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Home / Equine Research Bank / J Vet Sci / Effects of hydrocortisone and aminophylline on the aggregati...
Journal of veterinary science2011; 12(3); 215-219; doi: 10.4142/jvs.2011.12.3.215

Effects of hydrocortisone and aminophylline on the aggregation of equine platelets in vitro.

Abstract: The purpose of this study was to evaluate in vitro the effects of hydrocortisone and aminophylline on adenosine diphosphate (ADP)-induced platelet aggregation in horses. Blood samples from 30 healthy Thoroughbred horses were collected by via jugular venipuncture to assess platelet aggregation. Platelet-rich and platelet-poor plasma were prepared from all samples by centrifugation and divided into three different aliquots. In the first aliquot, platelet aggregation was measured after platelet activation with 1 µM and 0.5 µM ADP (Group A). In the other two aliquots, the effect of a 10 min preincubation with hydrocortisone (Group B) or aminophylline (Group C) on ADP-induced aggregation at final ADP concentrations of 1 µM and 0.5 µM was observed. Platelet aggregation, recorded by an aggregometer, was evaluated by measuring the maximum degree of platelet aggregation and the initial velocities of platelet aggregation were obtained. Our results demonstrated the inhibitory effect of hydrocortisone and the induction effect of aminophylline on equine platelet responses in vitro.
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Publication Date: 2011-09-08 PubMed ID: 21897093PubMed Central: PMC3165149DOI: 10.4142/jvs.2011.12.3.215Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The researchers conducted a study to understand the influence of hydrocortisone and aminophylline on the process of platelet aggregation in horse blood samples. Their findings suggest that while hydrocortisone inhibits this process, aminophylline actually induces it.

Objective of the Research

  • This research was aimed at evaluating the effects of hydrocortisone and aminophylline on the aggregation of platelets in horses in a controlled laboratory setting, specifically how these substances interact with adenosine diphosphate (ADP)-induced platelet aggregation.

Methodology of the Study

  • Blood samples from 30 healthy Thoroughbred horses were collected via jugular venipuncture and used for the assessment of platelet aggregation.
  • The samples were prepared as platelet-rich and platelet-poor plasma through a process called centrifugation.
  • The plasma samples were divided into three different aliquots for different test groups (A, B, C).
  • In Group A, platelet aggregation was measured after activation with ADP at concentrations of 1 µM and 0.5 µM.
  • For the samples in Group B and C, the effects of pre-incubation with hydrocortisone (Group B) and aminophylline (Group C) on ADP-induced aggregation was observed at final ADP concentrations of 1 µM and 0.5 µM.
  • An aggregometer, a device that measures the aggregation of particles, was used to record platelet aggregation. This was evaluated by observing the maximum degree of aggregation and the initial velocities of platelet aggregation.

Research Findings

  • The results obtained illustrated that hydrocortisone has an inhibitive effect on the aggregation of platelets in vitro – meaning in a lab-controlled environment.
  • Contrarily, aminophylline was found to have an induction effect on platelet responses, promoting or speeding up the process in vitro. They have drawn these conclusions based on differences between platelet aggregation in samples pre-incubated with the two substances compared to the base levels in Group A.

Cite This Article

APA
Casella S, Giudice E, Giannetto C, Marafioti S, Piccione G. (2011). Effects of hydrocortisone and aminophylline on the aggregation of equine platelets in vitro. J Vet Sci, 12(3), 215-219. https://doi.org/10.4142/jvs.2011.12.3.215

Publication

Journal of veterinary science
ISSN: 1976-555X
NlmUniqueID: 100964185
Country: Korea (South)
Language: English
Volume: 12
Issue: 3
Pages: 215-219

Researcher Affiliations

Casella, Stefania
  • Department of Experimental Science and Applied Biotechnology, Laboratory of Chronophysiology, University of Messina, Messina 98168, Italy.
Giudice, Elisabetta
    Giannetto, Claudia
      Marafioti, Simona
        Piccione, Giuseppe

          MeSH Terms

          • Adenosine Diphosphate / pharmacology
          • Aminophylline / pharmacology
          • Animals
          • Anti-Inflammatory Agents / pharmacology
          • Female
          • Horses / physiology
          • Hydrocortisone / pharmacology
          • Male
          • Platelet Aggregation / drug effects

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          Citations

          This article has been cited 6 times.
          1. Kim S, Chaudhary PK, Kim S. Role of Prednisolone in Platelet Activation by Inhibiting TxA(2) Generation through the Regulation of cPLA(2) Phosphorylation. Animals (Basel) 2023 Apr 11;13(8).
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          2. Torfs SC, Maes AA, Delesalle CJ, Pardon B, Croubels SM, Deprez P. Plasma serotonin in horses undergoing surgery for small intestinal colic. Can Vet J 2015 Feb;56(2):178-84.
            pubmed: 25694668
          3. Signorello MG, Leoncini G. The Cortisol Effect on the NO/cGMP Pathway. Int J Mol Sci 2025 Feb 8;26(4).
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          4. Kim S, Lee D, Chaudhary PK, Kim H, Kang BT, Kim S. Comprehensive characterization of platelet function in dogs with hyperadrenocorticism. Haematologica 2025 Aug 1;110(8):1834-1844.
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          5. El-Tareef FS, Abo-El-Sooud K, Karmi M, Hafez A. Effect of theophylline on serum and milk pharmacokinetics of tylosin following intramuscular administration in lactating goats. BMC Vet Res 2024 Jun 8;20(1):251.
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          6. Signorello MG, Ravera S, Leoncini G. Oxidative Stress Induced by Cortisol in Human Platelets. Int J Mol Sci 2024 Mar 28;25(7).
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