Elimination of repeated clot formation in mouse ascitic fluid containing arbovirus antibodies.
Abstract: Repeated clot formation in mouse ascitic fluids containing antiviral antibody was eliminated by acid precipitation of the fibrinogen.
Publication Date: 1972-08-01 PubMed ID: 4672320PubMed Central: PMC380598DOI: 10.1128/am.24.2.288-289.1972Google Scholar: Lookup
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- Journal Article
Summary
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The researchers discovered a method to prevent clot formation repeatedly in ascitic fluid taken from mice that contain antibodies fighting against arboviruses, by precipitating the fibrinogen through the use of acids.
Introduction
- The study focuses on addressing a common issue – repeated clot formation in ascitic fluid from mice.
- Ascitic fluid is a type of bodily fluid found in the peritoneal cavity, which can sometimes contain antibodies against arboviruses.
- Arboviruses are a group of viruses that are transmitted by insects like mosquitoes and ticks.
Methods
- Repeated clot formation, a recurring problem during laboratory studies, hampers the ability to accurately study the presence and function of arbovirus antibodies in mouse ascitic fluids.
- To eliminate this clotting, the researchers used acid to precipitate out the fibrinogen from the ascitic fluid. Fibrinogen is a protein responsible for blood clotting in the body. Upon its removal, the propensity for the fluid to form clots is significantly reduced.
Results and Conclusion
- Through successful acid precipitation of fibrinogen, the researchers were able to prevent the formation of repetitive clots in the mouse ascitic fluid, thus facilitating smoother laboratory studies on arbovirus antibodies.
- Their findings underpin an effective method for researchers who wish to study arbovirus antibodies in mice without the issue of repeated clotting, increasing the efficiency and accuracy of lab tests and experimental studies in this area.
Cite This Article
APA
Chiewsilp D, McCown JM.
(1972).
Elimination of repeated clot formation in mouse ascitic fluid containing arbovirus antibodies.
Appl Microbiol, 24(2), 288-289.
https://doi.org/10.1128/am.24.2.288-289.1972 Publication
Researcher Affiliations
MeSH Terms
- Acetates
- Animals
- Antibodies / analysis
- Arboviruses / immunology
- Ascitic Fluid / immunology
- Chemical Precipitation
- Complement Fixation Tests
- Dengue Virus / immunology
- Encephalitis Virus, Japanese / immunology
- Encephalitis Virus, St. Louis / immunology
- Encephalitis Virus, Western Equine / immunology
- Encephalitis Viruses / immunology
- Encephalitis Viruses, Tick-Borne / immunology
- Fibrinogen
- Hemagglutination Inhibition Tests
- Hydrogen-Ion Concentration
- Immunization
- Mice / immunology
- Neutralization Tests
- Sindbis Virus / immunology
- Yellow fever virus / immunology
References
This article includes 4 references
- Russell PK, Chiewsilp D, Brandt WE. Immunoprecipitation analysis of soluble complement-fixing antigens of dengue viruses.. J Immunol 1970 Oct;105(4):838-45.
- KENT JF, FIFE EH Jr. Precise standardization of reagents for complement fixation.. Am J Trop Med Hyg 1963 Jan;12:103-16.
- KISTLER P. [Fractionation of human blood plasma proteins].. Blut 1960;6(Suppl):414-23.
- CLARKE DH, CASALS J. Techniques for hemagglutination and hemagglutination-inhibition with arthropod-borne viruses.. Am J Trop Med Hyg 1958 Sep;7(5):561-73.
Citations
This article has been cited 5 times.- Woodman DR, McManus AT, Eddy GA. Extension of the mean time to death of mice with a lethal infection of Venezuelan equine encephalomyelitis virus by antithymocyte serum treatment. Infect Immun 1975 Nov;12(5):1006-11.
- Catanzaro PJ, Brandt WE, Russell PK. The influence of arboviral infection on the susceptibility of cultured cells to immune injury in vitro. Am J Pathol 1975 Jul;80(1):91-100.
- Brandt WE, Russell PK. Influence of cell type and virus upon virus-specific immune cytolysis. Infect Immun 1975 Feb;11(2):330-3.
- Boonpucknavig S, Bhamarapravati N, Nimmannitya S, Phalavadhtana A, Siripont J. Immunofluorescent staining of the surfaces of lymphocytes in suspension from patients with dengue hemorrhagic fever. Am J Pathol 1976 Oct;85(1):37-48.
- Eckels KH, Hetrick FM, Russell PK. Virion and soluble antigens of japanese encephalitis virus. Infect Immun 1975 May;11(5):1053-60.
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