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Home / Equine Research Bank / Cartilage / Effects of Platelet-Rich Plasma Composition on Anabolic and ...
Cartilage2012; 3(3); 245-254; doi: 10.1177/1947603511433181

Effects of Platelet-Rich Plasma Composition on Anabolic and Catabolic Activities in Equine Cartilage and Meniscal Explants.

Abstract: To evaluate the effects of single- and double-spin preparations of platelet-rich plasma (PRP) on anabolic and catabolic activities of cartilage and meniscal explants in vitro. Methods: Single- and double-spin PRP was prepared using laboratory processing or commercial kits. The cellular contents were quantified, and each PRP was mixed in equal quantities with cell culture medium and added to cartilage or meniscus explant cultures, with or without interleukin 1 β (IL-1β). Extracellular matrix synthesis was quantified over 24 hours via (35)S-sulfate and (3)H-proline incorporation, while gene expression of catabolic enzymes was evaluated using real-time PCR. Results: The platelet concentration in single-spin laboratory PRP was 59% higher than blood. Platelet and white blood cell concentrations in single-spin laboratory and kit PRP were not significantly different, while the double-spin kit resulted in approximately 2.5-fold higher platelet and approximately 400-fold higher white blood cell concentrations. In cartilage cultures without IL-1β, radiolabel incorporation in single-spin PRP cultures was significantly higher than in double-spin cultures. Similar results were obtained for (35)S-sulfate incorporation in meniscus cultures without IL-1β. In IL-1β, radiolabel incorporation was largely similar among all PRPs. After 24 hours of culture, ADAMTS-4 gene expression in cartilage was lowest for single-spin PRP, while expression in the double-spin kit was not significantly different from double-spin laboratory PRP in which platelets were concentrated 6-fold. Conclusions: This study suggests that single-spin PRP preparations may be the most advantageous for intra-articular applications and that double-spin systems should be considered with caution.
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Publication Date: 2012-07-01 PubMed ID: 26069637PubMed Central: PMC4297115DOI: 10.1177/1947603511433181Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study investigated the effects of two different processes of preparing platelet-rich plasma (PRP), single- and double-spin, on cartilage and meniscus explants. The results indicated that single-spin PRP preparations appeared to be more beneficial for intra-articular applications, and using double-spin systems might warrant caution.

Methods

  • The researchers prepared PRP using both single- and double-spin methods utilizing either laboratory processing or commercial kits.
  • The cellular content of each PRP was quantified.
  • An equal amount of each PRP with cell culture medium was added to the cartilage and meniscus explant cultures, with some cultures also treated with interleukin 1 β (IL-1β).
  • The synthesis of the extracellular matrix was quantified over 24 hours using (35)S-sulfate and (3)H-proline incorporations.
  • The gene expression of the catabolic enzymes was evaluated by real-time PCR.

Results

  • The platelet concentration in the single-spin laboratory PRP was found to be 59% higher than the blood concentration.
  • The concentrations of platelets and white blood cells in the single-spin laboratory and kit PRP were not significantly different. However, double-spin kits yielded about 2.5 times higher platelet and around 400 times higher white blood cell concentrations.
  • In cartilage cultures without IL-1β, higher radiolabel incorporation was observed in single-spin PRP cultures compared to double-spin cultures. The same was shown for (35)S-sulfate incorporation in meniscus cultures without IL-1β.
  • With IL-1β treatment, radiolabel incorporation was nearly identical among all PRPs.
  • After 24 hours of culture, the expression of a cartilage-damaging enzyme gene ADAMTS-4 was lowest for single-spin PRP. The gene expression with the double-spin kit was not significantly different from double-spin processed in the lab where the platelets were concentrated six-fold.

Conclusions

  • The researchers suggested that single-spin PRP preparations may be more advantageous for procedures involving the joint cavity based on their impact on the extracellular matrix synthesis and the ADAMTS-4 gene expression.
  • On the other hand, the study cautioned the use of double-spin systems due to their high platelet and white blood cell concentrations, hinting the possibility that they may not offer the same advantages as the single-spin preparations.

Cite This Article

APA
Kisiday JD, McIlwraith CW, Rodkey WG, Frisbie DD, Steadman JR. (2012). Effects of Platelet-Rich Plasma Composition on Anabolic and Catabolic Activities in Equine Cartilage and Meniscal Explants. Cartilage, 3(3), 245-254. https://doi.org/10.1177/1947603511433181

Publication

Cartilage
ISSN: 1947-6035
NlmUniqueID: 101518378
Country: United States
Language: English
Volume: 3
Issue: 3
Pages: 245-254

Researcher Affiliations

Kisiday, John D
  • Department of Clinical Science, Orthopaedic Research Center, Colorado State University, Fort Collins, CO, USA.
McIlwraith, C Wayne
  • Department of Clinical Science, Orthopaedic Research Center, Colorado State University, Fort Collins, CO, USA.
Rodkey, William G
  • Steadman Philippon Research Institute, Vail, CO, USA.
Frisbie, David D
  • Department of Clinical Science, Orthopaedic Research Center, Colorado State University, Fort Collins, CO, USA.
Steadman, J Richard
  • Steadman Philippon Research Institute, Vail, CO, USA.

Conflict of Interest Statement

Declaration of Conflicting Interests: The author(s) declared the following potential conflicts of interest with respect to the research, authorship, and/or publication of this article: Drs. McIlwraith and Frisbie are consultants for Arthrex, the company that manufactures the ACP kit used in this study.

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Citations

This article has been cited 18 times.
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