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Home / Equine Research Bank / J Vet Intern Med / Effects of nebulized dexamethasone on the respiratory microb...
Journal of veterinary internal medicine2019; 34(1); 307-321; doi: 10.1111/jvim.15671

Effects of nebulized dexamethasone on the respiratory microbiota and mycobiota and relative equine herpesvirus-1, 2, 4, 5 in an equine model of asthma.

Abstract: Prolonged exposure to environmental antigens or allergens elicits an immune response in both healthy horses and those with mild asthma. Corticosteroids often are used to treat lower airway inflammation. Objective: To investigate the changes in equine herpesvirus (EHV)-1,2,4,5 glycoprotein B gene expression and changes in respiratory bacterial and fungal communities after nebulized dexamethasone treatment of horses with asthma. Methods: Horses with naturally occurring mild asthma (n = 16) and healthy control horses (n = 4). Methods: Prospective, randomized, controlled, blinded clinical trial. Polymerase chain reaction amplification of EHV-1,2,4,5 in bronchoalveolar lavage fluid, and 16S (microbiome) and ITS2 (mycobiome) genes with subsequent sequencing was performed on DNA extracted from nasal swabs and transendoscopic tracheal aspirates before and after 13 days treatment with nebulized dexamethasone (15 mg q24h) and saline (control). Results: Nebulized dexamethasone treatment decreased microbial diversity; relative abundance of 8 genera in the upper respiratory tract were altered. For both the microbiota and the mycobiota, environment had a dominant effect over treatment. Alternaria, an opportunistic pathogen and allergen in humans recognized as a risk factor for asthma, asthma severity, and exacerbations, was increased with treatment. Treatment affected relative quantification of the equine gamma herpesviruses (EHV-2 and -5); EHV-2 DNA levels increased and those of EHV-5 decreased. Conclusions: Nebulized dexamethasone treatment affected the upper respiratory tract microbiota, but not the mycobiota, which was overwhelmed by the effect of a sustained dusty environment.
© 2019 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals, Inc. on behalf of the American College of Veterinary Internal Medicine.
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Publication Date: 2019-12-03 PubMed ID: 31793692PubMed Central: PMC6979091DOI: 10.1111/jvim.15671Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article studies the impact of nebulized dexamethasone treatment on horses with asthma. It analyzes the effects on equine herpesvirus gene expression and the upper respiratory tract’s bacterial and fungal communities. Nebulized dexamethasone altered microbial diversity, affected relative quantities of equine gamma herpesviruses, and increased Alternaria, a human-recognized asthma risk factor.

About the Study

  • The study investigates the changes in horses’ respiratory microbiota and mycobiota, and equine herpesvirus (EHV)-1,2,4,5 glycoprotein B gene expression after treatment with nebulized dexamethasone.
  • The subjects of the research were horses with naturally occurring mild asthma (16 horses) and healthy control horses (4 horses).
  • The study was designed as a prospective, randomized, controlled, blinded clinical trial.

Methodology

  • The researchers performed PCR amplification of EHV-1,2,4,5 in bronchoalveolar lavage fluid, and 16S (microbiome) and ITS2 (mycobiome) genes.
  • The research was conducted on DNA extracted from nasal swabs and transendoscopic tracheal aspirates before and after a 13-day course of nebulized dexamethasone treatment and saline (control).

Results

  • The treatment with nebulized dexamethasone decreased microbial diversity and altered the relative abundance of 8 genera in the upper respiratory tract.
  • The study saw a rise in the level of Alternaria after dexamethasone treatment – an opportunistic pathogen and allergen recognized as a risk factor for asthma, the severity of asthma, and exacerbations in humans.
  • It was also found that dexamethasone treatment influenced the relative quantification of equine gamma herpesviruses (EHV-2 and -5), with an increase in EHV-2 DNA levels and a decrease in EHV-5 levels.

Conclusions

  • The study concluded that nebulized dexamethasone treatment had an impact on the upper respiratory tract’s microbiota. However, it did not significantly affect the mycobiota, as this was overwhelmed by the effect of a sustained dusty environment.

