Effects of sodium hyaluronate and triamcinolone acetonide on glucosaminoglycan metabolism in equine articular chondrocytes treated with interleukin-1.
Abstract: OBJECTIVE-To determine whether the effects of a high-molecular-weight sodium hyaluronate alone or in combination with triamcinolone acetonide can mitigate chondrocyte glyocosaminoglycan (GAG) catabolism caused by interleukin (IL)-1 administration. SAMPLE POPULATION-Chondrocytes collected from metacarpophalangeal joints of 10 horses euthanized for reasons unrelated to joint disease. PROCEDURES-Chondrocyte pellets were treated with medium (negative control), medium containing IL-1 only (positive control), or medium containing IL-1 with hyaluronic acid only (0.5 or 2.0 mg/mL), triamcinolone acetonide only (0.06 or 0.6 mg/mL), or hyaluronic acid (0.5 or 2.0 mg/mL) and triamcinolone acetonide (0.06 or 0.6 mg/mL) in combination. Chondrocyte pellets were assayed for newly synthesized GAG, total GAG content, total DNA content, and mRNA for collagen type II, aggrecan, and cyclooxygenase (COX)-2. RESULTS-High-concentration hyaluronic acid increased GAG synthesis, whereas high-concentration triamcinolone acetonide decreased loss of GAG into the medium. High concentrations of hyaluronic acid and triamcinolone acetonide increased total GAG content. There was no change in DNA content with either treatment. Triamcinolone acetonide reduced COX-2 mRNA as well as aggrecan and collagen type II expression. Treatment with hyaluronic acid had no effect on mRNA for COX-2, aggrecan, or collagen type II. CONCLUSIONS AND CLINICAL RELEVANCE-Results indicated that high concentrations of hyaluronic acid or triamcinolone acetonide alone or in combination mitigated effects of IL-1 administration on GAG catabolism of equine chondrocytes.
Publication Date: 2009-12-03 PubMed ID: 19951121DOI: 10.2460/ajvr.70.12.1494Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research investigated if high-molecular-weight sodium hyaluronate, alone or in partnership with triamcinolone acetonide, could counteract the negative effects of interleukin-1 on the metabolism of glycosaminoglycan in horse joint cells.
Research Objectives
This study aimed to understand the impact of sodium hyaluronate and triamcinolone acetonide on the metabolism of glycosaminoglycan (GAG) in equine articular chondrocytes negatively affected by interleukin (IL)-1. The researchers wanted to ascertain if these substances could alleviate the harm IL-1 inflicts on chondrocytes and promote healthy GAG metabolism.
Methodology
- The research involved chondrocytes obtained from the metacarpophalangeal joints of 10 horses, which were euthanized for unrelated reasons.
- A variety of treatments were used on the chondrocyte pellets: some received only medium (negative control), others had medium with IL-1 (positive control), and other groups also had the addition of hyaluronic acid (at two different concentrations), triamcinolone acetonide (at two different concentrations), or a combination of either concentration of hyaluronic acid and either concentration of triamcinolone acetonide.
- After these treatments, the chondrocyte pellets were tested for newly formed GAG, total GAG content, total DNA content, and the mRNA for three elements: aggrecan, collagen type II, and cyclooxygenase (COX)-2.
Results
- The application of high-concentration hyaluronic acid boosted GAG synthesis while high-concentration triamcinolone acetonide reduced GAG loss into the medium. Both elements increased total GAG content when used at high concentrations.
- The treatments did not affect the DNA content.
- Triamcinolone acetonide lessened the expression of COX-2 mRNA, aggrecan, and collagen type II. Hyaluronic acid did not have any effect on mRNA for COX-2, aggrecan, or collagen type II.
Conclusions and Clinical Relevance
The research findings highlight the potential of high-concentration hyaluronic acid and triamcinolone acetonide on their own, or together, to counteract the side effects of IL-1 administration on GAG metabolism in equine chondrocytes. The results open up possibilities for further study and potential therapeutic applications to maintain joint health in horses.
Cite This Article
APA
Schaefer EC, Stewart AA, Durgam SS, Byron CR, Stewart MC.
(2009).
Effects of sodium hyaluronate and triamcinolone acetonide on glucosaminoglycan metabolism in equine articular chondrocytes treated with interleukin-1.
Am J Vet Res, 70(12), 1494-1501.
https://doi.org/10.2460/ajvr.70.12.1494 Publication
Researcher Affiliations
- Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois, Urbana, IL 61802, USA.
MeSH Terms
- Adjuvants, Immunologic / administration & dosage
- Adjuvants, Immunologic / pharmacology
- Animals
- Anti-Inflammatory Agents / administration & dosage
- Anti-Inflammatory Agents / pharmacology
- Cells, Cultured
- Chondrocytes / drug effects
- Chondrocytes / metabolism
- Dose-Response Relationship, Drug
- Glycosaminoglycans / metabolism
- Horses
- Hyaluronic Acid / pharmacology
- Interleukin-1 / pharmacology
- Triamcinolone Acetonide / pharmacology
Citations
This article has been cited 7 times.- Bauer C, Moser LB, Jeyakumar V, Niculescu-Morzsa E, Kern D, Nehrer S. Increased Chondroprotective Effect of Combining Hyaluronic Acid with a Glucocorticoid Compared to Separate Administration on Cytokine-Treated Osteoarthritic Chondrocytes in a 2D Culture. Biomedicines 2022 Jul 18;10(7).
- Oh SH, Sung WS, Oh SH, Jo CH. Comparative analysis of intra-articular injection of steroid and/or sodium hyaluronate in adhesive capsulitis: prospective, double-blind, randomized, placebo-controlled study. JSES Int 2021 Nov;5(6):1091-1104.
- Velloso Alvarez A, Boone LH, Pondugula SR, Caldwell F, Wooldridge AA. Effects of Autologous Conditioned Serum, Autologous Protein Solution, and Triamcinolone on Inflammatory and Catabolic Gene Expression in Equine Cartilage and Synovial Explants Treated With IL-1β in Co-culture. Front Vet Sci 2020;7:323.
- Castro Martins M, Peffers MJ, Lee K, Rubio-Martinez LM. Effects of stanozolol on normal and IL-1β-stimulated equine chondrocytes in vitro. BMC Vet Res 2018 Mar 20;14(1):103.
- Euppayo T, Punyapornwithaya V, Chomdej S, Ongchai S, Nganvongpanit K. Effects of hyaluronic acid combined with anti-inflammatory drugs compared with hyaluronic acid alone, in clinical trials and experiments in osteoarthritis: a systematic review and meta-analysis. BMC Musculoskelet Disord 2017 Sep 6;18(1):387.
- Avenoso A, D'Ascola A, Scuruchi M, Mandraffino G, Calatroni A, Saitta A, Campo S, Campo GM. Hyaluronan in the experimental injury of the cartilage: biochemical action and protective effects. Inflamm Res 2018 Jan;67(1):5-20.
- Euppayo T, Siengdee P, Buddhachat K, Pradit W, Chomdej S, Ongchai S, Nganvongpanit K. In vitro effects of triamcinolone acetonide and in combination with hyaluronan on canine normal and spontaneous osteoarthritis articular cartilage. In Vitro Cell Dev Biol Anim 2016 Aug;52(7):723-35.
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