Effects of in vivo administration of testosterone propionate on in vitro production of follicle-stimulating hormone and luteinizing hormone by pituitaries of pony mares.
Abstract: The in vitro incorporation of [3H]leucine into immunoprecipitable follicle-stimulating hormone (FSH) and luteinizing hormone (LH) was assessed for pituitaries from pony mares treated with testosterone propionate (TP) or oil (controls). Mares were treated every other day with TP (n = 4) at 350 micrograms/kg of body weight or with an equivalent volume of oil (n = 4). One day following the sixth injection of TP, each mare received an intravenous injection of gonadotropin releasing hormone (GnRH) at 1.0 micrograms/kg body weight and was bled frequently for 4 h. Treatment of mares with TP reduced FSH (P less than 0.05) and LH (P less than 0.01) concentrations in daily blood samples and increased (P less than 0.01) the amount of FSH secreted in response to GnRH compared with control mares. Incorporation of [3H]leucine into immunoprecipitable FSH was also greater (P less than 0.01) in pituitaries from TP-treated mares compared with control mares on both a per mg tissue and per anterior pituitary basis. The amount of LH secreted after GnRH, the amount left in the pituitary and the incorporation of [3H]leucine into LH were not affected by treatment. These results confirm earlier conclusions drawn from indirect evidence that androgens increase the production of FSH in the mare.
Publication Date: 1984-04-01 PubMed ID: 6426537DOI: 10.1095/biolreprod30.3.673Google Scholar: Lookup
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- Journal Article
Summary
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This study examines the effect of the hormone testosterone propionate on hormone production in the pituitary glands of mares. The results indicate that testosterone propionate increases the production of follicle-stimulating hormone while not significantly affecting the production of luteinizing hormone.
Method and Treatment
- The study used pony mares that were divided into two groups. One group was treated with testosterone propionate (TP), while the other group functioned as the control and was treated with oil.
- The mares in the TP group were treated every other day with an amount of TP that was proportional to their body weight.
- After six injections of TP, each mare was given an injection of gonadotropin-releasing hormone (GnRH) and subsequently monitored and bled for a 4-hour period.
Findings and Interpretation
- Testosterone propionate treatment caused a decrease in follicle-stimulating hormone (FSH) and luteinizing hormone (LH) concentrations in the daily blood samples taken from the mares. Despite this, the amount of FSH produced in response to the injection of GnRH was significantly higher in treated mares compared to control mares.
- The research utilized a process known as ‘3H-leucine incorporation’ to measure hormone production in the pituitary. The results showed that mares treated with TP had a significantly higher level of 3H-leucine incorporation into FSH than the control group, indicating that TP treatment increases the production of FSH.
- The study observed that TP treatment did not significantly impact the amount of LH released after the injection of GnRH, the amount remaining in the pituitary, or the level of 3H-leucine incorporation into LH.
- The findings of this study support previous indirect evidence suggesting that androgens, such as TP, stimulate the production of FSH in mares.
Conclusion
- This study provides direct scientific evidence that testosterone propionate, an androgen, increases the production of follicle-stimulating hormone in pony mares. The results are significant as they support previously inferred conclusions and provide a deeper understanding of hormone production regulation in equine species.
Cite This Article
APA
Reville-Moroz SI, Thompson DL, Archbald LF, Olsen LM.
(1984).
Effects of in vivo administration of testosterone propionate on in vitro production of follicle-stimulating hormone and luteinizing hormone by pituitaries of pony mares.
Biol Reprod, 30(3), 673-678.
https://doi.org/10.1095/biolreprod30.3.673 Publication
Researcher Affiliations
MeSH Terms
- Animals
- Castration
- Female
- Follicle Stimulating Hormone / metabolism
- Horses
- Luteinizing Hormone / metabolism
- Pituitary Gland, Anterior / drug effects
- Pituitary Gland, Anterior / metabolism
- Pituitary Hormone-Releasing Hormones / pharmacology
- Testosterone / pharmacology
Citations
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