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Home / Equine Research Bank / Appl Environ Microbiol / Enumeration of anaerobic bacterial microflora of the equine ...
Applied and environmental microbiology1988; 54(9); 2155-2160; doi: 10.1128/aem.54.9.2155-2160.1988

Enumeration of anaerobic bacterial microflora of the equine gastrointestinal tract.

Abstract: Samples from the duodenum, jejunum, and ileum, as well as from the cecum and colon, were obtained from 11 mature grass-fed horses. Viable counts of total culturable and proteolytic bacteria were made on habitat-simulating media containing 40% clarified ruminal fluid. The mean pHs in the duodenum, jejunum, and ileum were 6.32, 7.10, and 7.47, respectively; the mean pH decreased to 6.7 in the hindgut. The acetate concentration increased along the length of the small intestine and was the only volatile fatty acid present in this gut segment. Molar proportions of acetate, propionate, and butyrate in the hindgut were 85:10:3. Differences in bacterial counts on habitat-simulating media containing equine cecal fluid or clarified ruminal fluid were negligible. Bacterial counts showed a substantial population in the duodenum (ca. 2.9 x 10(6) per g [wet weight] of sample), and this increased to 29.0 x 10(6) in the jejunum and 38.4 x 10(6) in the ileum. Proteolytic bacteria formed a high proportion of the total culturable bacteria, especially in duodenal samples. Counts of proteolytic bacteria per gram (wet weight) of sample were 3.0 x 10(6), 15.6 x 10(6), and 22.0 x 10(6) in the duodenum, jejunum, and ileum, respectively. There was a close relationship between lumenal and mucosal bacterial counts, although actual values were lower in mucosal samples. The mucosal bacterial population in the duodenum was high relative to the lumenal population. Although the comparison of bacterial populations in the hindgut of the horse and white rhino was limited to a single animal, the results were of interest. Counts were higher in the cecum than in the colon for both the horse and the white rhino.(ABSTRACT TRUNCATED AT 250 WORDS)
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Publication Date: 1988-09-01 PubMed ID: 3190223PubMed Central: PMC202828DOI: 10.1128/aem.54.9.2155-2160.1988Google Scholar: Lookup
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  • Comparative Study
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research studied the different types and amounts of bacteria residing in various parts of the horse’s digestive system, including the small and large intestines. The study also examined the changes in acidity and concentration of three fatty acids (acetate, propionate, and butyrate) along the length of the horse’s gastrointestinal tract.

Research Method

  • The experiment was conducted on 11 mature horses fed solely on grass.
  • These horses’ digestive systems were sampled in multiple locations – duodenum, jejunum, ileum, cecum, and colon.
  • Bacterial counts were taken from these samples using a habitat-simulator media that contained 40% ruminal (derived from the rumen or the first stomach chamber in animals like cows) fluid.

Findings

  • The pH level along the small intestines increased, with each part having a different mean pH – 6.32 in the duodenum, 7.10 in the jejunum, and 7.47 in the ileum. Meanwhile, the mean pH in the hindgut (cecum and colon) was lower, at 6.7.
  • Acetate was the only volatile fatty acid found in the small intestine, and its concentration increased along the length of this organ.
  • In the hindgut, the molar proportions of acetate, propionate, and butyrate were 85:10:3.
  • The count of bacteria was significant in all parts of the small intestines, with the duodenum carrying approximately 2.9 x 10(6) per gram of sample. This number grew to 29.0 x 10(6) and 38.4 x 10(6) in the jejunum and ileum, respectively.
  • Proteolytic bacteria, which break down proteins, made up a large proportion of the overall bacterial count, particularly in the duodenum.
  • Bacterial count in the mucous layer lining the intestinal walls was found to be closely related to the lumenal (interior) bacterial count, although actual values were lower in mucosal samples.

Comparison with the White Rhino

  • Although the comparison was limited to a single white rhino, the study found higher bacterial counts in the cecum rather than in the colon, similar to the findings in horses.

Cite This Article

APA
Mackie RI, Wilkins CA. (1988). Enumeration of anaerobic bacterial microflora of the equine gastrointestinal tract. Appl Environ Microbiol, 54(9), 2155-2160. https://doi.org/10.1128/aem.54.9.2155-2160.1988

Publication

Applied and environmental microbiology
ISSN: 0099-2240
NlmUniqueID: 7605801
Country: United States
Language: English
Volume: 54
Issue: 9
Pages: 2155-2160

Researcher Affiliations

Mackie, R I
  • Rumen Biochemistry, Animal & Dairy Science Research Institute, Irene, Republic of South Africa.
Wilkins, C A

    MeSH Terms

    • Animals
    • Bacteria / growth & development
    • Cecum / analysis
    • Cecum / microbiology
    • Colic / etiology
    • Colic / veterinary
    • Colon / analysis
    • Colon / microbiology
    • Colony Count, Microbial
    • Digestive System / analysis
    • Digestive System / microbiology
    • Duodenum / analysis
    • Duodenum / microbiology
    • Fatty Acids, Volatile / analysis
    • Female
    • Horse Diseases / etiology
    • Horses / microbiology
    • Hydrogen-Ion Concentration
    • Ileum / analysis
    • Ileum / microbiology
    • Intestinal Diseases / etiology
    • Intestinal Diseases / veterinary
    • Intestinal Mucosa / microbiology
    • Jejunum / analysis
    • Jejunum / microbiology
    • Male
    • Perissodactyla / microbiology

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    Citations

    This article has been cited 24 times.
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    2. Perricone V, Sandrini S, Irshad N, Comi M, Lecchi C, Savoini G, Agazzi A. The Role of Yeast Saccharomyces cerevisiae in Supporting Gut Health in Horses: An Updated Review on Its Effects on Digestibility and Intestinal and Fecal Microbiota.. Animals (Basel) 2022 Dec 9;12(24).
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    3. Froidurot A, Julliand V. Cellulolytic bacteria in the large intestine of mammals.. Gut Microbes 2022 Jan-Dec;14(1):2031694.
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