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Home / Equine Research Bank / Am J Vet Res / Effects of in vitro exposure to hay dust on expression of in...
American journal of veterinary research2007; 68(12); 1361-1369; doi: 10.2460/ajvr.68.12.1361

Effects of in vitro exposure to hay dust on expression of interleukin-17, -23, -8, and -1beta and chemokine (C-X-C motif) ligand 2 by pulmonary mononuclear cells isolated from horses chronically affected with recurrent airway disease.

Abstract: To examine effects of in vitro exposure to solutions of hay dust, lipopolysaccharide (LPS), or beta-glucan on cytokine expression in pulmonary mononuclear cells isolated from healthy horses and horses with recurrent airway obstruction (RAO). Methods: 8 RAO-affected and 7 control horses (experiment 1) and 6 of the RAO-affected and 5 of the control horses (experiment 2). Methods: Bronchoalveolar lavage cells were isolated from horses that had been stabled and fed dusty hay for 14 days. Pulmonary mononuclear cells were incubated for 24 (experiment 1) or 6 (experiment 2) hours with PBS solution or solutions of hay dust, beta-glucan, or LPS. Gene expression of interleukin (IL)-17, IL-23(p19 and p40 subunits), IL-8, IL-1beta, and chemokine (C-X-C motif) ligand 2 (CXCL2) was measured with a kinetic PCR assay. Results: Treatment with the highest concentration of hay dust solution for 6 or 24 hours increased expression of IL-23(p19 and p40), IL-8, and IL-1beta in cells from both groups of horses and increased early expression of IL-17 and CXCL2 in RAO-affected horses. Lipopolysaccharide upregulated early expression of IL-23(p40) and IL-8 in cells from both groups of horses but only late expression of these cytokines in cells from RAO-affected horses. Treatment with beta-glucan failed to increase cytokine expression at 6 or 24 hours. Conclusions: Cells from RAO-affected horses were not more responsive to the ligands tested than were cells from control horses, which suggests a minimal role of mononuclear cells in propagation of airway neutrophilia in horses with chronic RAO.
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Publication Date: 2007-12-07 PubMed ID: 18052742DOI: 10.2460/ajvr.68.12.1361Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't
  • Research Support
  • U.S. Gov't
  • Non-P.H.S.

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research is about the effects of in vitro exposure to hay dust on cytokine expression in the lung cells of healthy horses and those suffering from recurrent airway obstruction and argues that mononuclear cells may not play a significant in the progression of the disease.

Research Context

  • The study was conducted to examine the effects of exposure to hay dust, lipopolysaccharide (LPS), or beta-glucan on cytokine expression in lung cells (referred to as pulmonary mononuclear cells).
  • The tested cells were isolated from healthy horses and horses suffering from recurrent airway obstruction (RAO), a chronic respiratory condition common in horses.

Methodology

  • The researchers isolated bronchoalveolar lavage cells from horses that had been stabled and fed dusty hay for 14 days.
  • The isolated lung cells were then exposed in vitro (in a controlled lab setting) to different compounds such as hay dust, beta-glucan, or LPS for a period of 6 to 24 hours.
  • The gene expression of multiple cytokines – IL-17, IL-23(p19 and p40 subunits), IL-8, IL-1beta, and chemokine (C-X-C motif) ligand 2 (CXCL2) was measured using a kinetic PCR assay.

Results

  • Exposing the cells to the highest concentration of hay dust solution for 6 or 24 hours increased the expression of IL-23(p19 and p40), IL-8, and IL-1beta in cells from both groups of horses.
  • The hay dust also increased early expression of IL-17 and CXCL2 in horses with RAO.
  • LPS upregulated the early expression of IL-23(p40) and IL-8 in cells from both groups of horses but only increased the late expression of these cytokines in cells from horses with RAO.
  • Meanwhile, beta-glucan did not influence cytokine expression at either the 6 or 24 hour timepoints.

Conclusions

  • The researchers found that the cells from horses with RAO were not noticeably more responsive to the tested ligands than the cells from healthy horses.
  • The results suggest that mononuclear cells may not play a significant role in propagating airway neutrophilia (an increase in the number of a type of white blood cells in the airways) in horses with chronic RAO.

Cite This Article

APA
Ainsworth DM, Wagner B, Erb HN, Young JC, Retallick DE. (2007). Effects of in vitro exposure to hay dust on expression of interleukin-17, -23, -8, and -1beta and chemokine (C-X-C motif) ligand 2 by pulmonary mononuclear cells isolated from horses chronically affected with recurrent airway disease. Am J Vet Res, 68(12), 1361-1369. https://doi.org/10.2460/ajvr.68.12.1361

Publication

American journal of veterinary research
ISSN: 0002-9645
NlmUniqueID: 0375011
Country: United States
Language: English
Volume: 68
Issue: 12
Pages: 1361-1369

Researcher Affiliations

Ainsworth, Dorothy M
  • Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.
Wagner, Bettina
    Erb, Hollis N
      Young, Jean C
        Retallick, Danielle E

          MeSH Terms

          • Animals
          • Cells, Cultured
          • Chemokines, CX3C / genetics
          • Chemokines, CX3C / metabolism
          • Dust
          • Gene Expression Regulation
          • Horse Diseases / metabolism
          • Horses
          • Interleukin-17 / genetics
          • Interleukin-17 / metabolism
          • Interleukin-1beta / genetics
          • Interleukin-1beta / metabolism
          • Interleukin-23 / genetics
          • Interleukin-23 / metabolism
          • Interleukin-9 / genetics
          • Interleukin-9 / metabolism
          • Leukocytes, Mononuclear / metabolism
          • Lung / cytology
          • Respiratory Tract Diseases / veterinary

          Citations

          This article has been cited 11 times.
          1. Kang H, Lee GKC, Bienzle D, Arroyo LG, Sears W, Lillie BN, Beeler-Marfisi J. Equine alveolar macrophages and monocyte-derived macrophages respond differently to an inflammatory stimulus.. PLoS One 2023;18(3):e0282738.
            doi: 10.1371/journal.pone.0282738pubmed: 36920969google scholar: lookup
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            doi: 10.4049/jimmunol.2001354pubmed: 33952617google scholar: lookup
          4. Hue E, Orard M, Toquet MP, Depecker M, Couroucé A, Pronost S, Paillot R, Richard EA. Asymmetrical Pulmonary Cytokine Profiles Are Linked to Bronchoalveolar Lavage Fluid Cytology of Horses With Mild Airway Neutrophilia.. Front Vet Sci 2020;7:226.
            doi: 10.3389/fvets.2020.00226pubmed: 32391392google scholar: lookup
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            doi: 10.1186/s12917-018-1636-4pubmed: 30442129google scholar: lookup
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            doi: 10.7717/peerj.5759pubmed: 30324028google scholar: lookup
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            doi: 10.3390/ijms18122612pubmed: 29206130google scholar: lookup
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            doi: 10.1371/journal.pone.0154755pubmed: 27138006google scholar: lookup
          10. Sarmiento J, Perez B, Morales N, Henriquez C, Vidal L, Folch H, Galecio JS, Morán G. Apoptotic effects of tamoxifen on leukocytes from horse peripheral blood and bronchoalveolar lavage fluid.. Vet Res Commun 2013 Dec;37(4):333-8.
            doi: 10.1007/s11259-013-9571-0pubmed: 23846832google scholar: lookup
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            doi: 10.1186/1746-6148-7-48pubmed: 21843342google scholar: lookup
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