Cite This Article

APA
Bond SL, Workentine M, Hundt J, Gilkerson JR, Léguillette R. (2019). Effects of nebulized dexamethasone on the respiratory microbiota and mycobiota and relative equine herpesvirus-1, 2, 4, 5 in an equine model of asthma. J Vet Intern Med, 34(1), 307-321. https://doi.org/10.1111/jvim.15671

Publication

Journal of veterinary internal medicine
ISSN: 1939-1676
NlmUniqueID: 8708660
Country: United States
Language: English
Volume: 34
Issue: 1
Pages: 307-321

Researcher Affiliations

Bond, Stephanie L
  • Faculty of Veterinary Medicine, University of Calgary, Calgary, Alberta, Canada.
Workentine, Matthew
  • Faculty of Veterinary Medicine, University of Calgary, Calgary, Alberta, Canada.
Hundt, Jana
  • Faculty of Veterinary Medicine, University of Calgary, Calgary, Alberta, Canada.
Gilkerson, James R
  • Centre for Equine Infectious Disease, Melbourne Veterinary School, The University of Melbourne, Melbourne, Victoria, Australia.
Léguillette, Renaud
  • Faculty of Veterinary Medicine, University of Calgary, Calgary, Alberta, Canada.

MeSH Terms

  • Animals
  • Anti-Inflammatory Agents / therapeutic use
  • Asthma / drug therapy
  • Asthma / veterinary
  • Bacteria / drug effects
  • Dexamethasone / therapeutic use
  • Fungi / drug effects
  • Herpesviridae / classification
  • Herpesviridae / drug effects
  • Horse Diseases
  • Horses
  • Nebulizers and Vaporizers
  • Respiratory System / microbiology
  • Respiratory Therapy

Conflict of Interest Statement

Authors declare no conflict of interest.

