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Veterinary research communications2023; 47(3); 1471-1478; doi: 10.1007/s11259-023-10101-5

Enterococcus moraviensis EMo 1-1Nik of horse origin:characteristics and potential bacteriocin-producing strain.

Abstract: Nowadays, developed more precisious identification techniques have allowed to validate newer enterococcal species. Among them, the species Enterococcus moraviensis was also validated, at first from surface waters. However, in this study, characteristics and potential to bacteriocin production by the strain E. moraviensis EMo 1-1Nik isolated from buccal mucosa of Slovak warm-blood horse breed has been studied. BLASTn analysis allotted this strain to the species E. moraviensis with percentage identity BLASTn 16S rRNA sequence in the strain up to 100% (99.93% similarity with E. moraviensis NR113937.1). The strain EMo 1-1Nik has been provided with GenBank accession number MW326085. It is hemolysis-negative (γ-hemolysis), deoxyribonuclease-negative and gelatinase-negative; absent of virulence factor genes, low-grade biofilm-positive (0.133 ± 0.36), mostly susceptible to tested antibiotics. Moreover, 60% of EMo1-1Nik colonies were found as bacteriocin-producing against the principal indicator Enterococcus avium EA5. The concentrated substance (CS, pH 4.5) of EMo1-1Nik showed the inhibitory activity against EA5 strain (800 AU/mL); CSs with pH 6.3 and 7.3 reached inhibitory activity 100 AU/mL against EA5 strain. CS was thermo-stable and it does not lost activity after enzymes treatment. Oppositelly, EMo 1-1Nik was susceptible to Mundticin EM 41/3 (800 AU/mL) produced by horse fecal strain E. mundtii EM 41/3 and enterocins (up to 51 200 AU/mL). In spite of the preliminary results, it has been shown a potential to produce bacteriocin substance of the safe strain E. moraviensis EMo1-1Nik. The additional studies are in processing.
© 2023. The Author(s), under exclusive licence to Springer Nature B.V.
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Publication Date: 2023-03-16 PubMed ID: 36929156PubMed Central: 4707231DOI: 10.1007/s11259-023-10101-5Google Scholar: Lookup
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Summary

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This research investigates the strain Enterococcus moraviensis EMo 1-1Nik, a bacterium isolated from a horse breed’s buccal mucosa, exploring its potential as a bacteriocin-producing strain.

Background

  • This study examines E. moraviensis EMo 1-1Nik, a bacterial strain isolated from a Slovak warm-blood horse breed. It is part of the Enterococcus species, a group of bacteria that has been recently identified with more precisious techniques.
  • The focus is on the potential of E. moraviensis EMo 1-1Nik as a bacteriocin-producing strain. Bacteriocins are proteins produced by bacteria that inhibit the growth of similar or closely related bacterial strain.

Research Methodology

  • The researchers used BLASTn analysis to categorize the strain as E. moraviensis. The sequence in the strain matched up to 100% with other sequences (99.93% similar to E. moraviensis NR113937.1), validating its classification.
  • The strain was given the GenBank accession number MW326085 to officially record its genomic sequence.

Findings

  • The researchers discovered that E. moraviensis EMo 1-1Nik is hemolysis-negative, deoxyribonuclease-negative and gelatinase-negative, indicating that it lacks certain enzymes.
  • The strain also lacks virulence factor genes and has a low capacity for biofilm formation.
  • Furthermore, the strain proved to be mostly susceptible to the tested antibiotics.
  • Interestingly, 60% of EMo1-1Nik colonies were found to produce bacteriocins against Enterococcus avium EA5, a principal indicator strain. This suggests its potential for broader antimicrobial applications.

Inhibitory Activity of Concentration Substances (CS)

  • Concentration substances that have pH of 4.5 from EMo1-1Nik showed inhibitory activity against EA5 strain.
  • Concentration substances with pH 6.3 and 7.3 also showed inhibitory activity, albeit much less than the strain with pH 4.5.
  • The concentration substance was found to be thermo-stable and maintained its activity even after enzyme treatment.

Susceptibility to Other Bacteriocins

  • The strain EMo 1-1Nik was found to be susceptible to Mundticin EM 41/3, a bacteriocin produced by the E. mundtii EM 41/3 horse fecal strain, and to enterocins.

Conclusion

  • This study concludes that the E. moraviensis EMo1-1Nik strain of a Slovak warm-blood horse breed shows potential as a producer of bacteriocin, a substance that inhibits the growth of similar bacteria.
  • More research is currently underway to substantiate these preliminary findings.

Cite This Article

APA
Lauková A, Styková E, Focková V, Maďar M. (2023). Enterococcus moraviensis EMo 1-1Nik of horse origin:characteristics and potential bacteriocin-producing strain. Vet Res Commun, 47(3), 1471-1478. https://doi.org/10.1007/s11259-023-10101-5

Publication

Veterinary research communications
ISSN: 1573-7446
NlmUniqueID: 8100520
Country: Switzerland
Language: English
Volume: 47
Issue: 3
Pages: 1471-1478

Researcher Affiliations

Lauková, Andrea
  • Centre of Biosciences of the Slovak Academy of Sciences, Institute of Animal Physiology, Šoltésovej 4-6, 040 01, Košice, Slovakia. laukova@saske.sk.
Styková, Eva
  • University of Veterinary Medicine and Pharmacy in Košice, Clinic of Horses and Department of Microbiology and Imunology, Komenského 73, 041 81, Košice, Slovakia.
Focková, Valentína
  • Centre of Biosciences of the Slovak Academy of Sciences, Institute of Animal Physiology, Šoltésovej 4-6, 040 01, Košice, Slovakia.
Maďar, Marián
  • University of Veterinary Medicine and Pharmacy in Košice, Clinic of Horses and Department of Microbiology and Imunology, Komenského 73, 041 81, Košice, Slovakia.

MeSH Terms

  • Horses
  • Animals
  • Hemolysis
  • RNA, Ribosomal, 16S / genetics
  • Bacteriocins / genetics
  • Bacteriocins / pharmacology
  • Anti-Bacterial Agents / pharmacology
  • Horse Diseases

Grant Funding

  • APP0253 (2022)/APVV-16-0203 / Slovak Academy of Sciences Agency/Slovak Research and Development Agency

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