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Home / Equine Research Bank / Reprod Domest Anim / Endometrial angiogenesis induced by uterine insufflation wit...
Reproduction in domestic animals = Zuchthygiene2021; 56(9); 1176-1183; doi: 10.1111/rda.13958

Endometrial angiogenesis induced by uterine insufflation with an oxygen-ozone gas mixture in mares.

Abstract: Ozone (O ) therapy has been used to improve peripheral tissue oxygenation in humans and domestic animals. The goal of the present study was to characterize histological changes in the endometria of healthy equines following tissue exposure to gas mixtures enriched with different concentrations of O . Cycling mares without endometrial degeneration were divided into three groups according to treatment (n = 9 mares/group). The uteri from the O , ½O and control groups were insufflated for 3 min with gas containing 42, 21 and 0 μg O ml , respectively. Treatments were performed every three days from D0 to D6. Endometrial samples were collected immediately before the first treatment and 24 hr after the last treatment. The following nine histological parameters were evaluated: (i) the number of endometrial blood vessels, (ii) endometrial vascular degree (EVD), (iii) increase rate of blood vessels, (iv) increase rate of EVD, (v) glandular total area, (vi) glandular lumen area, (vii) intraglandular secretion area, (viii) glandular epithelial height and (ix) luminal epithelial height. In the O group, a positive effect from treatment (p < .01) was detected for all vascular parameters (i, ii, iii and iv), glandular total area, intraglandular secretion area and glandular epithelial height. Compared to the control group, the ½O group had greater (p < .01) EVD (84.1 ± 12%) and a higher increase rate of blood vessels (151.9 ± 47.1%). Uterine insufflation with low or intermediate concentrations of the O -O gas mixture induced endometrial angiogenesis. Morphometry, but not morphology, of the endometrial glands was affected by local O therapy. These findings would be of great significance for the development of new therapies for infertility in mares.
© 2021 Wiley-VCH GmbH.
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Publication Date: 2021-06-03 PubMed ID: 34021934DOI: 10.1111/rda.13958Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research aimed to understand how ozone therapy affects histological changes in a horse’s endometrium. A successful outcome involves promoting endometrial angiogenesis and changes to endometrial glands, which can contribute to developing new infertility treatments in mares.

Methodology

  • The trial involved healthy cycle mares that didn’t display signs of endometrial degeneration, divided into three groups: a control group, a half-ozone group, and a full-ozone group.
  • The groups underwent uterine insufflation with different gas mixtures: a control group received 0μg O3/ml; the half-ozone group got 21μg O3/ml, and the full ozone group received 42μg O3/ml.
  • The procedure was carried out every three days, starting from the procedure’s first day (D0) until the seventh day (D6).
  • Endometrial samples were collected before the first treatment and after 24 hours from the last treatment for testing.

Parameters Evaluated

  • Several histological parameters were assessed, including the number and increase rate of endometrial blood vessels, endometrial vascular degree (EVD), and increase rate of EVD.
  • The glandular aspects studied were the total area, lumen area, intraglandular secretion area, glandular epithelial height, and luminal epithelial height.

Results

  • In the full-ozone group, there was a significant positive effect on vascular parameters, glandular total area, intraglandular secretion area, and glandular epithelial height.
  • Compared to the control group, the half-ozone group displayed higher EVD (84.1 ± 12%) and a greater increase rate of blood vessels (151.9 ± 47.1%).

Conclusion

  • Uterine insufflation with low or intermediate concentrations of the oxygen-ozone gas mixture stimulated endometrial angiogenesis.
  • The study revealed that the morphometry of the endometrial glands was affected by local ozone therapy, but there were no changes in their morphology.
  • The results of this research could have significant implications for formulating new medical therapies to combat infertility in mares.

Cite This Article

APA
Camargo Ferreira J, Silva Amaral R, Mara Cardoso L, Panelli Marchió S, Aldrovani Rodrigues M, Fonseca Alves CE, de Meira C. (2021). Endometrial angiogenesis induced by uterine insufflation with an oxygen-ozone gas mixture in mares. Reprod Domest Anim, 56(9), 1176-1183. https://doi.org/10.1111/rda.13958

Publication

Reproduction in domestic animals = Zuchthygiene
ISSN: 1439-0531
NlmUniqueID: 9015668
Country: Germany
Language: English
Volume: 56
Issue: 9
Pages: 1176-1183

Researcher Affiliations

Camargo Ferreira, Jair
  • Post-graduation Program in Animal Science, University of Franca, Franca, Brazil.
Silva Amaral, Rafael
  • Post-graduation Program in Animal Science, University of Franca, Franca, Brazil.
Mara Cardoso, Letícia
  • Post-graduation Program in Animal Science, University of Franca, Franca, Brazil.
Panelli Marchió, Sophia
  • Post-graduation Program in Animal Science, University of Franca, Franca, Brazil.
Aldrovani Rodrigues, Marcela
  • Post-graduation Program in Animal Science, University of Franca, Franca, Brazil.
Fonseca Alves, Carlos Eduardo
  • Department of Veterinary Clinics, School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Botucatu, Brazil.
de Meira, Cezinande
  • Department of Animal Reproduction and Veterinary Radiology, School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Botucatu, Brazil.

MeSH Terms

  • Animals
  • Endometrium / blood supply
  • Endometrium / drug effects
  • Female
  • Horses
  • Insufflation / veterinary
  • Neovascularization, Physiologic / drug effects
  • Oxygen / pharmacology
  • Ozone / pharmacology
  • Uterus / surgery

Grant Funding

  • 2019/22728-2 / FAPESP
  • 2019/11680-9 / FAPESP
  • 2019/11450-3 / FAPESP
  • 001 / CAPES

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Citations

This article has been cited 1 times.
  1. Köhne M, Hofbauer L, Böttcher D, Tönissen A, Hegger A, Görgens A, Ulrich R, Sieme H. Comparison of systemic trimethoprim-sulfadimethoxine treatment and intrauterine ozone application as possible therapies for bacterial endometritis in equine practice.. Front Vet Sci 2023;10:1102149.
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