Endometrial nitric oxide synthase activity in mares susceptible or resistant to persistent breeding-induced endometritis and the effect of a specific iNOS inhibitor in vitro.
Abstract: Emerging research suggests that the nitric oxide system may play a role in persistent breeding-induced endometritis (PBIE) in the mare. Differences in uterine nitric oxide (NO) levels between mares susceptible or resistant to PBIE and a dose-dependent inhibitory effect of NO on uterine contractility have been demonstrated. The objectives of this study were to investigate the difference in total nitric oxide synthase (NOS) activity of the endometrium between susceptible and resistant mares and the effect of a specific inducible nitric oxide synthase (iNOS) inhibitor on the endometrial NOS activity in vitro. Six susceptible and six resistant mares were selected based on preset criteria and the results of an intrauterine challenge with killed spermatozoa during oestrus. Endometrial biopsy samples were collected 24 hr post-challenge and cultured at 37°C for 24 hr in L-arginine supplemented minimum essential medium with or without a specific iNOS inhibitor (1,400 W dihydrochloride, 1 mM). The medium and the cultured endometrial tissue were collected after 24 hr of culture and assayed for NO and total protein, respectively. Total NO content of the medium, normalized to endometrial tissue wet weight or total protein, was used as a measure of endometrial NOS activity. Non-parametric tests were applied for statistical analysis. Susceptible mares had significantly greater endometrial NOS activity than resistant mares. The iNOS inhibitor treatment significantly reduced NOS activity in endometrial samples derived from susceptible and resistant mares. These findings provide a basis for in vivo testing of specific iNOS inhibitors as preventative or therapeutic options for PBIE in mares.
© 2018 Blackwell Verlag GmbH.
Publication Date: 2018-03-14 PubMed ID: 29537110DOI: 10.1111/rda.13162Google Scholar: Lookup
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- Journal Article
Summary
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The research article is a study on the role nitric oxide system plays in persistent breeding-induced endometritis in mares. Scientists found a difference in nitric oxide synthase activity between mares susceptible or resistant to this condition, and demonstrated the inhibitory effect of a specific nitric oxide synthase inhibitor on endometrial activity in vitro.
Research Objective
- The primary objective of this research study was to explore the difference in the total activity of nitric oxide synthase (NOS) in the endometrium between mares that are resistant or susceptible to persistent breeding-induced endometritis (PBIE). It also aimed to determine the impact of a particular inducible nitric oxide synthase (iNOS) inhibitor on endometrial NOS activity in vitro.
Research Methodology
- The researchers selected six mares that were resistant to PBIE and six which were susceptible based on well-defined criteria, and the result of an intrauterine challenge with killed spermatozoa during oestrus.
- After the challenge, endometrial biopsy samples were procured from the mares and cultured in medium supplemented with L-arginine with or without an iNOS inhibitor.
- The culture medium and the endometrial tissues were collected and analyzed for NO and total protein respectively, 24 hours after culture.
- The endometrial NOS activity was determined by normalizing the total NO content of the medium with the wet weight or total protein of the endometrial tissue.
- Non-parametric tests were deployed for statistical analysis.
Key Findings of the Research
- Mares that were susceptible to PBIE showed significantly higher endometrial NOS activity than those resistant to the condition.
- The iNOS inhibitor treatment considerably lowered NOS activity in endometrial samples from both susceptible and resistant mares.
Significance of the Research
- The research concludes significant differences in endometrial NOS activity between mares susceptible and those resistant to PBIE.
- It also provides a platform for in vivo testing of specific iNOS inhibitors, which could be further explored as preventative or therapeutic options for PBIE in mares.
Cite This Article
APA
Khan FA, Chenier TS, Foster RA, Hewson J, Scholtz EL.
(2018).
Endometrial nitric oxide synthase activity in mares susceptible or resistant to persistent breeding-induced endometritis and the effect of a specific iNOS inhibitor in vitro.
Reprod Domest Anim, 53(3), 718-724.
https://doi.org/10.1111/rda.13162 Publication
Researcher Affiliations
- Department of Population Medicine, Ontario Veterinary College, University of Guelph, Guelph, ON, Canada.
- Department of Population Medicine, Ontario Veterinary College, University of Guelph, Guelph, ON, Canada.
- Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, ON, Canada.
- Department of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, ON, Canada.
- Department of Population Medicine, Ontario Veterinary College, University of Guelph, Guelph, ON, Canada.
MeSH Terms
- Animals
- Disease Susceptibility / veterinary
- Endometritis / enzymology
- Endometritis / veterinary
- Endometrium / enzymology
- Enzyme Inhibitors / pharmacology
- Female
- Horse Diseases / enzymology
- Horses
- Male
- Nitric Oxide / biosynthesis
- Nitric Oxide Synthase / metabolism
- Nitric Oxide Synthase Type II / antagonists & inhibitors
- Spermatozoa
Citations
This article has been cited 3 times.- Wojtysiak K, Ryszka W, Stefaniak T, Król J, Kozdrowski R. Changes in the Secretion of Anti-Inflammatory Cytokines and Acute-Phase Proteins in the Uterus after Artificial Insemination in the Mare.. Animals (Basel) 2020 Dec 19;10(12).
- Rytel L, Gonkowski S. The Influence of Bisphenol a on the Nitrergic Nervous Structures in the Domestic Porcine Uterus.. Int J Mol Sci 2020 Jun 26;21(12).
- Canisso IF, Segabinazzi LGTM, Fedorka CE. Persistent Breeding-Induced Endometritis in Mares - a Multifaceted Challenge: From Clinical Aspects to Immunopathogenesis and Pathobiology.. Int J Mol Sci 2020 Feb 20;21(4).
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