Effects of media and promoters on different lipid peroxidation assays in stallion sperm.
Abstract: Effects of different media and promoters on lipid peroxidation (LPO) in viable stallion sperm have not been reported. Aims of this study were to determine effects of three media (INRA-96™, Equipro CoolGuard™, and Biggers, Whitten and Whittingham [BWW]), and promoters (iron sulfate-Fe; ultraviolet light-UV; or control-no exposure to promoters) on viable sperm LPO using four different flow cytometric assays (i.e., BODIPY, Liperfluo, 4-hydroxylnonenal [4HNE], malonaldehyde [MDA]). Significant media x promoter interactions were detected using the Liperfluo, 4HNE, and MDA assays (P < 0.05); therefore, data were sorted by media and by promoters. With inclusion of milk-based media, there were similar concentrations of LPO in control samples with use of all LPO assays. The effect of iron, as a promoter of LPO production, was media dependent, and milk-based media protected sperm from iron-induced LPO production when there were assessments with all assays. In contrast, iron promoted LPO in sperm diluted in BWW when there was use of in all assays, except BODIPY, probably because of the different target molecule with use of this assay. Ultraviolet light was the most potent LPO promoter with all media and assays evaluated. Data indicate milk-based extenders are generally more LPO-protective than BWW early in the LPO production pathway (based on BODIPY and Liperfluo assays), but are less protective during the later stages of LPO production (based on 4HNE and MDA assays). The use of different media and promoters of LPO allowed for determination of early and late stages of LPO in viable stallion sperm.
Copyright © 2019 Elsevier B.V. All rights reserved.
Publication Date: 2019-11-01 PubMed ID: 31785628DOI: 10.1016/j.anireprosci.2019.106199Google Scholar: Lookup
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- Journal Article
Summary
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The research article studies the effects of varying media and promoters on lipid peroxidation (LPO) – a process that can negatively affect the functioning of stallion sperm. The study conducts four different tests to determine whether different types of media and exposure to promoters such as iron sulfate and ultraviolet light can either enhance or inhibit LPO in viable stallion sperm.
Objectives and Methodology
- The primary objectives of the study were to investigate if three different media (INRA-96™, Equipro CoolGuard™, and Biggers, Whitten and Whittingham [BWW]) and promoters (iron sulfate, ultraviolet light, or no exposure as a control) affected lipid peroxidation in stallion sperm.
- The effects were evaluated using four different assays designed to measure LPO: BODIPY, Liperfluo, 4HNE, and MDA.
Results and Findings
- The data showed significant interactions between the media and promoter types in the Liperfluo, 4HNE, and MDA assays.
- When milk-based media were used, LPO was generally on par in the control samples across all testing methods.
- The effect of the iron promoter on increasing LPO production was contingent on the media type; milk-based media seemed to protect the sperm from iron-induced LPO across all four testing methods.
- Conversely, the iron promoter increased LPO in sperm diluted in BWW, except when the BODIPY assay was used.
- Ultraviolet light was found to be the most potent promoter of LPO, regardless of the assay method or media used.
Conclusion
- In conclusion, the study found that the media and promoters used affected both the initial and final stages of lipid peroxidation in stallion sperm.
- Milk-based media generally offered more protection against LPO than BWW, particularly in the early stages of the process, but this protection seemed to lessen in the later stages.
- These findings can guide decisions on selecting appropriate media and understanding how different promoters can influence lipid peroxidation in stallion sperm.
Cite This Article
APA
Ghosh S, Serafini R, Love CC, Teague SR, Hernández-Avilés C, LaCaze KA, Varner DD.
(2019).
Effects of media and promoters on different lipid peroxidation assays in stallion sperm.
Anim Reprod Sci, 211, 106199.
https://doi.org/10.1016/j.anireprosci.2019.106199 Publication
Researcher Affiliations
- Department of Large Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, TX, USA.
- Department of Large Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, TX, USA. Electronic address: rosanna.serafini@gmail.com.
- Department of Large Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, TX, USA.
- Department of Large Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, TX, USA.
- Department of Large Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, TX, USA.
- Department of Large Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, TX, USA.
- Department of Large Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, TX, USA.
MeSH Terms
- Animals
- Cryopreservation / veterinary
- Cryoprotective Agents / pharmacology
- Culture Media
- Horses / physiology
- Lipid Peroxidation / drug effects
- Male
- Reactive Oxygen Species
- Semen Preservation
- Spermatozoa / drug effects
- Spermatozoa / physiology
- Spermatozoa / radiation effects
- Ultraviolet Rays
Citations
This article has been cited 6 times.- Jing J, Peng Y, Fan W, Han S, Peng Q, Xue C, Qin X, Liu Y, Ding Z. Obesity-induced oxidative stress and mitochondrial dysfunction negatively affect sperm quality. FEBS Open Bio 2023 Apr;13(4):763-778.
- Najafi A, Mehdipour M, Mohammadi H, Mehdipour Z, Khorrami B, Nazari M. Effect of tempol and straw size on rooster sperm quality and fertility after post-thawing. Sci Rep 2022 Jul 16;12(1):12192.
- Zhang Q, Qu H, Chen Y, Luo X, Chen C, Xiao B, Ding X, Zhao P, Lu Y, Chen AF, Yu Y. Atorvastatin Induces Mitochondria-Dependent Ferroptosis via the Modulation of Nrf2-xCT/GPx4 Axis. Front Cell Dev Biol 2022;10:806081.
- Li Y, Zhang R, Wan Q, Hu R, Ma Y, Wang Z, Hou J, Zhang W, Tang BZ. Trojan Horse-Like Nano-AIE Aggregates Based on Homologous Targeting Strategy and Their Photodynamic Therapy in Anticancer Application. Adv Sci (Weinh) 2021 Dec;8(23):e2102561.
- Pintus E, Ros-Santaella JL. Impact of Oxidative Stress on Male Reproduction in Domestic and Wild Animals. Antioxidants (Basel) 2021 Jul 20;10(7).
- Mei H, Zhao L, Li W, Zheng Z, Tang D, Lu X, He Y. Inhibition of ferroptosis protects House Ear Institute-Organ of Corti 1 cells and cochlear hair cells from cisplatin-induced ototoxicity. J Cell Mol Med 2020 Oct;24(20):12065-12081.
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