Abstract: Stress associated with road transport is believed to be a significant contributor to the pathogenesis of post transport respiratory disease in horses. To determine the effects of road transport on pulmonary function, pulmonary aerosol clearance rates were measured in 4 horses 24 h before, and immediately after, 24 h of road transport by delivering aerosolised 99mtechnetium-labelled diethylenetriaminepentacetate (99mTc-DTPA) to the lungs and monitoring its washout. Each horse was transported twice, once while the trailer was equipped with a leaf-spring suspension and bias-ply tyres (trailer's original equipment, smooth ride) and once while the trailer was equipped with a torsion-bar suspension and normal pressure radial tyres (rough ride) in order to generate different ride characteristics. Before transport, blood was drawn from each horse for haematology and measurement of serum cortisol concentration; 24 h rates of hay and water intake and faecal output were recorded for each horse. Horses were then transported, 2 at a time, over a 128 km circular route of predominantly rural freeways at a constant speed of 72 km/h for 24 h. Horses were rested by stopping the trailer every 3.75 h for 0.25 h. During transport, heart rates (continuous 1 min averages), rates of hay and water intake and rates of faecal output were measured. Ammonia (NH3) and carbon monoxide (CO) concentrations were measured within the trailer and temperatures (wet bulb [WB], dry bulb [DB] and black globe [BG]) within the trailer were recorded each minute. Immediately after each experiment blood was drawn for haematology and measurement of pulmonary aerosol clearance rates were measured. For control studies, horses were housed in their stalls while heart rates were measured for 24 h. Slopes calculated from the 99mTc-DTPA clearance curves for pretransport horses were not significantly different from post transport clearance slopes. Pretransport mean 99mTc-DTPA clearance half-lives (T50, left lung mean +/- s.d. 41.7 +/- 15.8 min, right lung 44.6 +/- 19.1 min) were not significantly different from post transport T50 (left lung 53.5 +/- 14.0 min, right lung 52.0 +/- 11.6 min). Heart rates during transport were not affected by suspension type or trip order (the horse's first or second transport experiment) and were not significantly different from stall controls after the first 120 min of the experiment. Horses had increased red blood cell count, packed cell volume, haemoglobin, plasma protein and cortisol concentrations, and decreased body weights immediately post transport, indicating slight dehydration. Water and hay intake rates were significantly lower during transport than pretransport. Temperatures within the trailer were highest in the midafternoon and lowest in the early morning hours, but all temperatures measured in the trailer were within the comfort zone for large homeotherms. Ammonia and CO concentrations in the trailer during the transport period were within acceptable limits for human exposure. However, respirable articulates in the atmosphere were elevated above safe concentrations for human exposure.
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The research examines how road transport can contribute to stress in horses, focusing on its effects on pulmonary function and various other physiological parameters. The authors of the study found some changes in the horses’ physiological responses following transport but did not observe significant differences in pulmonary function before and after transport.
Research Methodology
The researchers examined the effects of road transport on horses by studying four horses both before and after a 24-hour period of transportation. The experiment was conducted twice, once with a smooth ride setup (leaf-spring suspension and bias-ply tyres) and once with a rough ride setup (torsion-bar suspension and regular pressure radial tyres), to test different ride characteristics.
Several measurements were taken before, during and after the transport including heart rates, rates of hay and water intake, faecal output, and serum cortisol concentration. The trailer’s temperature and levels of ammonia and carbon monoxide were also monitored.
The horses were driven for 24 hours on a predominantly rural freeway route, with the trailer stopping every 3.75 hours for a 25-minute rest period.
As a control, measurements were also taken for the horses over a 24-hour period while they were housed in their stalls.
Main Findings
The study found no significant difference in the pulmonary aerosol washout rates of the horses before and after transport, indicating that transport did not significantly affect their pulmonary function.
Heart rates during transport were unaffected by the type of suspension in the trailer or whether it was the horse’s first or second transport experiment. They were also not significantly different from the control measurements in the stall after the first 120 minutes of the experiment.
However, increases were observed in red blood cell count, packed cell volume, haemoglobin, plasma protein and cortisol concentrations immediately after transport, which could indicate mild dehydration. Body weight was seen to decrease post transport.
Water and hay intake rates were significantly lower during transport, contributing to the indication of mild dehydration.
The temperatures within the trailer varied but generally stayed within the comfort range for large homeotherms. Levels of ammonia and carbon monoxide within the trailer during the transport period were within acceptable limits for human exposure. However, respirable particulates in the atmosphere were observed at levels above safe concentrations for humans, potentially indicating a poor quality of air within the trailer.
Concluding Remarks
The study suggests that while there may be some physiological changes in horses during road transport, their pulmonary function and heart rate remain largely unchanged. However, the increased stress markers and decreased body weight may indicate a slight level of dehydration following road transport.
The findings also indicate that even when temperature control and harmful gas levels are maintained within acceptable limits, the air quality in terms of particle content may need improvement during long periods of transport to ensure better conditions for the horses.
Cite This Article
APA
Smith BL, Jones JH, Hornof WJ, Miles JA, Longworth KE, Willits NH.
(1996).
Effects of road transport on indices of stress in horses.
Equine Vet J, 28(6), 446-454.
https://doi.org/10.1111/j.2042-3306.1996.tb01616.x
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