References

This article includes 50 references
  1. Couëtil LL, Cardwell JM, Gerber V, Lavoie JP, Léguillette R, Richard EA. Inflammatory Airway Disease of Horses--Revised Consensus Statement.. J Vet Intern Med 2016 Mar-Apr;30(2):503-15.
    pmc: PMC4913592pubmed: 26806374doi: 10.1111/jvim.13824google scholar: lookup
  2. Bond S, Léguillette R, Richard EA, Couetil L, Lavoie JP, Martin JG, Pirie RS. Equine asthma: Integrative biologic relevance of a recently proposed nomenclature.. J Vet Intern Med 2018 Nov;32(6):2088-2098.
    pmc: PMC6271326pubmed: 30294851doi: 10.1111/jvim.15302google scholar: lookup
  3. Tremblay GM, Ferland C, Lapointe JM, Vrins A, Lavoie JP, Cormier Y. Effect of stabling on bronchoalveolar cells obtained from normal and COPD horses.. Equine Vet J 1993 May;25(3):194-7.
    pubmed: 8508745doi: 10.1111/j.2042-3306.1993.tb02941.xgoogle scholar: lookup
  4. Gerber V, Lindberg A, Berney C, Robinson NE. Airway mucus in recurrent airway obstruction--short-term response to environmental challenge.. J Vet Intern Med 2004 Jan-Feb;18(1):92-7.
    pubmed: 14765737doi: 10.1892/0891-6640(2004)18<92:amirao>2.0.co;2google scholar: lookup
  5. Leclere M, Lavoie-Lamoureux A, Gélinas-Lymburner E, David F, Martin JG, Lavoie JP. Effect of antigenic exposure on airway smooth muscle remodeling in an equine model of chronic asthma.. Am J Respir Cell Mol Biol 2011 Jul;45(1):181-7.
    pubmed: 20935189doi: 10.1165/rcmb.2010-0300ocgoogle scholar: lookup
  6. Pirie RS, Dixon PM, Collie DD, McGorum BC. Pulmonary and systemic effects of inhaled endotoxin in control and heaves horses.. Equine Vet J 2001 May;33(3):311-8.
    pubmed: 11352355doi: 10.2746/042516401776249732google scholar: lookup
  7. Desjardins I, Theoret C, Joubert P, Wagner B, Lavoie JP. Comparison of TGF-beta 1 concentrations in bronchoalveolar fluid of horses affected with heaves and of normal controls.. Vet Immunol Immunopathol 2004 Oct;101(3-4):133-41.
    pubmed: 15350743doi: 10.1016/j.vetimm.2004.03.008google scholar: lookup
  8. Bullone M, Lavoie JP. Asthma "of horses and men"--how can equine heaves help us better understand human asthma immunopathology and its functional consequences?. Mol Immunol 2015 Jul;66(1):97-105.
    pubmed: 25547716doi: 10.1016/j.molimm.2014.12.005google scholar: lookup
  9. Ivester KM, Couëtil LL, Zimmerman NJ. Investigating the link between particulate exposure and airway inflammation in the horse.. J Vet Intern Med 2014 Nov-Dec;28(6):1653-65.
    pmc: PMC4895611pubmed: 25273818doi: 10.1111/jvim.12458google scholar: lookup
  10. Gerber V, Robinson NE, Luethi S, Marti E, Wampfler B, Straub R. Airway inflammation and mucus in two age groups of asymptomatic well-performing sport horses.. Equine Vet J 2003 Jul;35(5):491-5.
    pubmed: 12875328doi: 10.2746/042516403775600424google scholar: lookup
  11. Ivester KM, Couëtil LL, Moore GE, Zimmerman NJ, Raskin RE. Environmental exposures and airway inflammation in young thoroughbred horses.. J Vet Intern Med 2014 May-Jun;28(3):918-24.
    pmc: PMC4895475pubmed: 24773603doi: 10.1111/jvim.12333google scholar: lookup
  12. Holcombe SJ, Jackson C, Gerber V, Jefcoat A, Berney C, Eberhardt S, Robinson NE. Stabling is associated with airway inflammation in young Arabian horses.. Equine Vet J 2001 May;33(3):244-9.
    pubmed: 11352345doi: 10.2746/042516401776249606google scholar: lookup
  13. Millerick-May ML, Karmaus W, Derksen FJ, Berthold B, Holcombe SJ, Robinson NE. Local airborne particulate concentration is associated with visible tracheal mucus in Thoroughbred racehorses.. Equine Vet J 2013 Jan;45(1):85-90.
    pubmed: 22494157doi: 10.1111/j.2042-3306.2012.00568.xgoogle scholar: lookup
  14. Elfman L, Riihimäki M, Pringle J, Wålinder R. Influence of horse stable environment on human airways.. J Occup Med Toxicol 2009 May 25;4:10.
    pmc: PMC2693518pubmed: 19467158doi: 10.1186/1745-6673-4-10google scholar: lookup
  15. Mair T, Derksen F. Chronic obstructive pulmonary disease: a review.. Equine Vet Educ 2000;12:35‐44.
  16. Wood JL, Newton JR, Chanter N, Mumford JA. Inflammatory airway disease, nasal discharge and respiratory infections in young British racehorses.. Equine Vet J 2005 May;37(3):236-42.
    pubmed: 15892233doi: 10.2746/0425164054530579google scholar: lookup
