Endocrine and ovarian responses to combined estradiol benzoate-sulpiride in seasonally anovulatory mares treated with kisspeptin.
Abstract: The objective of this experiment was to determine if incorporation of kisspeptin-10 (Kp10) into treatment with estradiol benzoate (EB) and sulpiride to induce early cyclicity would result in greater endocrine responses and a greater number of mares responding with either follicle(s) > 30 mm or ovulation within 25 days of treatment. Eighteen anestrous mares were blocked by breed, body condition, and age before random assignment to treatment or control. All mares received 50 mg EB before receiving osmotic minipumps containing either saline (n = 9) or Kp10 (50 μg/hour; n = 9) one day later. The next day, all mares received 3 g sulpiride. Serial blood sampling occurred after pump placement and continued daily for 25 days. Transrectal ultrasounds were performed regularly to monitor ovarian activity. Data were analyzed by Student's t-test or ANOVA with repeated measures. Seven Kp10-treated mares responded compared to only 4 saline-treated mares. Mean days from sulpiride treatment to ovarian response was less in Kp10-treated mares (13.7 ± 1.1 d, P ≤ 0.01) compared to saline-treated mares (35.9 ± 7.8 d). Plasma prolactin increased (P < 0.001) in response to sulpiride in all mares; however, prolactin was higher (P < 0.05) in Kp10-treated mares. Plasma LH increased in all mares beginning 5 days after sulpiride but was greater (P < 0.0001) in Kp10-treated mares. Plasma FSH concentrations did not differ between groups. In conclusion, incorporation of Kp10 potentiated the prolactin and LH responses to EB-sulpiride and resulted in more mares responding with early ovarian activity.
Copyright © 2022 Elsevier B.V. All rights reserved.
Publication Date: 2022-09-30 PubMed ID: 36201980DOI: 10.1016/j.anireprosci.2022.107087Google Scholar: Lookup
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- Randomized Controlled Trial
- Veterinary
- Journal Article
Summary
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The study aimed to determine the effectiveness of integrating kisspeptin-10 (Kp10) into an estradiol benzoate (EB) and sulpiride treatment to induce early cyclicity in anovulatory mares. The research found that Kp10 treatment was successful in stimulating greater endocrine responses and encouraging a larger number of mares to respond with follicle growth or ovulation within 25 days.
Methodology
- The experiment included eighteen anestrous mares that were divided into groups based on breed, body condition, and age and then randomly assigned for treatments or control.
- All mares received 50 mg EB, and then on the next day, osmotic minipumps containing either Kp10 (50 μg/hour) or saline were supplied to them.
- Another day later, all mares received 3 g sulpiride whereupon blood samples were taken daily for 25 days and transrectal ultrasounds were performed on the mares to observe their ovarian activities.
Findings
- Seven out of the nine mares treated with Kp10 responded to the treatment and showed signs of ovarian activity, while only four out of the nine saline-treated mares responded.
- It was noted that the mares treated with Kp10 showed ovarian response sooner (in around 13.7 days) than the mares treated with saline (around 35.9 days).
- There was an increase in plasma prolactin in all mares due to the sulpiride treatment, however, this increase was more pronounced in the Kp10-treated mares.
- Plasma LH increased in all mares after 5 days of sulpiride treatment, but the increase was significantly higher in Kp10-treated mares. Plasma FSH concentrations showed no notable difference between the groups.
Conclusion
The research concluded that the use of Kp10 could enhance the prolactin and LH responses to the EB-sulpiride treatment, leading to a greater number of mares showing early ovarian activity. Thus, the incorporation of Kp10 into treatment could be beneficial for inducing early cyclicity in anovulatory mares.
Cite This Article
APA
Bailey VN, Sones JL, Camp CM, Gomes VCL, Oberhaus EL.
(2022).
Endocrine and ovarian responses to combined estradiol benzoate-sulpiride in seasonally anovulatory mares treated with kisspeptin.
Anim Reprod Sci, 247, 107087.
https://doi.org/10.1016/j.anireprosci.2022.107087 Publication
Researcher Affiliations
- School of Animal Sciences, Louisiana Agricultural Experiment Station, Louisiana State University Agricultural Center, Baton Rouge, LA, USA.
- Department of Veterinary Clinical Sciences, Louisiana State University School of Veterinary Medicine, Baton Rouge, LA, USA.
- School of Animal Sciences, Louisiana Agricultural Experiment Station, Louisiana State University Agricultural Center, Baton Rouge, LA, USA.
- Department of Veterinary Clinical Sciences, Louisiana State University School of Veterinary Medicine, Baton Rouge, LA, USA.
- School of Animal Sciences, Louisiana Agricultural Experiment Station, Louisiana State University Agricultural Center, Baton Rouge, LA, USA. Electronic address: eoberhaus@agcenter.lsu.edu.
MeSH Terms
- Horses
- Female
- Animals
- Sulpiride / pharmacology
- Luteinizing Hormone
- Prolactin
- Follicle Stimulating Hormone
- Kisspeptins / pharmacology
- Anovulation / veterinary
- Estradiol / pharmacology
- Ovulation
- Horse Diseases
Conflict of Interest Statement
Conflict of interest The authors declare no conflict of interest.
Citations
This article has been cited 1 times.- Khan IU, Khairullah AR, Khan AY, Rehman AU, Mustofa I. Strategic approaches to improve equine breeding and stud farm outcomes. Vet World 2025 Feb;18(2):311-328.
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