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Home / Equine Research Bank / Equine Vet J Suppl / Effects of repeated intra-articular administration of amikac...
Equine veterinary journal. Supplement2013; (43); 12-16; doi: 10.1111/j.2042-3306.2012.00637.x

Effects of repeated intra-articular administration of amikacin on serum amyloid A, total protein and nucleated cell count in synovial fluid from healthy horses.

Abstract: Serum amyloid A (SAA) in synovial fluid has recently been used as a marker for septic arthritis in horses but the effects of repeated intra-articular (IA) administration of amikacin on synovial SAA concentrations are unknown. Objective: To report the effect of repeated IA administration of amikacin on SAA, total protein (TP), nucleated cell count (NCC) and differential NCC in synovial fluid of healthy equine joints. Methods: A controlled, 2 period crossover study was performed on 5 clinically healthy horses. Each intercarpal joint received one of 2 treatments every 48 h for 5 consecutive times: arthrocentesis alone (control group) or arthrocentesis combined with IA administration of 500 mg of amikacin (treatment group). Clinical and lameness examinations were performed daily. Serum SAA and synovial SAA, TP, NCC and differential NCC were measured and statistically compared. Significance level was set at P < 0.05. Results: Horses remained healthy and nonlame throughout the study. Baseline values for all variables were not significantly different between groups. Values for TP in the treatment group were significantly higher than in the control group after the first sample (P < 0.05). In both groups NCC increased significantly (P < 0.05) after the first sample. No significant changes were identified in differential NCC. In both groups, all synovial and most serum SAA concentrations remained below the lower limit of quantification. Conclusions: Repeated IA administration of amikacin caused increased values of TP and NCC in synovial fluid, with some TP concentrations falling within the range reported for septic arthritis. In contrast, synovial SAA concentrations did not increase in either group. Conclusions: Synovial SAA could serve as a more reliable marker than TP and NCC when evaluating a joint previously sampled or treated with amikacin.
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Publication Date: 2013-03-02 PubMed ID: 23447871DOI: 10.1111/j.2042-3306.2012.00637.xGoogle Scholar: Lookup
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  • Controlled Clinical Trial
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article investigates the effects of applying amikacin, a type of antibiotic, directly into a joint of healthy horses and focuses on how it might impact SAA (a protein marker for inflammation), total protein, and nucleated cell count in the synovial fluid. The study suggests that synovial Serum Amyloid A (SAA) could be a more reliable marker than total protein and nucleated cell count when evaluating a horse’s joint that has been previously treated with amikacin.

Objectives and Methodology

  • The aim of this controlled, crossover study was to observe the impact of repeated intra-articular administration of amikacin on horses’ synovial fluid’s SAA, total protein, nucleated cell count and differential nucleated cell count.
  • The experiments were conducted on five clinically healthy horses, with each intercarpal joint receiving one of two treatments for five consecutive times. These treatments were either arthrocentesis alone in the control group or arthrocentesis combined with an intra-articular administration of 500mg of amikacin in the treatment group. Regular clinical and lameness examinations were performed on all horses daily.

Results and Findings

  • The horses remained healthy and nonlame throughout the study. There were no significant differences in baseline values for all variables between the two groups.
  • However, values for total protein in the treatment group were significantly higher than in the control group after the first sample.
  • Nucleated cell count also increased significantly in both groups after the first sample but there were no significant changes identified in the differential nucleated cell count.
  • All synovial and most serum SAA concentrations remained below the lower limit of quantification in both groups which implies that administration of amikacin did not elevate SAA levels.

Conclusions

  • The study concluded that repeated intra-articular administration of amikacin caused increased values of total protein and nucleated cell count in the synovial fluid but did not affect the levels of synovial SAA levels, with some total protein concentrations even falling within the range reported for septic arthritis.
  • The fact that synovial SAA concentrations did not increase in either group implies that synovial SAA could potentially be a more reliable marker than total protein and nucleated cell count when evaluating a joint in a horse that has been previously sampled or treated with amikacin.

Cite This Article

APA
Sanchez Teran AF, Rubio-Martinez LM, Villarino NF, Sanz MG. (2013). Effects of repeated intra-articular administration of amikacin on serum amyloid A, total protein and nucleated cell count in synovial fluid from healthy horses. Equine Vet J Suppl(43), 12-16. https://doi.org/10.1111/j.2042-3306.2012.00637.x

Publication

Equine veterinary journal. Supplement
NlmUniqueID: 9614088
Country: United States
Language: English
Issue: 43
Pages: 12-16

Researcher Affiliations

Sanchez Teran, A F
  • Department of Companion Animal Clinical Studies, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa.
Rubio-Martinez, L M
    Villarino, N F
      Sanz, M G

        MeSH Terms

        • Amikacin / administration & dosage
        • Amikacin / adverse effects
        • Animals
        • Cross-Over Studies
        • Female
        • Horse Diseases / chemically induced
        • Horses
        • Injections, Intra-Articular
        • Joint Diseases / chemically induced
        • Joint Diseases / veterinary
        • Proteins / chemistry
        • Proteins / metabolism
        • Serum Amyloid A Protein / chemistry
        • Serum Amyloid A Protein / metabolism
        • Synovial Fluid / chemistry
        • Synovial Fluid / cytology

        Citations

        This article has been cited 17 times.
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