  17. Fortier G, van Erck E, Fortier C, Richard E, Pottier D, Pronost S, Miszczak F, Thiry E, Lekeux P. Herpesviruses in respiratory liquids of horses: putative implication in airway inflammation and association with cytological features.. Vet Microbiol 2009 Oct 20;139(1-2):34-41.
    pubmed: 19427139doi: 10.1016/j.vetmic.2009.04.021google scholar: lookup
  18. Newton JR, Wood JL, Chanter N. A case control study of factors and infections associated with clinically apparent respiratory disease in UK Thoroughbred racehorses.. Prev Vet Med 2003 Jul 30;60(1):107-32.
    pubmed: 12900152doi: 10.1016/s0167-5877(03)00085-0google scholar: lookup
  19. Busse WW, Lemanske RF Jr, Gern JE. Role of viral respiratory infections in asthma and asthma exacerbations.. Lancet 2010 Sep 4;376(9743):826-34.
    pmc: PMC2972660pubmed: 20816549doi: 10.1016/s0140-6736(10)61380-3google scholar: lookup
  20. Diaz-Mendez A, Viel L, Hewson J, Doig P, Carman S, Chambers T, Tiwari A, Dewey C. Surveillance of equine respiratory viruses in Ontario.. Can J Vet Res 2010 Oct;74(4):271-8.
    pmc: PMC2949340pubmed: 21197227
  21. Hue ES, Fortier GD, Fortier CI, Leon AM, Richard EA, Legrand LJ, Pronost SL. Detection and quantitation of equid gammaherpesviruses (EHV-2, EHV-5) in nasal swabs using an accredited standardised quantitative PCR method.. J Virol Methods 2014 Mar;198:18-25.
    pubmed: 24370678doi: 10.1016/j.jviromet.2013.12.008google scholar: lookup
  22. Hartley CA, Dynon KJ, Mekuria ZH, El-Hage CM, Holloway SA, Gilkerson JR. Equine gammaherpesviruses: perfect parasites?. Vet Microbiol 2013 Nov 29;167(1-2):86-92.
    pubmed: 23845734doi: 10.1016/j.vetmic.2013.05.031google scholar: lookup
  23. Fortier G, Richard E, Hue E, Fortier C, Pronost S, Pottier D, Lemaitre L, Lekeux P, Borchers K, Thiry E. Long-lasting airway inflammation associated with equid herpesvirus-2 in experimentally challenged horses.. Vet J 2013 Aug;197(2):492-5.
    pubmed: 23433569doi: 10.1016/j.tvjl.2012.12.027google scholar: lookup
  24. Haspel AD, Giguère S, Hart KA, Berghaus LJ, Davis JL. Bioavailability and tolerability of nebulised dexamethasone sodium phosphate in adult horses.. Equine Vet J 2018 Jan;50(1):85-90.
    pubmed: 28719014doi: 10.1111/evj.12724google scholar: lookup
  25. Mathieson D. How to Perform the Perfect Tracheal Wash.. Birmingham, United Kingdom: British Equine Veterinary Association Congress; 2012.
  26. Bond SL, Timsit E, Workentine M, Alexander T, Léguillette R. Upper and lower respiratory tract microbiota in horses: bacterial communities associated with health and mild asthma (inflammatory airway disease) and effects of dexamethasone.. BMC Microbiol 2017 Aug 23;17(1):184.
    pmc: PMC5569571pubmed: 28835202doi: 10.1186/s12866-017-1092-5google scholar: lookup
  27. Fernandez NJ, Hecker KG, Gilroy CV, Warren AL, Léguillette R. Reliability of 400-cell and 5-field leukocyte differential counts for equine bronchoalveolar lavage fluid.. Vet Clin Pathol 2013 Mar;42(1):92-8.
    pubmed: 23289790doi: 10.1111/vcp.12013google scholar: lookup
  28. Dynon KJ. Pathogenesis of Equine Herpesvirus 2 (EHV2) Infection and Cellular Processing of EHV2 Glycoprotein B.. Melbourne, Australia: University of Melbourne, Equine Infectious Diseases Laboratory, Equine Virology Group, School of Veterinary Science; 2010.
  29. Pfaffl MW, Horgan GW, Dempfle L. Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR.. Nucleic Acids Res 2002 May 1;30(9):e36.
    pmc: PMC113859pubmed: 11972351doi: 10.1093/nar/30.9.e36google scholar: lookup
  30. Léguillette R, Laviolette M, Bergeron C, Zitouni N, Kogut P, Solway J, Kachmar L, Hamid Q, Lauzon AM. Myosin, transgelin, and myosin light chain kinase: expression and function in asthma.. Am J Respir Crit Care Med 2009 Feb 1;179(3):194-204.
    pmc: PMC2633053pubmed: 19011151doi: 10.1164/rccm.200609-1367ocgoogle scholar: lookup
  31. Ewels P, Magnusson M, Lundin S, Käller M. MultiQC: summarize analysis results for multiple tools and samples in a single report.. Bioinformatics 2016 Oct 1;32(19):3047-8.
    pmc: PMC5039924pubmed: 27312411doi: 10.1093/bioinformatics/btw354google scholar: lookup
  32. Martin M. Cutadapt removes adapter sequences from high‐throughput sequencing reads.. EMBnet J 2011;17:10‐12.
  33. Callahan BJ, McMurdie PJ, Rosen MJ, Han AW, Johnson AJ, Holmes SP. DADA2: High-resolution sample inference from Illumina amplicon data.. Nat Methods 2016 Jul;13(7):581-3.
