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Effects of sodium hyaluronate and methylprednisolone acetate on proteoglycan metabolism in equine articular chondrocytes treated with interleukin-1.
Abstract: To determine the effects of sodium hyaluronate (HA) in combination with methylprednisolone acetate (MPA) on interleukin-1 (IL-1)-induced inflammation in equine articular cartilage pellets. Methods: Chondrocytes collected from 7 horses euthanatized for problems unrelated to the musculoskeletal system. Methods: Chondrocyte pellets were treated with medium (negative control); medium containing IL-1 (positive control); or medium containing IL-1 with MPA only (0.05 or 0.5 mg/mL), HA only (0.2 or 2 mg/mL), or MPA (0.05 or 0.5 mg/mL) and HA (0.2 or 2 mg/mL) in combination. Proteoglycan (PG) synthesis was determined by incorporation of sulfur 35-labeled sodium sulfate into PGs. Glycosaminoglycan (GAG) content of the media and the pellets and total pellet DNA content were determined. Results: Methylprednisolone acetate at 0.5 mg/mL caused an increase in PG synthesis, whereas HA had no effect alone. The combination of MPA, both 0.05 mg/mL and 0.5 mg/mL, with HA at 2 mg/mL increased PG synthesis, compared with IL-1-treated control. All treatment groups containing the high concentration of MPA (0.5 mg/mL) and the high concentration of HA (2.0 mg/mL) had pellets with increased GAG content. The addition of HA caused an increase in total GAG content in the media, regardless of MPA treatment. Cyclooxygenase-2 mRNA and aggrecan mRNA expression was significantly reduced with MPA treatment. Total pellet DNA content was unchanged by any treatment. Conclusions: Our results indicate that MPA in combination with HA has beneficial effects on PG metabolism of IL-1-treated equine chondrocytes.
Publication Date: 2006-12-06 PubMed ID: 17144797DOI: 10.2460/ajvr.67.12.1980Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Comparative Study
- Journal Article
- Research Support
- U.S. Gov't
- Non-P.H.S.
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The study investigates how sodium hyaluronate (HA) and methylprednisolone acetate (MPA) affect inflammation-induced degradation of articular cartilage in horses. The researchers concluded that MPA and HA combined can positively influence the proteoglycan metabolism in inflamed equine articular chondrocytes.
Methods
- The study uses chondrocyte cells collected from 7 horses that were euthanized due to non-musculoskeletal related problems.
- These chondrocytes were cultured into pellets and subjected to varying treatments. The negative control group was treated with a substance carrier or medium. The positive control was introduced to medium containing inflammatory cytokine interleukin-1 (IL-1).
- The remaining groups were treated with medium containing single or combined doses of MPA and HA. These also had IL-1 to mimic an inflamed environment.
- To measure proteoglycan (PG) synthesis, researchers tracked the incorporation of sulfur 35-labeled sodium sulfate into PGs.
- The glycosaminoglycan (GAG) content was determined in both the culture medium and the cell pellets, and total pellet DNA content was also recorded.
Results
- MPA at a concentration of 0.5 mg/mL enhanced PG synthesis. HA on its own did not have a significant effect on this metric.
- The combined treatment of MPA (at both 0.05 mg/mL and 0.5 mg/mL) with HA at 2 mg/mL increased PG synthesis compared to the IL-1 treated control group.
- All treatment groups that were introduced to the higher concentrations of MPA (0.5 mg/mL) and HA (2.0 mg/mL) had cell pellets with increased GAG content.
- Adding HA to the medium led to an increase in the total GAG content in the medium, irrespective of whether MPA was present.
- Cyclooxygenase-2 mRNA and aggrecan mRNA expression was significantly reduced with MPA treatment.
- Total pellet DNA content did not appear to be significantly affected by any of the treatments.
Conclusions
- The results suggest that the combination of MPA and HA has beneficial effects on the metabolism of proteoglycans in inflamed equine articular chondrocytes, contributing to potential therapeutic applications in managing cartilage-related ailments in horses.
Cite This Article
APA
Yates AC, Stewart AA, Byron CR, Pondenis HC, Kaufmann KM, Constable PD.
(2006).
Effects of sodium hyaluronate and methylprednisolone acetate on proteoglycan metabolism in equine articular chondrocytes treated with interleukin-1.
Am J Vet Res, 67(12), 1980-1986.
https://doi.org/10.2460/ajvr.67.12.1980 Publication
Researcher Affiliations
- Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois, Urbana, IL 61802.
MeSH Terms
- Adjuvants, Immunologic / administration & dosage
- Adjuvants, Immunologic / pharmacology
- Animals
- Anti-Inflammatory Agents / administration & dosage
- Anti-Inflammatory Agents / pharmacology
- Cells, Cultured
- Chondrocytes / cytology
- Chondrocytes / drug effects
- Chondrocytes / metabolism
- Horses / metabolism
- Hyaluronic Acid / administration & dosage
- Hyaluronic Acid / pharmacology
- Inflammation / drug therapy
- Interleukin-1 / toxicity
- Methylprednisolone / administration & dosage
- Methylprednisolone / analogs & derivatives
- Methylprednisolone / pharmacology
- Methylprednisolone Acetate
- Proteoglycans / analysis
- Proteoglycans / drug effects
- Proteoglycans / metabolism
- Sulfur Radioisotopes / analysis
Citations
This article has been cited 7 times.- Quam VG, Belacic ZA, Long S, Rice HC, Dhar MS, Durgam S. Equine bone marrow MSC-derived extracellular vesicles mitigate the inflammatory effects of interleukin-1β on navicular tissues in vitro. Equine Vet J 2025 Jan;57(1):232-242.
- Sullivan SN, Altmann NN, Brokken MT, Durgam SS. In vitro Effects of Methylprednisolone Acetate on Equine Deep Digital Flexor Tendon-Derived Cells. Front Vet Sci 2020;7:486.
- Kongdang P, Chokchaitaweesuk C, Tangyuenyong S, Ongchai S. Proinflammatory Effects of IL-1β Combined with IL-17A Promoted Cartilage Degradation and Suppressed Genes Associated with Cartilage Matrix Synthesis In Vitro. Molecules 2019 Oct 13;24(20).
- Castro Martins M, Peffers MJ, Lee K, Rubio-Martinez LM. Effects of stanozolol on normal and IL-1β-stimulated equine chondrocytes in vitro. BMC Vet Res 2018 Mar 20;14(1):103.
- Euppayo T, Punyapornwithaya V, Chomdej S, Ongchai S, Nganvongpanit K. Effects of hyaluronic acid combined with anti-inflammatory drugs compared with hyaluronic acid alone, in clinical trials and experiments in osteoarthritis: a systematic review and meta-analysis. BMC Musculoskelet Disord 2017 Sep 6;18(1):387.
- Wei G, Zhou C, Wang G, Fan L, Wang K, Li X. Keratinocyte Growth Factor Combined with a Sodium Hyaluronate Gel Inhibits Postoperative Intra-Abdominal Adhesions. Int J Mol Sci 2016 Sep 22;17(10).
- Euppayo T, Siengdee P, Buddhachat K, Pradit W, Chomdej S, Ongchai S, Nganvongpanit K. In vitro effects of triamcinolone acetonide and in combination with hyaluronan on canine normal and spontaneous osteoarthritis articular cartilage. In Vitro Cell Dev Biol Anim 2016 Aug;52(7):723-35.
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