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Clinical and vaccine immunology : CVI2009; 16(5); 667-671; doi: 10.1128/CVI.00043-09

Enzyme-linked immunosorbent assay using glycoprotein and monoclonal antibody for detecting antibodies to vesicular stomatitis virus serotype New Jersey.

Abstract: In this study, an enzyme-linked immunosorbent assay (ELISA) using glycoprotein and a monoclonal antibody (MAb) was developed for the detection of antibodies to vesicular stomatitis virus (VSV) serotype New Jersey (NJ). The glycoprotein to be used as a diagnostic antigen was extracted from partially purified VSV-NJ, and a neutralizing MAb specific to VSV-NJ was incorporated to compete with antibodies in a blocking ELISA using glycoprotein (GP ELISA). The cutoff of the GP ELISA was set at 40% inhibition, which corresponded to a virus neutralization test (VNT) titer of 32. With this threshold, the GP ELISA exhibited 99.6% specificity for naïve sera (n = 3,005) from cattle (n = 1,040), pigs (n = 1,120), and horses (n = 845) from domestic farms. The GP ELISA did not cross-react with sera positive for foot-and-mouth disease virus, swine vesicular disease virus, or VSV serotype Indiana. The GP ELISA was more compatible with the VNT than was the nucleocapsid-based ELISA for VSV-NJ-positive sera (n = 19). Taken together, this GP ELISA could be a useful tool as an alternative to the VNT for detecting antibodies specific to VSV-NJ.
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Publication Date: 2009-03-11 PubMed ID: 19279165PubMed Central: PMC2681588DOI: 10.1128/CVI.00043-09Google Scholar: Lookup
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  • Evaluation Study
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research carefully developed and examined an enzyme-linked immunosorbent assay, making use of a glycoprotein and a monoclonal antibody, which can efficiently detect antibodies to vesicular stomatitis virus serotype New Jersey.

Research Methodology

  • The study focused on creating an enzyme-linked immunosorbent assay (ELISA) which employed a glycoprotein and a monoclonal antibody (MAb) to detect antibodies to vesicular stomatitis virus (VSV) serotype New Jersey (NJ).
  • The researchers extracted the required glycoprotein from partially purified VSV-NJ, which served as a diagnostic antigen.
  • A neutralizing MAb, specific to VSV-NJ, was used to compete with the antibodies in a blocking ELISA using the glycoprotein, known as the GP ELISA.

Observations and Findings

  • The cutoff for the GP ELISA was set at 40% inhibition, equivalent to a virus neutralization test (VNT) titer of 32.
  • At this cutoff, the GP ELISA demonstrated 99.6% specificity when tested on naïve sera (protein portion of blood) collected from a number of domesticated animals (n = 3,005) from different cattle, horses and pig farms.
  • The GP ELISA did not exhibit cross-reactivity with sera positive for other viruses like foot-and-mouth disease virus, swine vesicular disease virus, or VSV serotype Indiana.
  • The researchers observed that GP ELISA was more compatible with VNT than the nucleocapsid-based ELISA for VSV-NJ-positive sera (n = 19).

Conclusion

  • The study concludes that the developed GP ELISA is a useful tool and a reliable alternative to the VNT for detecting antibodies specific to VSV-NJ.

Cite This Article

APA
Lee HS, Heo EJ, Jeoung HY, Ko HR, Kweon CH, Youn HJ, Ko YJ. (2009). Enzyme-linked immunosorbent assay using glycoprotein and monoclonal antibody for detecting antibodies to vesicular stomatitis virus serotype New Jersey. Clin Vaccine Immunol, 16(5), 667-671. https://doi.org/10.1128/CVI.00043-09

Publication

Clinical and vaccine immunology : CVI
ISSN: 1556-679X
NlmUniqueID: 101252125
Country: United States
Language: English
Volume: 16
Issue: 5
Pages: 667-671

Researcher Affiliations

Lee, Hyang-Sim
  • National Veterinary Research and Quarantine Service, Anyang, Gyeonggi-do, Republic of Korea.
Heo, Eun-Jeong
    Jeoung, Hye-Young
      Ko, Hyo-Rim
        Kweon, Chang-Hee
          Youn, Hee-Jeong
            Ko, Young-Joon

              MeSH Terms

              • Animals
              • Antibodies
              • Antibodies, Monoclonal
              • Antibodies, Viral / blood
              • Cattle
              • Cattle Diseases / diagnosis
              • Cattle Diseases / virology
              • Enzyme-Linked Immunosorbent Assay / methods
              • Glycoproteins
              • Horse Diseases / diagnosis
              • Horse Diseases / virology
              • Horses
              • Rhabdoviridae Infections / diagnosis
              • Rhabdoviridae Infections / veterinary
              • Rhabdoviridae Infections / virology
              • Sensitivity and Specificity
              • Swine
              • Swine Diseases / diagnosis
              • Swine Diseases / virology
              • Vesicular stomatitis New Jersey virus / isolation & purification

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              Citations

              This article has been cited 1 times.
              1. Lin T, Shao J, Chang H, Gao S, Cong G, Du J. Generation of monoclonal antibodies against non-structural protein 3AB of foot-and-mouth disease virus. Virol Sin 2012 Oct;27(5):316-9.
                doi: 10.1007/s12250-012-3261-xpubmed: 23055007google scholar: lookup
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