    pmc: PMC4927377pubmed: 27214047doi: 10.1038/nmeth.3869google scholar: lookup
  34. Team RC. R: A Language and Environment for Statistical Computing.. Vienna, Austria: R Foundation for Statistical Computing; 2016.
  35. Wang Q, Garrity GM, Tiedje JM, Cole JR. Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy.. Appl Environ Microbiol 2007 Aug;73(16):5261-7.
    pmc: PMC1950982pubmed: 17586664doi: 10.1128/aem.00062-07google scholar: lookup
  36. Nawrocki E. Structural RNA homology search and alignment using covariance models.. PhD thesis. Washington University in St. Louis 2009.
  37. Price MN, Dehal PS, Arkin AP. FastTree 2--approximately maximum-likelihood trees for large alignments.. PLoS One 2010 Mar 10;5(3):e9490.
    pmc: PMC2835736pubmed: 20224823doi: 10.1371/journal.pone.0009490google scholar: lookup
  38. McMurdie PJ, Holmes S. phyloseq: an R package for reproducible interactive analysis and graphics of microbiome census data.. PLoS One 2013;8(4):e61217.
    pmc: PMC3632530pubmed: 23630581doi: 10.1371/journal.pone.0061217google scholar: lookup
  39. Oksanen J, Blanchet FG, Kindt R. vegan: Community ecology package.. R package version 2011:117–118.
  40. Love MI, Huber W, Anders S. Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2.. Genome Biol 2014;15(12):550.
    pmc: PMC4302049pubmed: 25516281doi: 10.1186/s13059-014-0550-8google scholar: lookup
  41. Dauvillier J, Ter Woort F, van Erck-Westergren E. Fungi in respiratory samples of horses with inflammatory airway disease.. J Vet Intern Med 2019 Mar;33(2):968-975.
    pmc: PMC6430897pubmed: 30576012doi: 10.1111/jvim.15397google scholar: lookup
  42. Bush RK, Prochnau JJ. Alternaria-induced asthma.. J Allergy Clin Immunol 2004 Feb;113(2):227-34.
    pubmed: 14767434doi: 10.1016/j.jaci.2003.11.023google scholar: lookup
  43. Bacci E, Cianchetti S, Paggiaro PL, Carnevali S, Bancalari L, Dente FL, Di Franco A, Giannini D, Vagaggini B, Giuntini C. Comparison between hypertonic and isotonic saline-induced sputum in the evaluation of airway inflammation in subjects with moderate asthma.. Clin Exp Allergy 1996 Dec;26(12):1395-400.
    pubmed: 9027440
  44. Khan SY, O'Driscoll BR. Is nebulized saline a placebo in COPD?. BMC Pulm Med 2004 Sep 30;4:9.
    pmc: PMC526282pubmed: 15458566doi: 10.1186/1471-2466-4-9google scholar: lookup
  45. Fillion-Bertrand G, Dickson RP, Boivin R, Lavoie JP, Huffnagle GB, Leclere M. Lung Microbiome Is Influenced by the Environment and Asthmatic Status in an Equine Model of Asthma.. Am J Respir Cell Mol Biol 2019 Feb;60(2):189-197.
    pubmed: 30183323doi: 10.1165/rcmb.2017-0228ocgoogle scholar: lookup
  46. Lavoie JP, Léguillette R, Pasloske K, Charette L, Sawyer N, Guay D, Murphy T, Hickey GJ. Comparison of effects of dexamethasone and the leukotriene D4 receptor antagonist L-708,738 on lung function and airway cytologic findings in horses with recurrent airway obstruction.. Am J Vet Res 2002 Apr;63(4):579-85.
    pubmed: 11939323doi: 10.2460/ajvr.2002.63.579google scholar: lookup
  47. Leclere M, Lavoie-Lamoureux A, Joubert P, Relave F, Setlakwe EL, Beauchamp G, Couture C, Martin JG, Lavoie JP. Corticosteroids and antigen avoidance decrease airway smooth muscle mass in an equine asthma model.. Am J Respir Cell Mol Biol 2012 Nov;47(5):589-96.
    pubmed: 22721832doi: 10.1165/rcmb.2011-0363ocgoogle scholar: lookup
  48. Lavoie JP, Pasloske K, Joubert P, Cordeau ME, Mancini J, Girard Y, Friesen RW, Frenette R, Blouin M, Young RN, Hickey G. Lack of clinical efficacy of a phosphodiesterase-4 inhibitor for treatment of heaves in horses.. J Vet Intern Med 2006 Jan-Feb;20(1):175-81.
    pubmed: 16496938doi: 10.1892/0891-6640(2006)20[175:loceoa]2.0.co;2google scholar: lookup
  49. Bullone M, Vargas A, Elce Y, Martin JG, Lavoie JP. Fluticasone/salmeterol reduces remodelling and neutrophilic inflammation in severe equine asthma.. Sci Rep 2017 Aug 18;7(1):8843.
    pmc: PMC5562887pubmed: 28821845doi: 10.1038/s41598-017-09414-8google scholar: lookup
  50. Léguillette R, Tohver T, Bond SL, Nicol JA, McDonald KJ. Effect of Dexamethasone and Fluticasone on Airway Hyperresponsiveness in Horses With Inflammatory Airway Disease.. J Vet Intern Med 2017 Jul;31(4):1193-1201.
    pmc: PMC5508307pubmed: 28568169doi: 10.1111/jvim.14740google scholar: lookup

