Effects of phenylbutazone and oxyphenbutazone on basic drug detection in high performance thin layer chromatographic systems.
Abstract: Interference or 'masking' in thin layer chromatography occurs when the presence of one drug on a thin layer plate physically obscures or interferes with the detection of another drug. We investigated the ability of phenylbutazone and oxyphenbutazone to mask or interfere with the detection by high performance thin layer chromatography (HPTLC) of basic drugs used illegally in horse racing. Of fifty-five basic drugs called 'positive' since 1981 by laboratories affiliated with the Association of Official Racing Chemists (AORC), forty did not comigrate with phenylbutazone or oxyphenbutazone and could not, therefore, be masked. When 75 micrograms/ml of oxyphenbutazone was spiked into urine samples, subjected to an extraction procedure for basic drugs, and then run in our routine HPTLC systems, no 'spots' due to oxyphenbutazone appeared. 'Masking' by oxyphenbutazone, therefore, did not and could not occur in our test systems. When phenylbutazone at a concentration of 30 micrograms/ml was spiked into urine samples and run in the routine HPTLC system, phenylbutazone spots were visible under ultraviolet light and after certain specific oversprays were used to visualize basic drugs. These spots, however, did not interfere with routine thin layer testing for basic drugs. It was concluded that phenylbutazone and oxyphenbutazone had no significant ability to interfere with detection of the parent forms of these basic drugs under the conditions described in these experiments.
Publication Date: 1985-06-01 PubMed ID: 2862290DOI: 10.1111/j.1365-2885.1985.tb00942.xGoogle Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research paper assesses the potential of two drugs, phenylbutazone and oxyphenbutazone, to mask the detection of illegally used basic drugs in horse racing through high performance thin layer chromatography (HPTLC). It concludes that these two substances do not significantly obscure the identification of said illegal substances under tested conditions.
Understanding the Concept
- The study focused on the concept of ‘masking’ in thin layer chromatography. This is a phenomenon whereby the presence of one substance in a sample can either physically obscure or intervene with the detection of another substance.
- Phenylbutazone and oxyphenbutazone are drugs known for their anti-inflammatory effects. They were examined in this study to determine their potential on masking the detection of certain drugs, typically abused in horse racing.
Methodology and Findings
- The study was primarily concerned with fifty-five basic drugs, deemed ‘positive’ since 1981 by the Association of Official Racing Chemists (AORC)-connected labs. Out of these, forty drugs did not comigrate with phenylbutazone or oxyphenbutazone so could not be masked by them.
- Tests were conducted by spiking urine samples with oxyphenbutazone (75 micrograms/ml) and phenylbutazone (30 micrograms/ml), extracting the basic drugs from these samples, and then running these mixtures in routine HPTLC systems.
- The results showed that no spots due to oxyphenbutazone appeared during testing. This means that ‘masking’ by oxyphenbutazone did not and could not occur under these conditions. On the other hand, spots due to phenylbutazone were visible under ultraviolet light and after certain specific oversprays were used to visualize basic drugs. However, these spots didn’t interfere with standard thin layer tests for basic drugs.
Conclusion
- In conclusion, this research demonstrated that the two drugs phenylbutazone and oxyphenbutazone do not have a significant capacity to obscure the detection of the illegal drugs tested in this study. Therefore, the use of these drugs should not interfere with the process of identifying illicit substances in horse racing through the high performance thin layer chromatography method.
Cite This Article
APA
Woods WE, Chay S, Houston T, Blake JW, Tobin T.
(1985).
Effects of phenylbutazone and oxyphenbutazone on basic drug detection in high performance thin layer chromatographic systems.
J Vet Pharmacol Ther, 8(2), 181-189.
https://doi.org/10.1111/j.1365-2885.1985.tb00942.x Publication
Researcher Affiliations
MeSH Terms
- Animals
- Central Nervous System Stimulants / urine
- Chromatography, Thin Layer / methods
- False Negative Reactions
- Horses
- Narcotics / urine
- Oxyphenbutazone / urine
- Pharmaceutical Preparations / urine
- Phenylbutazone / urine
- Solvents
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