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Acta veterinaria Scandinavica1981; 22(3-4); 472-479; doi: 10.1186/BF03548672

Effects of different anticoagulants on determination of erythrocyte glutathione peroxidase.

Abstract: Glutathione peroxidase (GSH-Px) was determined in whole blood from cows, goats and horses using cumenehydroperoxide as substrate. Heparin was found to be the most suitable anticoagulant. The highest activities of GSH-Px were found with high concentrations of heparin in the blood samples (1000 and 1250 IU/ml of blood). Sodium fluoride and especially EDTA and sodium citrate gave lower activities of the enzyme. Storage of the blood samples at room temperature (~20°C) or in a refrigerator (~5°C) for 3 days resulted in significantly lower activities of the enzyme, especially in horse blood. Glutationperoxidas (GSH-Px) bestämdes i helblod från kor, getter och hästar. Cuminhydroperoxid användes som substrat vid bestämningarna som utfördes i en LKB 8600 Rection Rate Analyzed. Heparin var det mest lämpliga antikoagulantium som testades. De högsta aktiviteterna av enzymet uppmättes då höga halter av heparin fanns i blodprovet (1000 och 1250 ΙΕ/ml blod). Natriumfluorid men specieilt EDTA och natriumcitrat gav lägre enzymaktiviteter vid samtidig bestämning på samma blodprov. Förvaring av blodp roverna i rumstemperatur (20°C) eller i kylskåp (5°C) under 3 dagar gav signifikant lägre enzymaktiviteter. Detta var specieilt tydligt i hästblod.
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Publication Date: 1981-01-01 PubMed ID: 6807069PubMed Central: PMC8300441DOI: 10.1186/BF03548672Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study investigates the effect of different anticoagulants on the determination of a specific enzyme, glutathione peroxidase, in the blood of cows, goats, and horses. The findings show that heparin was found to be the most effective anticoagulant, while others such as sodium fluoride, EDTA, and sodium citrate led to lower enzyme activities. Also, the storage temperature of blood samples affected the activities of this enzyme.

Using Anticoagulants for Enzyme Determination

  • The research focused on the measurement of an enzyme called Glutathione peroxidase (GSH-Px) found in the whole blood from cows, goats, and horses.
  • The study used a substrate called cumenehydroperoxide for the determination of the enzyme’s existence.
  • In order to prevent coagulation or clotting of the blood samples that may interfere with the enzyme detection, different anticoagulants were used, which included heparin, sodium fluoride, EDTA, and sodium citrate.

Efficacy of Heparin as an Anticoagulant

  • Results from the study indicated that heparin was the most effective anticoagulant for the purpose of enzyme determination.
  • The research showed that high concentrations of heparin in the blood samples resulted in the detection of highest activities of GSH-Px. This was specifically observed at concentrations of 1000 and 1250 IU/ml of blood.

Lower Efficacy of other Anticoagulants

  • The research found that the use of other anticoagulants like sodium fluoride, EDTA, and sodium citrate resulted in lower activities of the GSH-Px enzyme. This indicates their lesser efficacy as compared to heparin in the determination of this specific enzyme.

Impact of Storage Conditions on Enzyme Activities

  • The research also assessed the impact of storage conditions, such as temperature, on the activities of the GSH-Px enzyme.
  • It was observed that storage of the blood samples at room temperature (~20°C) or in the refrigerator (~5°C) for 3 days resulted in significantly lower enzyme activities.
  • This decline in enzyme activity with storage was especially noticeable in horse blood, highlighting that different species may respond differently to storage conditions.

Cite This Article

APA
Hussein KS, Jones BE. (1981). Effects of different anticoagulants on determination of erythrocyte glutathione peroxidase. Acta Vet Scand, 22(3-4), 472-479. https://doi.org/10.1186/BF03548672

Publication

Acta veterinaria Scandinavica
ISSN: 0044-605X
NlmUniqueID: 0370400
Country: England
Language: English
Volume: 22
Issue: 3-4
Pages: 472-479

Researcher Affiliations

Hussein, K S
    Jones, B E

      MeSH Terms

      • Animals
      • Anticoagulants / pharmacology
      • Blood Preservation / veterinary
      • Cattle / blood
      • Citrates / pharmacology
      • Citric Acid
      • Edetic Acid / pharmacology
      • Erythrocytes / drug effects
      • Erythrocytes / enzymology
      • Female
      • Glutathione Peroxidase / blood
      • Goats / blood
      • Heparin / pharmacology
      • Horses / blood
      • Peroxidases / blood
      • Sodium Fluoride / pharmacology

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      Citations

      This article has been cited 2 times.
      1. Hussein KS, Jones BE. Effects of selenium administration on erythrocyte and blood plasma glutathione peroxidase activity in goats. Acta Vet Scand 1982;23(4):559-64.
        doi: 10.1186/BF03546774pubmed: 7168432google scholar: lookup
      2. Agergaard N, Jensen PT. Procedure for blood glutathione peroxidase determination in cattle and swine. Acta Vet Scand 1982;23(4):515-27.
        doi: 10.1186/BF03546770pubmed: 7168428google scholar: lookup
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