Effects of in vitro exposure to hay dust on expression of interleukin-23, -17, -8, and -1beta and chemokine (C-X-C motif) ligand 2 by pulmonary mononuclear cells from horses susceptible to recurrent airway obstruction.
Abstract: To examine gene expression of selected cytokines in pulmonary mononuclear cells isolated from healthy horses and horses susceptible to recurrent airway obstruction (RAO), and to determine whether interleukin (IL)-17 and IL-23 were associated with pulmonary inflammation. Methods: 6 RAO-susceptible and 5 healthy horses. Methods: Bronchoalveolar lavage cells were retrieved from horses that were stabled and fed dusty hay for 24 hours. Lavage cells devoid of neutrophils were incubated for 24 hours with solutions of PBS, hay dust, lipopolysaccharide, or B-glucan. Gene expression of IL-17, IL-23 (p19 and p40 subunits), IL-8, IL-1B, chemokine (C-X-C motif) ligand 2 (CXCL2), and B-actin was measured by use of real-time reverse transcription PCR assays. Results: The degree of inherent expression of target genes in bronchoalveolar lavage cells treated with PBSS was not different between the 2 groups of horses. Relative to exposure to PBSS, exposure to the hay dust solution increased gene expression of all cytokines more than 2-fold in cells from both groups of horses, but the magnitudes of these increases were not different between the groups. Exposure to lipopolysaccharide solution increased gene expression of IL-8, CXCL2, and IL-1B in cells from RAO-susceptible horses, but this increase was not significantly different from that in cells from control horses. Exposure to B-glucan solution failed to increase gene expression in cells from either horse group, compared with gene expression when cells were exposed to PBSS. Conclusions: The acute pulmonary neutrophilia characteristic of RAO was not associated with an increase in upregulation of gene expression of chemokines in pulmonary mononuclear cells from disease-susceptible horses.
Publication Date: 2009-10-03 PubMed ID: 19795943DOI: 10.2460/ajvr.70.10.1277Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
- Research Support
- U.S. Gov't
- Non-P.H.S.
- Bronchoalveolar Lavage
- Chemokines
- Cytokines
- Diagnosis
- Disease Diagnosis
- Equine Diseases
- Equine Health
- Gene Expression
- Hay
- Horses
- Immune Response
- In Vitro Research
- Inflammatory Response
- Interleukins
- Lipopolysaccharide
- Neutrophils
- Pulmonary Health
- Real-Time PCR
- Recurrent Airway Obstruction
- Veterinary Medicine
- Veterinary Research
Summary
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The research study aimed to understand the effects of hay dust on the gene expression of certain types of cytokines in horses prone to recurrent airway obstruction (RAO) and healthy horses. The study found that hay dust exposure amplified the gene expression of all cytokines in horses, but severity was not different between RAO-susceptible and healthy horses. Exposure to certain substances resulted in increased gene expression in RAO-prone horses compared to control, but these findings weren’t significantly different. Notably, the characteristic inflammation of RAO was not linked to increased gene expression of chemokine in pulmonary cells in RAO-susceptible horses.
Study Design
- The researchers examined gene expression for cytokines in pulmonary mononuclear cells isolated from both healthy horses and those susceptible to recurrent airway obstruction (RAO).
- The target group consisted of six RAO-susceptible and five healthy horses.
- Bronchoalveolar lavage cells from horses stabled and fed on dusty hay for 24 hours were collected.
- These cells, once separated from neutrophils, were exposed to solutions of PBS, hay dust, lipopolysaccharide, or B-glucan for a day.
- Gene expression for IL-17, IL-23 (p19 and p40 subunits), IL-8, IL-1B, chemokine (C-X-C motif) ligand 2 (CXCL2), and B-actin was measured using real-time reverse transcription PCR assays.
Findings
- Without treatment, the inherent expression of target genes in bronchoalveolar lavage cells didn’t differ between the two groups.
- Exposure to hay dust solution increased by more than twofold the gene expression of all cytokines in cells from both groups of horses. However, the intensities of these increases didn’t differ between the groups.
- Exposure to lipopolysaccharide solution caused an increase in gene expression of IL-8, CXCL2, and IL-1B in cells from RAO-susceptible horses. Nevertheless, this increase wasn’t significantly different from that in cells from control horses.
- Exposure to B-glucan solution didn’t have any effect on gene expression in cells from either group of horses, as compared to gene expression when the cells were exposed to PBS.
Conclusions
- The study’s results indicate no significant association between the acute pulmonary neutrophilia characteristic of RAO and an increase in gene expression of chemokines in pulmonary mononuclear cells from RAO-prone horses.
- The study provides useful insights for understanding the genetic aspect of RAO in horses and the role of environmental factors in its development.
Cite This Article
APA
Reyner CL, Wagner B, Young JC, Ainsworth DM.
(2009).
Effects of in vitro exposure to hay dust on expression of interleukin-23, -17, -8, and -1beta and chemokine (C-X-C motif) ligand 2 by pulmonary mononuclear cells from horses susceptible to recurrent airway obstruction.
Am J Vet Res, 70(10), 1277-1283.
https://doi.org/10.2460/ajvr.70.10.1277 Publication
Researcher Affiliations
- Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.
MeSH Terms
- Airway Obstruction / immunology
- Airway Obstruction / veterinary
- Animals
- Chemokines, CXC / genetics
- Chemokines, CXC / metabolism
- Dust / immunology
- Female
- Gene Expression Regulation / immunology
- Horse Diseases / immunology
- Horse Diseases / metabolism
- Horses
- Interleukin-17 / genetics
- Interleukin-17 / metabolism
- Interleukin-1beta / genetics
- Interleukin-1beta / metabolism
- Interleukin-23 / genetics
- Interleukin-23 / metabolism
- Interleukin-8 / genetics
- Interleukin-8 / metabolism
- Leukocytes, Mononuclear / metabolism
- Male
Citations
This article has been cited 4 times.- Gressler AE, Lübke S, Wagner B, Arnold C, Lohmann KL, Schnabel CL. Comprehensive Flow Cytometric Characterization of Bronchoalveolar Lavage Cells Indicates Comparable Phenotypes Between Asthmatic and Healthy Horses But Functional Lymphocyte Differences.. Front Immunol 2022;13:896255.
- Morini M, Peli A, Rinnovati R, Magazzù G, Romagnoli N, Spadari A, Pietra M. Immunohistochemical Expression of Neurokinin-A and Interleukin-8 in the Bronchial Epithelium of Horses with Severe Equine Asthma Syndrome during Asymptomatic, Exacerbation, and Remission Phase.. Animals (Basel) 2021 May 12;11(5).
- Larson EM, Babasyan S, Wagner B. IgE-Binding Monocytes Have an Enhanced Ability to Produce IL-8 (CXCL8) in Animals with Naturally Occurring Allergy.. J Immunol 2021 May 15;206(10):2312-2321.
- Karagianni AE, Kapetanovic R, McGorum BC, Hume DA, Pirie SR. The equine alveolar macrophage: functional and phenotypic comparisons with peritoneal macrophages.. Vet Immunol Immunopathol 2013 Oct 1;155(4):219-28.
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