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Home / Equine Research Bank / J Vet Intern Med / Endoscopic Evaluation of Angiogenesis in the Large Airways o...
Journal of veterinary internal medicine2016; 30(2); 671-674; doi: 10.1111/jvim.13890

Endoscopic Evaluation of Angiogenesis in the Large Airways of Horses with Heaves Using Narrow Band Imaging.

Abstract: Heaves is a severe debilitating condition of horses, characterized by lower airway inflammation and permanent structural changes of the bronchial wall. Chronic inflammation promotes the formation of new vessels, a phenomenon known as angiogenesis. Narrow band imaging (NBI) endoscopy is a noninvasive technique that enhances the visualization of submucosal vessels, and commonly is employed for the study of angiogenesis in human patients. Objective: Using NBI, we aimed to determine whether or not the central airways of horses with heaves undergo angiogenesis. Methods: Horses with heaves during exacerbation of the disease (n = 5) and healthy controls (n = 6). Methods: A library of NBI images was established from previously recorded videoendoscopies. Images were acquired by an operator blinded to horse ID. Images were obtained from 3 sites: 130 from the trachea (14 ± 9.3 [mean ± SD] images per horse with heaves and 10 ± 5.4 from controls; P = .45), 58 from the carina (5.4 ± 3.2 from horses with heaves and 5.2 ± 2.8 fromn controls; P > .99) and 167 from the intermediate bronchi (17.8 ± 6.7 from horses with heaves and 13 ± 5.6 from controls; P = .17). Using dedicated stereology software (NewCAST, Visiopharm; Denmark), the volume density of superficial and deep vessels was calculated blindly by point counting at each site for all horses. Results: In the trachea, the volume density of superficial vessels was increased in horses with heaves compared to controls (P = .02). No difference was found between groups for the volume density of both superficial and deep vessels at the carina or intermediate bronchi. Conclusions: NBI imaging of the airways was easily performed in standing sedated horses.
Copyright © 2016 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals, Inc. on behalf of the American College of Veterinary Internal Medicine.
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Publication Date: 2016-03-01 PubMed ID: 26926661PubMed Central: PMC4913588DOI: 10.1111/jvim.13890Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research study investigates if horses with a severe respiratory condition called “heaves” undergo angiogenesis (the formation of new blood vessels from existing ones) in their central airways. This was analyzed using a non-invasive technique, narrow band imaging (NBI), which improves visualization of submucosal vessels. The results suggested increased superficial vessels in the trachea of horses with heaves.

Objective and Methods

  • The main objective of this research was to detect if horses suffering from heaves develop angiogenesis in their central airways. Angiogenesis is a natural biological process that leads the formation of new blood vessels.
  • The researchers used a technique known as narrow band imaging (NBI), a non-invasive endoscopy that enhances the visibility of submucosal vessels.
  • The study was conducted by examining both healthy horses and those with heaves during exacerbation. Images were collected from different sites including the trachea, the carina (the point where the trachea divides), and the intermediate bronchi.
  • These images were later analyzed using dedicated stereology software (NewCAST), which helped calculate the volume density of both superficial and deep vessels of the central airways.

Results

  • The study found an increased volume density of superficial vessels in the trachea of horses with heaves compared to the control group. This indicates the presence of angiogenesis in the affected horses.
  • However, no significant difference was found in the volume density of both superficial and deep vessels at the carina or in the intermediate bronchi between the two groups.

Conclusions

  • The results suggest that NBI imaging is an effective approach for evaluating angiogenesis in standing sedated horses’ airways.
  • The study provides a useful insight into the pathological changes that occur in horses suffering from heaves, which may help in the development of therapeutic interventions.
  • Despite angiogenesis being noted in the trachea of horses with heaves, no significant changes were observed in other parts of the airways. More studies might be needed to further understand the systemic effects of heaves on the equine respiratory system.

Cite This Article

APA
Herteman N, Bullone M, Lavoie JP. (2016). Endoscopic Evaluation of Angiogenesis in the Large Airways of Horses with Heaves Using Narrow Band Imaging. J Vet Intern Med, 30(2), 671-674. https://doi.org/10.1111/jvim.13890

Publication

Journal of veterinary internal medicine
ISSN: 1939-1676
NlmUniqueID: 8708660
Country: United States
Language: English
Volume: 30
Issue: 2
Pages: 671-674

Researcher Affiliations

Herteman, N
  • Département de Sciences Cliniques, Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, QC, Canada.
Bullone, M
  • Département de Sciences Cliniques, Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, QC, Canada.
Lavoie, J-P
  • Département de Sciences Cliniques, Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, QC, Canada.

MeSH Terms

  • Airway Remodeling
  • Animals
  • Bronchi / blood supply
  • Bronchi / diagnostic imaging
  • Case-Control Studies
  • Horse Diseases / diagnostic imaging
  • Horse Diseases / pathology
  • Horses
  • Lung Diseases, Obstructive / diagnostic imaging
  • Lung Diseases, Obstructive / pathology
  • Lung Diseases, Obstructive / veterinary
  • Neovascularization, Pathologic / diagnostic imaging
  • Neovascularization, Pathologic / veterinary

Grant Funding

  • MOF102751 / Canadian Institutes of Health Research

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This article includes 9 references
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Citations

This article has been cited 2 times.
  1. Millares-Ramirez EM, Lavoie JP. Bronchial angiogenesis in horses with severe asthma and its response to corticosteroids.. J Vet Intern Med 2021 Jul;35(4):2026-2034.
    doi: 10.1111/jvim.16159pubmed: 34048095google scholar: lookup
  2. Winter RL, Tian Y, Caldwell FJ, Seeto WJ, Koehler JW, Pascoe DA, Fan S, Gaillard P, Lipke EA, Wooldridge AA. Cell engraftment, vascularization, and inflammation after treatment of equine distal limb wounds with endothelial colony forming cells encapsulated within hydrogel microspheres.. BMC Vet Res 2020 Feb 4;16(1):43.
    doi: 10.1186/s12917-020-2269-ypubmed: 32019556google scholar: lookup
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