Effects of syringe type and storage conditions on results of equine blood gas and acid-base analysis.
Abstract: To determine effects of syringe type and storage conditions on blood gas and acid-base values for equine blood samples. Methods: Blood samples obtained from 8 healthy horses. Methods: Heparinized jugular venous blood was equilibrated via a tonometer at 37°C with 12% O(2) and 5% CO(2). Aliquots (3 mL) of tonometer-equilibrated blood were collected in random order by use of a glass syringe (GS), general-purpose polypropylene syringe (GPPS), or polypropylene syringe designed for blood gas analysis (PSBGA) and stored in ice water (0°C) or at room temperature (22°C) for 0, 5, 15, 30, 60, or 120 minutes. Blood pH was measured, and blood gas analysis was performed; data were analyzed by use of multivariable regression analysis. Results: Blood Po(2) remained constant for the reference method (GS stored at 0°C) but decreased linearly at a rate of 7.3 mm Hg/h when stored in a GS at 22°C. In contrast, Po(2) increased when blood was stored at 0°C in a GPPS and PSBGA or at 22°C in a GPPS; however, Po(2) did not change when blood was stored at 22°C in a PSBGA. Calculated values for plasma concentration of HCO(3) and total CO(2) concentration remained constant in the 3 syringe types when blood was stored at 22°C for 2 hours but increased when blood was stored in a GS or GPPS at 0°C. Conclusions: Blood samples for blood gas and acid-base analysis should be collected into a GS and stored at 0°C or collected into a PSBGA and stored at room temperature.
Publication Date: 2012-06-29 PubMed ID: 22738049DOI: 10.2460/ajvr.73.7.979Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This study investigated how the type of syringe and storage conditions can affect the results of blood gas and acid-base analysis in equine blood samples. The researchers concluded that blood samples should be collected either with a glass syringe (GS) and stored at 0°C or using a polypropylene syringe designed for blood gas analysis (PSBGA) while storing it at room temperature.
Methods
- The research was performed on blood samples gathered from eight healthy horses.
- The samples were collected using three different types of syringes: a glass syringe (GS), a general-purpose polypropylene syringe (GPPS), and a polypropylene syringe specifically designed for blood gas analysis (PSBGA).
- Heparin-treated blood was equilibrated with 12% O2 and 5% CO2 using a tonometer at 37°C.
- After being tonometer-equilibrated, aliquots (3 mL) of each blood sample were collected in random order and stored in one of two conditions: ice water (0°C) or room temperature (22°C).
- Storage times varied, ranging from immediate analysis to storage for two hours.
- Blood pH was measured and blood gas analysis was performed, with data being examined using multivariable regression analysis.
Results
- The oxygen partial pressure (Po(2)) in blood remained stable for samples stored at 0°C in the GS (the reference method).
- However, when stored in a GS at 22°C, the Po(2) decreased linearly at a rate of 7.3 mm Hg/hour.
- On the other hand, the Po(2) increased in the GPPS and PSBGA when the blood was stored at 0°C, and when using a GPPS at 22°C, but remained stable when stored in a PSBGA at 22°C.
- Calculated plasma concentrations of bicarbonate (HCO(3)) and total CO(2) remained stable across all syringe types if the blood was stored at 22°C for two hours, but they increased if stored at 0°C in a GS or GPPS.
Conclusions
- The results indicate that for accurate blood gas and acid-base analysis, samples should be collected in a GS and stored at 0°C, or collected in a PSBGA and stored at room temperature.
- The type of syringe used and storage conditions can significantly alter the results of blood gas and acid-base values in equine blood samples.
Cite This Article
APA
Kennedy SA, Constable PD, Sen I, Couëtil L.
(2012).
Effects of syringe type and storage conditions on results of equine blood gas and acid-base analysis.
Am J Vet Res, 73(7), 979-987.
https://doi.org/10.2460/ajvr.73.7.979 Publication
Researcher Affiliations
- Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Purdue University, West Lafayette, IN 47907, USA.
MeSH Terms
- Animals
- Blood Gas Analysis / methods
- Blood Gas Analysis / veterinary
- Female
- Horses / blood
- Male
- Multivariate Analysis
- Random Allocation
- Regression Analysis
- Specimen Handling / methods
- Specimen Handling / veterinary
- Syringes / veterinary
Citations
This article has been cited 1 times.- Sandersen C, Dmitrovic P, Dupont J, Cesarini C, Guyot H, Serteyn D, Kirsch K. Analytical Performance Evaluation of the New GEM(®) Premier™ 5000 in Comparison to the Epoc(®) Blood Gas Analyzer in Horses. Vet Sci 2023 Feb 3;10(2).
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