Effects of ischemia and dimethyl sulfoxide on equine jejunal vascular resistance, oxygen consumption, intraluminal pressure, and potassium loss.
Abstract: Physiologic effects of 1 hour of ischemia and 1 hour of reperfusion on equine jejunum and protective effects of systemic administration of dimethyl sulfoxide (DMSO, 1 g/kg of body weight) were investigated in 18 ponies, using neurally intact segments of jejunum perfused at constant flow with heparinized blood. Ponies were allotted to 4 groups: group 1, saline solution administered (control, n = 3); group 2, DMSO administered (DMSO, n = 3); group 3, ischemia induced and saline solution administered (ischemia, n = 6); and group 4, ischemia induced and DMSO administered (ischemia-DMSO, n = 6). Intestinal vascular resistance (R, mm of Hg/ml/min/100 g), oxygen consumption (VO2, ml/min/100 g), frequency and amplitude of rhythmic changes in intraluminal pressure, intestinal compliance (C, ml/mm of Hg), and arteriovenous potassium concentration difference (delta AV [K+], mEq/L) were determined and compared with stable preischemic values within groups. There were no significant changes in any variable in ponies of groups 1 or 2. In ponies of group 3, significant (P less than or equal to 0.05) changes included: an initial increase in R during reperfusion, followed by a decrease to values below preischemic values by 15 minutes of reperfusion; decreased VO2 during the entire reperfusion period; increased amplitude of rhythmic contractions during initial reperfusion; decreased frequency of rhythmic contractions during ischemia; and increased delta AV [K+] during initial reperfusion. Changes in ponies of group 4 were identical to changes in ponies of group 3, with the exception that DMSO administration prevented the decrease in R during reperfusion.(ABSTRACT TRUNCATED AT 250 WORDS)
Publication Date: 1989-03-01 PubMed ID: 2930025
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- Journal Article
Summary
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The research investigates the physiological effects of ischemia on horse intestine – particularly the equine jejunum. It also evaluated the protective impacts of the compound dimethyl sulfoxide (DMSO) on the jejunum during ischemia and reperfusion, with the study conducted on a sample of 18 ponies.
Research Design and Groups
- The study used 18 ponies and divided them into four groups. The divisions allowed for controlled and variable experiments, where ischemia was induced in half of the groups and DMSO administered to the other half.
- Group 1 was the control group administered saline solution; Group 2 received only DMSO; Group 3 had ischemia induced, followed by saline administration; Group 4 had ischemia induced, followed by DMSO administration.
- The equine jejunum was studied using neurally intact segments perfused at constant flow with heparinized blood. This meant that the blood was treated to prevent clotting, ensuring the smooth flow in the experiment device.
Parameters and Variables
- The parameters observed during the experiment included intestinal vascular resistance, oxygen consumption, the frequency and amplitude of rhythmic changes in intraluminal pressure, intestinal compliance, and arteriovenous potassium concentration difference.
- These variables were measured and compared before and after the induction of ischemia and during the reperfusion phase, for both ischemic and non-ischemic sections.
Results
- Results showed that there were no significant changes in groups 1 and 2, meaning that saline and DMSO administration without ischemia did not affect the observed parameters.
- In group 3, ischemia significantly affected all the variables, resulting in an increase in vascular resistance during reperfusion, decrease in oxygen consumption during reperfusion, and changes in rhythmic contractions along with an increase in potassium concentration during reperfusion.
- In group 4, ischemia-induced changes were similar to group 3, but DMSO administration prevented the decrease in vascular resistance during reperfusion, indicating a protective effect on the equine jejunum during the recovery from ischemia.
This study provided insights into the physiology of equine intestine during conditions of limited blood supply and the potential therapeutic benefits of DMSO in managing such conditions.
Cite This Article
APA
Arden WA, Stick JA, Parks AH, Chou CC, Slocombe RF.
(1989).
Effects of ischemia and dimethyl sulfoxide on equine jejunal vascular resistance, oxygen consumption, intraluminal pressure, and potassium loss.
Am J Vet Res, 50(3), 380-387.
Publication
Researcher Affiliations
- Department of Large Animal Clinical Sciences, Michigan State University, East Lansing 48824.
MeSH Terms
- Animals
- Dimethyl Sulfoxide / therapeutic use
- Gastrointestinal Motility
- Horse Diseases / drug therapy
- Horse Diseases / physiopathology
- Horses
- Ischemia / drug therapy
- Ischemia / physiopathology
- Ischemia / veterinary
- Jejunum / blood supply
- Jejunum / physiopathology
- Oxygen Consumption
- Potassium / metabolism
- Pressure
- Vascular Resistance
Citations
This article has been cited 1 times.- Moore RM, Muir WW, Rush BR. Systemic and colonic venous plasma biochemical alterations in horses during low-flow ischemia and reperfusion of the large colon. Can J Vet Res 1998 Jan;62(1):14-20.
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