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BMC veterinary research2015; 11; 60; doi: 10.1186/s12917-015-0370-4

Effects of sodium citrate and acid citrate dextrose solutions on cell counts and growth factor release from equine pure-platelet rich plasma and pure-platelet rich gel.

Abstract: There is a lack information on the effects of the most commonly used anticoagulants for equine platelet rich plasmas (PRPs) elaboration on cell counts and growth factor release from platelet rich gels (PRGs). The aims of this study were 1) to compare the effects of the anticoagulants sodium citrate (SC), acid citrate dextrose solution A (ACD-A) and ACD-B on platelet (PLT), leukocyte (WBC) and on some parameters associated to platelet activation including mean platelet volume (MPV) and platelet distribution width (PDW) between whole blood, pure PRP (P-PRP) and platelet-poor plasma (PPP); 2) to compare transforming growth factor beta 1 (TGF-β(1)) and platelet-derived growth factor isoform BB (PDGF-BB) concentrations in supernatants from pure PRG (P-PRG), platelet-poor gel (PPG), P-PRP lysate (positive control) and plasma (negative control); 3) to establish the possible correlations between all the studied cellular and molecular parameters. Results: In all cases the three anticoagulants produced P-PRPs with significantly higher PLT counts compared with whole blood and PPP. The concentrations of WBCs were similar between P-PRP and whole blood, but significantly lower in PPP. The type of anticoagulant did not significantly affect the cell counts for each blood component. The anticoagulants also did not affect the MPV and PDW parameters. Independently of the anticoagulant used, all blood components presented significantly different concentrations of PDGF-BB and TGF-β(1). The highest growth factor (GF) concentrations were observed from P-PRP lysates, followed by PRG supernatants, PPP lysates, PPG supernatants and plasma. Significant correlations were observed between PLT and WBC counts (ρ = 0.80), PLT count and TGF-β(1) concentration (ρ = 0.85), PLT count and PDGF-BB concentration (ρ = 0.80) and PDGF-BB and TGF-β(1) concentrations (ρ = 0.75). The type of anticoagulant was not correlated with any of the variables evaluated. Conclusions: The anticoagulants did not significantly influence cell counts or GF concentrations in equine PRP. However, ACD-B was apparently the worst anticoagulant evaluated. It is necessary to perform additional research to determine the effect of anticoagulants on the kinetics of GF elution from P-PRG.
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Publication Date: 2015-03-14 PubMed ID: 25889052PubMed Central: PMC4364319DOI: 10.1186/s12917-015-0370-4Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research paper examines how the use of different anticoagulants affects the levels of cell counts and the release of growth factors from equine platelet rich plasma and gels. The study found that the type of anticoagulant used did not significantly influence these attributes, though did suggest further research to explore the effects of anticoagulants on the processes within these biological materials.

Objectives

  • The research aimed to observe how different anticoagulants like sodium citrate (SC), acid citrate dextrose solution A (ACD-A) and ACD-B impacted cellular densities of platelet (PLT), leukocyte (WBC) and some parameters linked to platelet activation.
  • The research also intended to compare the concentrations of specific growth factors (TGF-β(1) and PDGF-BB) in various biological materials processed with different anticoagulants.
  • Lastly, the study intended to find out if there were correlations between the cellular and molecular parameters they were investigating.

Findings

  • All three anticoagulants resulted in platelet rich plasmas that had significantly higher platelet counts compared with whole blood and platelet-poor plasma.
  • The concentrations of white blood cells were similar between the platelet rich plasma and whole blood, but significantly lower in platelet poor plasma.
  • No significant variation was found in these counts based on the type of anticoagulant.
  • The anticoagulants also did not result in significant shifts in Mean Platelet Volume (MPV) and Platelet Distribution Width (PDW).
  • In terms of growth factor concentration, the research found that all tested blood components presented significantly different concentrations of PDGF-BB and TGF-β(1), with the highest growth factor concentrations observed in Platelet Rich Plasma lysates.
  • The study also found strong correlations between platelet and white blood cell counts, and between platelet count and growth factor concentrations.

Conclusion

  • The research concludes that the type of anticoagulant used does not significantly influence cell counts or growth factor concentrations in equine Platelet Rich Plasma (PRP).
  • Nevertheless, ACD-B was found to be the least effective of the anticoagulants tested for this use.
  • The researchers suggest that additional investigations are needed to understand the effects of anticoagulants on the kinetics of growth factor elution from platelet rich gels.

Cite This Article

APA
Giraldo CE, Álvarez ME, Carmona JU. (2015). Effects of sodium citrate and acid citrate dextrose solutions on cell counts and growth factor release from equine pure-platelet rich plasma and pure-platelet rich gel. BMC Vet Res, 11, 60. https://doi.org/10.1186/s12917-015-0370-4

Publication

BMC veterinary research
ISSN: 1746-6148
NlmUniqueID: 101249759
Country: England
Language: English
Volume: 11
Pages: 60
PII: 60

Researcher Affiliations

Giraldo, Carlos E
  • Grupo de Investigación Terapia Regenerativa, Departamento de Salud Animal, Universidad de Caldas, Manizales, Colombia. cgiraldo@ucaldas.edu.co.
Álvarez, María E
  • Grupo de Investigación Terapia Regenerativa, Departamento de Salud Animal, Universidad de Caldas, Manizales, Colombia. maria.alvarez_l@ucaldas.edu.co.
Carmona, Jorge U
  • Grupo de Investigación Terapia Regenerativa, Departamento de Salud Animal, Universidad de Caldas, Manizales, Colombia. carmona@ucaldas.edu.co.

MeSH Terms

  • Animals
  • Anticoagulants / pharmacology
  • Becaplermin
  • Blood Cell Count / veterinary
  • Blood Platelets / chemistry
  • Blood Platelets / drug effects
  • Blood Platelets / metabolism
  • Citrates / pharmacology
  • Citric Acid / pharmacology
  • Enzyme-Linked Immunosorbent Assay / veterinary
  • Glucose / analogs & derivatives
  • Glucose / pharmacology
  • Horses / blood
  • Horses / physiology
  • Leukocyte Count / veterinary
  • Male
  • Platelet Activation / drug effects
  • Platelet Count / veterinary
  • Platelet-Rich Plasma / chemistry
  • Platelet-Rich Plasma / cytology
  • Platelet-Rich Plasma / drug effects
  • Proto-Oncogene Proteins c-sis / analysis
  • Sodium Citrate
  • Transforming Growth Factor beta1 / analysis

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Citations

This article has been cited 18 times.
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