Endometrial connexin expression in the mare and pig: evidence for the suppression of cell-cell communication in uterine luminal epithelium.
Abstract: This investigation examines the relationship between implantation strategy and gap junction protein expression in uterine endometrium. The pattern of gap junction and connexin protein expression was analyzed in porcine and equine endometrium from cycling and pregnant animals using electron microscopy and immunocytochemistry. Functional analysis of cell-cell communication was also monitored by laser cytometry in primary cultures of endometrial epithelial cells. Gap junctions were detected in endometrial stroma of cycling and pregnant animals, which was correlated with immunoreactive Cx43 within stromal fibroblasts and vascular elements. No Cx26, Cx32, or Cx43 immunostaining was detected in luminal endometrial epithelium in either the mare or the pig at any stage of the estrous cycle or pregnancy. In contrast, endometrial glands of the mare exhibited a spatiotemporal pattern of Cx43 expression in the apicolateral plasma membrane which, when present, colocalized with the tight junction-associated protein, ZO-1. Uterine glandular Cx43 expression in mares was present from day 3 postovulation through day 14 of diestrus and until day 23 of pregnancy, whereas Cx43 was absent within uterine glands during seasonal anestrus, estrus, and after day 30 of pregnancy. Primary cultures of equine endometrial epithelial cells expressed both immunoreactive Cx43 and significant gap junction-mediated intercellular communication (GJIC) which was rapidly upregulated by 1.0 mM 8-bromo-cAMP or blocked with 1.0 mM octanol. No GJIC or connexin protein was detected in cultured porcine epithelial cells despite incubation with a variety of agents, including 8-bromo-cAMP, steroid hormones, retinoic acid, and/or prolactin. Junctional communication in endometrial epithelium of domestic farm animals is different than that reported for species exhibiting invasive implantation. The absence of GJIC in uterine luminal epithelium of the gilt and mare may be involved in limiting trophoblast invasiveness.
Publication Date: 1998-07-21 PubMed ID: 9669753DOI: 10.1002/(SICI)1097-0185(199807)251:3<277::AID-AR1>3.0.CO;2-TGoogle Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
- Research Support
- U.S. Gov't
- Non-P.H.S.
Summary
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The research paper investigates the role of cellular communication in the endometrium (lining of the uterus) of mares and pigs, in relation to embryo implantation. It identified a difference in certain proteins’ expression related to cell connectivity in these animals, which could possibly inhibit the embryo from embedment into the uterus.
Investigation of Connexin Expression
- This study investigates the correlation between embryo implantation strategy and the expression of gap junction proteins (connexins) in the uterine endometrium.
- Connexins are crucial for cell-to-cell communication in mammalian tissues, and this study aims to identify the difference in the expression of these proteins in pigs and mares during their reproductive cycles or pregnancy.
- Connexin 43 (Cx43) was found in the endometrial stroma (the supportive structure around the uterine lining) in both pregnant and cycling animals, linked to Cx43 in stromal fibroblasts and vascular components.
Endometrial Glands and Connexins
- The existence of connexin proteins was not detected in the luminal endometrial epithelium (cells directly lining the uterus). This was the same for both pigs and mares, regardless of the stage of the reproductive cycle or pregnancy.
- However, endometrial glands in mares showed a temporal and spatial pattern of Cx43 expression. When present, this expression coincided with ZO-1, a protein associated with tight junctions in cells.
- Expression of Cx43 in uterine glands of mares was observed from the 3rd to the 14th day of the diestrus period and until the 23rd day of pregnancy. The absence of Cx43 within uterine glands happened during estrus, seasonal anestrus, and after the 30th day of pregnancy.
Cultured Cells and Connexins
- Primary cultures of equine endometrial epithelial cells showed significant expressions of Cx43 and gap junction-mediated intercellular communication (GJIC). The study also revealed that GJIC could be quickly increased by specific agents but could also be blocked.
- On the other hand, cultured porcine epithelial cells did not show any GJIC or connexin proteins, even when incubated with various substances.
Implication of Research Findings
- These findings point out that the junctional communication in endometrial epithelium of farm animals is different from the cellular communication observed in species where implantation is invasive.
- The absence of GJIC in the uterine epithelium of pigs and mares might restrict the invasiveness of the trophoblast, the layer of cells that develop into the placenta. This could potentially be a limiting factor for embryo implantation.
Cite This Article
APA
Day WE, Bowen JA, Barhoumi R, Bazer FW, Burghardt RC.
(1998).
Endometrial connexin expression in the mare and pig: evidence for the suppression of cell-cell communication in uterine luminal epithelium.
Anat Rec, 251(3), 277-285.
https://doi.org/10.1002/(SICI)1097-0185(199807)251:3<277::AID-AR1>3.0.CO;2-T Publication
Researcher Affiliations
- Department of Veterinary Anatomy and Public Health, Texas A&M University, College Station 77843-4458, USA.
MeSH Terms
- Animals
- Cell Communication
- Cells, Cultured
- Connexins / metabolism
- Endometrium / cytology
- Endometrium / metabolism
- Endometrium / ultrastructure
- Epithelial Cells / cytology
- Epithelial Cells / metabolism
- Epithelial Cells / ultrastructure
- Estrus
- Female
- Fluorescent Antibody Technique, Indirect
- Gap Junctions / metabolism
- Gap Junctions / ultrastructure
- Horses / metabolism
- Membrane Proteins / metabolism
- Phosphoproteins / metabolism
- Pregnancy
- Swine / metabolism
- Zonula Occludens-1 Protein
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