Citations

This article has been cited 12 times.
  1. Bond S, McMullen C, Timsit E, Léguillette R. Topography of the respiratory, oral, and guttural pouch bacterial and fungal microbiotas in horses. J Vet Intern Med 2023 Jan;37(1):349-360.
    doi: 10.1111/jvim.16612pubmed: 36607177google scholar: lookup
  2. Simões J, Batista M, Tilley P. The Immune Mechanisms of Severe Equine Asthma-Current Understanding and What Is Missing. Animals (Basel) 2022 Mar 16;12(6).
    doi: 10.3390/ani12060744pubmed: 35327141google scholar: lookup
  3. Shankar J. Food Habit Associated Mycobiota Composition and Their Impact on Human Health. Front Nutr 2021;8:773577.
    doi: 10.3389/fnut.2021.773577pubmed: 34881282google scholar: lookup
  4. Rodríguez N, Whitfield-Cargile CM, Chamoun-Emanuelli AM, Hildreth E, Jordan W, Coleman MC. Nasopharyngeal bacterial and fungal microbiota in normal horses and horses with nasopharyngeal cicatrix syndrome. J Vet Intern Med 2021 Nov;35(6):2897-2911.
    doi: 10.1111/jvim.16307pubmed: 34783081google scholar: lookup
  5. Mainguy-Seers S, Lavoie JP. Glucocorticoid treatment in horses with asthma: A narrative review. J Vet Intern Med 2021 Jul;35(4):2045-2057.
    doi: 10.1111/jvim.16189pubmed: 34085342google scholar: lookup
  6. Mach N, Baranowski E, Nouvel LX, Citti C. The Airway Pathobiome in Complex Respiratory Diseases: A Perspective in Domestic Animals. Front Cell Infect Microbiol 2021;11:583600.
    doi: 10.3389/fcimb.2021.583600pubmed: 34055660google scholar: lookup
  7. Yang Y, Liu Y, Wang R, Liu J, Cui M, Duan Z. Explore impact of inhaled glucocorticoids/β₂-agonists on the oropharyngeal microbiota in asthma patients impact of GCs/β₂-agonists on oropharyngeal microbiota in asthma. Medicine (Baltimore) 2025 Dec 5;104(49):e46483.
    doi: 10.1097/MD.0000000000046483pubmed: 41367002google scholar: lookup
  8. Holley L, Creasey HN, Bedenice D, Reed S, Romualdo da Silva DR, Trautwein V, Mazan M, Widmer G. Nebulization of 2% lidocaine has no detectable impact on the healthy equine respiratory microbiota. PLoS One 2025;20(1):e0316079.
    doi: 10.1371/journal.pone.0316079pubmed: 39854381google scholar: lookup
  9. Dély S, Gerber V, Peters LM, Sage SE. Association between equine asthma and fungal elements in the tracheal wash: An environment-matched case-control study. PLoS One 2024;19(9):e0309835.
    doi: 10.1371/journal.pone.0309835pubmed: 39240830google scholar: lookup
  10. Mańkowska A, Witkowska D. The Most Common Environmental Risk Factors for Equine Asthma-A Narrative Review. Animals (Basel) 2024 Jul 14;14(14).
    doi: 10.3390/ani14142062pubmed: 39061524google scholar: lookup
  11. Mach N. The forecasting power of the mucin-microbiome interplay in livestock respiratory diseases. Vet Q 2024 Dec;44(1):1-18.
    doi: 10.1080/01652176.2024.2340003pubmed: 38606662google scholar: lookup
  12. Bond S, Léguillette R. A CONSORT-guided, randomized controlled clinical trial of nebulized administration of dexamethasone and saline on lower airway cytokine mRNA expression in horses with moderate asthma. J Vet Intern Med 2024 Mar-Apr;38(2):1214-1223.
    doi: 10.1111/jvim.16983pubmed: 38205666google scholar: lookup
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