperm quality in stallion semen.
  • The semen of 6 stallions was extended and divided among six treatment groups, each group receiving a different treatment. These were a control group, a-tocopherol, two groups with different concentrations of cashew gum (CG1 and CG0.5), and two groups with different concentrations of nanoparticles containing cashew gum (NP1 and NP0.5).
  • The extended semen was stored in cryotubes at 4°C. Aliquots of the extended semen were analysed at the time of collection and after 24 hours and 48 hours of cold storage.

Results:

  • The study found that total motility with NP1 (one concentration of cashew gum nanoparticles) after 48 hours was similar to that of the control group on collection day. This was also true for progressive motility.
  • When assessing the integrity of the plasma membrane through a flow cytometer, the study found similar results between the control group, a-tocopherol, and one concentration of cashew gum (G1) across all storage times.
  • DNA fragmentation increased in the control group with time, but the chromatin integrity was maintained after 24 hours in a-tocopherol and with one concentration of cashew gum nanoparticles (NP0.5) compared to when it was collected.
  • The study found no increase in the proportion of live sperm cells producing hydrogen peroxide, a potential indicator of cell damage. However, there was a slight increase in the category of live sperm cells in the CG1 group after 24 hours cooled storage.

Conclusion:

  • The research concluded that the addition of either cashew gum or nanoparticles containing cashew gum in the extender for stallion semen does not harm the sperm cells. This suggests that these compounds could potentially be used as substances for preserving stallion semen.

Cite This Article

APA
Loureiro KC, Lima-Verde IB, Johannisson A, Ntallaris T, Jager A, Štěpánek P, da Costa Mendonça M, Severino P, Morrell JM. (2020). Effects of cashew gum and nanoparticles on cooled stallion semen. Acta Vet Scand, 62(1), 31. https://doi.org/10.1186/s13028-020-00530-6

Publication

Acta veterinaria Scandinavica
ISSN: 1751-0147
NlmUniqueID: 0370400
Country: England
Language: English
Volume: 62
Issue: 1
Pages: 31
PII: 31

Researcher Affiliations

Loureiro, Kahynna Cavalcante
  • Laboratory of Nanotechnology and Nanomedicine (LNMED), Institute of Technology and Research (ITP), Av. Murilo Dantas 300, Aracaju, 49010-390, Brazil.
  • Postgraduate Program in Industrial Biotechnology (PBI), Tiradentes University (UNIT), Av. Murilo Dantas 300, Aracaju, 49032-490, Brazil.
  • Department of Supramolecular Polymer Systems, Institute of Macromolecular Chemistry, Heyrovského námestí 2, 162 06, Prague 6, Czech Republic.
Lima-Verde, Isabel Bezerra
  • Department of Clinical Sciences, Swedish University of Agricultural Sciences, Box 7054, 75007, Uppsala, Sweden. isabel_limaverde@yahoo.com.br.
Johannisson, Anders
  • Department of Clinical Sciences, Swedish University of Agricultural Sciences, Box 7054, 75007, Uppsala, Sweden.
Ntallaris, Theodoros
  • Department of Clinical Sciences, Swedish University of Agricultural Sciences, Box 7054, 75007, Uppsala, Sweden.
Jager, Alessandro
  • Department of Supramolecular Polymer Systems, Institute of Macromolecular Chemistry, Heyrovského námestí 2, 162 06, Prague 6, Czech Republic.
Štěpánek, Petr
  • Department of Supramolecular Polymer Systems, Institute of Macromolecular Chemistry, Heyrovského námestí 2, 162 06, Prague 6, Czech Republic.
da Costa Mendonça, Marcelo
  • Laboratory of Nanotechnology and Nanomedicine (LNMED), Institute of Technology and Research (ITP), Av. Murilo Dantas 300, Aracaju, 49010-390, Brazil.
  • Postgraduate Program in Industrial Biotechnology (PBI), Tiradentes University (UNIT), Av. Murilo Dantas 300, Aracaju, 49032-490, Brazil.
Severino, Patrícia
  • Laboratory of Nanotechnology and Nanomedicine (LNMED), Institute of Technology and Research (ITP), Av. Murilo Dantas 300, Aracaju, 49010-390, Brazil.
  • Postgraduate Program in Industrial Biotechnology (PBI), Tiradentes University (UNIT), Av. Murilo Dantas 300, Aracaju, 49032-490, Brazil.
Morrell, Jane M
  • Department of Clinical Sciences, Swedish University of Agricultural Sciences, Box 7054, 75007, Uppsala, Sweden.

MeSH Terms

  • Anacardium / chemistry
  • Animals
  • Cryoprotective Agents / chemistry
  • Cryoprotective Agents / pharmacology
  • Gingiva / chemistry
  • Horses / physiology
  • Male
  • Nanoparticles / chemistry
  • Semen / physiology
  • Semen Preservation / instrumentation
  • Semen Preservation / veterinary

Grant Funding

  • H-14-47-008 / SSH

Conflict of Interest Statement

The authors declare that they have no competing interests.

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Citations

This article has been cited 2 times.
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