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Theriogenology2010; 75(1); 105-114; doi: 10.1016/j.theriogenology.2010.07.015

Egg yolk plasma can replace egg yolk in stallion freezing extenders.

Abstract: Hen egg yolk is normally used as a cryoprotective agent in semen freezing extenders, but its use has sanitary and practical disadvantages. Moreover the protection afforded by egg yolk has not yet been completely elucidated. The objective of this study was to compare the egg yolk plasma fraction to whole egg yolk in stallion freezing extender. Plasma contains mainly Low Density Lipoproteins (LDL), which are widely presumed to be the cryoprotective agent in egg yolk. Plasma can be produced on an industrial scale, sterilised by gamma-irradiation and incorporated in a ready-to-use extender (our ultimate objective). Plasma samples were subjected to different doses of gamma-irradiation (3, 5, 10 kGy) without dramatic chemical changes that may affect their cryoprotective properties. Stallion semen was frozen with whole egg yolk as a control and with sterilised egg yolk plasma. A fertility trial was conducted on a total of 70 mares' cycles. Fertility per cycle was 60% after insemination of semen frozen in our control extender containing egg yolk (EY), compared to 69% for the extender containing sterilised egg yolk plasma (EYP) (P > 0.05). Post-thaw motility and membrane integrity of spermatozoa were also analysed. Motility parameters were not significantly different between extenders except for the variable VAP (for EY versus EYP, VAP: 63 μm.s(-1) versus 59 μm.s(-1), a, b: P 0.05). Membrane integrity was better preserved in EY than in EYP but the difference between extenders was small (P < 0.05). Our results demonstrated that sterilised egg yolk plasma has the potential to replace egg yolk in stallion freezing extender. This experiment led to the development of a ready-to-use extender called INRA-Freeze(®) (IMV-Technologies, France).
Copyright © 2011 Elsevier Inc. All rights reserved.
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Publication Date: 2010-09-15 PubMed ID: 20833417DOI: 10.1016/j.theriogenology.2010.07.015Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study explored the use of egg yolk plasma as a replacement for whole egg yolk in the freeze preservation of stallion semen. Researchers found that egg yolk plasma can potentially provide the same cryoprotective properties as whole egg yolk, thereby leading to the development of a new, ready-to-use preservation solution.

Objective of the Study

  • The objective of the study was to evaluate the effectiveness of egg yolk plasma in the freezing and preservation of stallion semen. Egg yolk has been traditionally used as a cryoprotective agent, yet it presents certain sanitary and practical challenges. Egg yolk also contains low density lipoproteins (LDL), which are believed to be the primary component providing cryoprotection. Thus, the researchers aimed to compare the impact of egg yolk plasma (which contains LDL) and whole egg yolk on the preservation of stallion semen.

Methodology

  • Plasma samples were subjected to different levels of gamma-irradiation (3, 5, 10 kGy) to sterilize them without affecting their cryoprotective properties.
  • Stallion semen was frozen using both traditional whole egg yolk as a control and the sterilized egg yolk plasma for comparison.
  • A fertility trial was performed involving 70 mares’ cycles to evaluate the effectiveness of the extenders (a substance used to dilute and preserve semen).

Results

  • The use of sterilized egg yolk plasma resulted in a slightly higher fertility per cycle (69%) compared to the control extender containing whole egg yolk (60%). However, this difference wasn’t significant statistically.
  • Post-thaw motility and membrane integrity of spermatozoa were also analyzed. Motility parameters were not significantly different between the two groups, except for the VAP (velocity average path) which was slightly lower in the plasma group. However, the difference was very small.
  • Membrane integrity was slightly better preserved in the whole egg yolk group than in the plasma group, but the difference was minor.

Conclusion

  • The results from the experiment indicated that sterilized egg yolk plasma has potential as a replacement for whole egg yolk in stallion semen freezing extenders, as it provides similar cryoprotection and resulted in comparable fertility rates.
  • Based on these findings, the researchers developed a new, ready-to-use extender called INRA-Freeze®, provided by IMV-Technologies, France.

Cite This Article

APA
Pillet E, Duchamp G, Batellier F, Beaumal V, Anton M, Desherces S, Schmitt E, Magistrini M. (2010). Egg yolk plasma can replace egg yolk in stallion freezing extenders. Theriogenology, 75(1), 105-114. https://doi.org/10.1016/j.theriogenology.2010.07.015

Publication

Theriogenology
ISSN: 1879-3231
NlmUniqueID: 0421510
Country: United States
Language: English
Volume: 75
Issue: 1
Pages: 105-114

Researcher Affiliations

Pillet, E
  • INRA, UMR85 Physiologie de la Reproduction et des Comportements, Nouzilly, France.
Duchamp, G
    Batellier, F
      Beaumal, V
        Anton, M
          Desherces, S
            Schmitt, E
              Magistrini, M

                MeSH Terms

                • Animals
                • Cryopreservation / methods
                • Cryopreservation / veterinary
                • Cryoprotective Agents
                • Egg Yolk
                • Female
                • Horses
                • Lipoproteins, LDL / physiology
                • Male
                • Nanoparticles / chemistry
                • Oxidative Stress
                • Particle Size
                • Pregnancy
                • Pregnancy Rate
                • Semen Analysis
                • Semen Preservation / methods
                • Semen Preservation / veterinary
                • Spermatozoa / physiology
                • Spermatozoa / ultrastructure
                • Sterilization

                Citations

                This article has been cited 14 times.
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