Effects of chlorhexidine gluconate and chlorous acid-chlorine dioxide on equine fibroblasts and Staphylococcus aureus.
Abstract: Equine fibroblasts and Staphylococcus aureus were exposed for 30 minutes to six dilutions of chlorhexidine gluconate, a chlorous acid-chlorine dioxide irrigation solution, a chlorous acid-chlorine dioxide disinfectant, and phosphate buffered saline controls. Cell viability was determined by trypsinizing the cells, staining them with trypan blue, and counting cells that did not take the stain. All fibroblasts were killed when exposed to 1.0% and 0.5% chlorhexidine. The survival rate of fibroblasts increased linearly with decreasing concentrations of chlorhexidine gluconate, with a peak survival of 50% at 0.005% chlorhexidine. The chlorous acid-chlorine dioxide irrigation solution was the least toxic to fibroblasts, with survival rates equivalent to those of controls. The chlorous acid-chlorine dioxide disinfectant was 100% cytotoxic even when diluted 1:1 with phosphate buffered saline. S. aureus growth was inhibited by 1.0% and 0.5% chlorhexidine gluconate; concentrations of 0.05%, 0.01%, and 0.005% did not differ from sterile water controls. The chlorous acid-chlorine dioxide irrigation solution did not inhibit growth of S. aureus in brain-heart infusion broth. The chlorous acid-chlorine dioxide disinfectant inhibited growth of S. aureus.
Publication Date: 1991-09-01 PubMed ID: 1962413DOI: 10.1111/j.1532-950x.1991.tb01272.xGoogle Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This research study investigates the impact of varying concentrations of chlorhexidine gluconate, chlorous acid-chlorine dioxide irrigation solution, and chlorous acid-chlorine dioxide disinfectant on equine fibroblasts and Staphylococcus aureus, a common bacteria. The study discovered that different concentrations of these substances have varying effects on the viability of equine fibroblasts, and also their efficacy in inhibiting the growth of Staphylococcus aureus.
Examination Process
The study involved exposing equine fibroblasts and Staphylococcus aureus to different concentrations of three substances, namely, chlorhexidine gluconate and two types of chlorous acid-chlorine dioxide solutions. Exposure lasted for 30 minutes.
- Cell viability was assessed through trypsinization, staining with trypan blue, and counting cells which did not absorb the stain.
- Phosphate buffered saline controls were used for comparison purposes.
Impact on Equine Fibroblasts
The research provided insight into how the viability of equine fibroblasts was affected by each substance.
- High concentrations of 1.0% and 0.5% chlorhexidine gluconate were found to kill all fibroblast cells.
- Decreasing the concentration of chlorhexidine gluconate improved the survival rate linearly, with 50% cell survival observed at 0.005%.
- The chlorous acid-chlorine dioxide irrigation solution showed the least toxicity to fibroblasts, with cell survival rates similar to the controls.
- The chlorous acid-chlorine dioxide disinfectant, on the other hand, was completely cytotoxic, killing all cells in a 1:1 dilution with phosphate buffered saline.
Impact on Staphylococcus aureus
How the substances impacted Staphylococcus aureus growth was also a prominent component of the study.
- Staphylococcus aureus growth was hindered by 1.0% and 0.5% chlorhexidine gluconate.
- Lower concentrations of 0.05%, 0.01%, and 0.005% did not show much difference from the sterile water controls.
- The chlorous acid-chlorine dioxide irrigation solution did not impede the growth of Staphylococcus aureus in the brain-heart infusion broth.
- Conversely, the chlorous acid-chlorine dioxide disinfectant was effective in inhibiting the growth of Staphylococcus aureus.
Cite This Article
APA
Redding WR, Booth LC.
(1991).
Effects of chlorhexidine gluconate and chlorous acid-chlorine dioxide on equine fibroblasts and Staphylococcus aureus.
Vet Surg, 20(5), 306-310.
https://doi.org/10.1111/j.1532-950x.1991.tb01272.x Publication
Researcher Affiliations
- Department of Veterinary Clinical Sciences, Veterinary Teaching Hospital, Iowa State University, Ames.
MeSH Terms
- Animals
- Cell Line
- Cell Survival / drug effects
- Chlorhexidine / analogs & derivatives
- Chlorhexidine / pharmacology
- Chlorides / pharmacology
- Chlorine / pharmacology
- Chlorine Compounds
- Colony Count, Microbial
- Disinfectants / pharmacology
- Drug Combinations
- Fibroblasts / drug effects
- Horses
- Oxides / pharmacology
- Skin / cytology
- Staphylococcus aureus / drug effects
- Staphylococcus aureus / growth & development
Citations
This article has been cited 1 times.- Láng O, Nagy KS, Láng J, Perczel-Kovách K, Herczegh A, Lohinai Z, Varga G, Kőhidai L. Comparative study of hyperpure chlorine dioxide with two other irrigants regarding the viability of periodontal ligament stem cells. Clin Oral Investig 2021 May;25(5):2981-2992.
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
