FREE SHIPPING over $40
OVER 9000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Home / Equine Research Bank / J Vet Med Sci / Enzyme-linked immunosorbent assays using recombinant TgSAG2 ...
The Journal of veterinary medical science2016; 78(12); 1877-1881; doi: 10.1292/jvms.16-0234

Enzyme-linked immunosorbent assays using recombinant TgSAG2 and NcSAG1 to detect Toxoplasma gondii and Neospora caninum-specific antibodies in domestic animals in Turkey.

Abstract: Considering the scarce information on occurrences of Toxoplasma gondii and Neospora caninum in domestic animals from Turkey, the aim of this study was to investigate the seroprevalence of these parasite infections in cattle, horses, sheep, goats and dogs in Turkey. The specific antibodies against T. gondii and N. caninum were detected by iELISAs based on the recombinant TgSAG2 or NcSAG1 in a total of 2,039 serum samples from eleven provinces. The seroprevalence of T. gondii infections was 46.3%, 4.0%, 20.0%, 12.9% and 19.8%, that of N. caninum infections was 0.3%, 7.4%, 2.1%, 3.2% and 16.6% in the horses, cattle, sheep, goats and dogs, respectively. These results indicated that T. gondii and N. caninum infections are prevalent in Turkish domestic animals.
Get Full Text
Publication Date: 2016-08-15 PubMed ID: 27523504PubMed Central: PMC5240768DOI: 10.1292/jvms.16-0234Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research study investigated the prevalence of Toxoplasma gondii and Neospora caninum infections in domestic animals across eleven provinces in Turkey, using Enzyme-Linked Immunosorbent Assays (ELISAs) that feature recombinant TgSAG2 and NcSAG1.

Objective and Methodology of the Research

  • The main objective of this research was to assess the prevalence of Toxoplasma gondii and Neospora caninum infections in domestic animals in Turkey, due to scarce information on these occurrences.
  • It aimed at finding specific antibodies against T. gondii and N. caninum in serum samples collected from the animals.
  • The anticipated result was achieved via use of Enzyme-Linked Immunosorbent Assays (ELISAs) that applied the recombinant TgSAG2 or NcSAG1.
  • A total of 2,039 serum samples were evaluated from a assorted range of domestic animals including cattle, horses, sheep, goats, and dogs, over eleven provinces in Turkey.

Results from the Research

  • Results indicated a varying degree of seroprevalences with T. gondii infections being 46.3% in horses, 4.0% in cattle, 20.0% in sheep, 12.9% in goats, and 19.8% in dogs.
  • Meanwhile, the seroprevalence of N. caninum infections was found to be significantly lower in comparison: 0.3% in horses, 7.4% in cattle, 2.1% in sheep, 3.2% in goats, and 16.6% in dogs.

Conclusion from the Findings

  • These findings suggested that infections of T. gondii and N. caninum are indeed prevalent in domestic animals in Turkey, validating the need for this study.
  • A better understanding of the epidemiology of these infections in Turkey can serve as a basis for developing strategic interventions to control their spread and potentially prevent human infections.

Cite This Article

APA
Zhou M, Cao S, Sevinc F, Sevinc M, Ceylan O, Liu M, Wang G, Moumouni PF, Jirapattharasate C, Suzuki H, Nishikawa Y, Xuan X. (2016). Enzyme-linked immunosorbent assays using recombinant TgSAG2 and NcSAG1 to detect Toxoplasma gondii and Neospora caninum-specific antibodies in domestic animals in Turkey. J Vet Med Sci, 78(12), 1877-1881. https://doi.org/10.1292/jvms.16-0234

Publication

The Journal of veterinary medical science
ISSN: 1347-7439
NlmUniqueID: 9105360
Country: Japan
Language: English
Volume: 78
Issue: 12
Pages: 1877-1881

Researcher Affiliations

Zhou, Mo
  • National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan.
Cao, Shinuo
    Sevinc, Ferda
      Sevinc, Mutlu
        Ceylan, Onur
          Liu, Mingming
            Wang, Guanbo
              Moumouni, Paul Franck Adjou
                Jirapattharasate, Charoonluk
                  Suzuki, Hiroshi
                    Nishikawa, Yoshifumi
                      Xuan, Xuenan

                        MeSH Terms

                        • Animals
                        • Animals, Domestic / parasitology
                        • Antibodies, Protozoan / blood
                        • Antigens, Protozoan / immunology
                        • Dogs / parasitology
                        • Enzyme-Linked Immunosorbent Assay / veterinary
                        • Horses / blood
                        • Horses / parasitology
                        • Neospora / immunology
                        • Protozoan Proteins / immunology
                        • Ruminants / parasitology
                        • Seroepidemiologic Studies
                        • Toxoplasma / immunology
                        • Turkey

                        References

                        This article includes 29 references
                        1. Barber JS, Trees AJ. Clinical aspects of 27 cases of neosporosis in dogs.. Vet Rec 1996 Nov 2;139(18):439-43.
                          doi: 10.1136/vr.139.18.439pubmed: 8931299google scholar: lookup
                        2. Bártová E, Sedlak K. Toxoplasma gondii and Neospora caninum antibodies in goats in the Czech Republic.. Vet. Med. 57: 111–114.
                        3. Bártová E, Sedlák K, Literák I. Toxoplasma gondii and Neospora caninum antibodies in sheep in the Czech Republic.. Vet Parasitol 2009 Apr 6;161(1-2):131-2.
                          doi: 10.1016/j.vetpar.2008.12.022pubmed: 19181450google scholar: lookup
                        4. Cedillo-Peláez C, Díaz-Figueroa ID, Jiménez-Seres MI, Sánchez-Hernández G, Correa D. Frequency of antibodies to Toxoplasma gondii in stray dogs of Oaxaca, México.. J Parasitol 2012 Aug;98(4):871-2.
                          doi: 10.1645/GE-3095.1pubmed: 22360636google scholar: lookup
                        5. Chahan B, Gaturaga I, Huang X, Liao M, Fukumoto S, Hirata H, Nishikawa Y, Suzuki H, Sugimoto C, Nagasawa H, Fujisaki K, Igarashi I, Mikami T, Xuan X. Serodiagnosis of Neospora caninum infection in cattle by enzyme-linked immunosorbent assay with recombinant truncated NcSAG1.. Vet Parasitol 2003 Dec 30;118(3-4):177-85.
                          doi: 10.1016/j.vetpar.2003.10.010pubmed: 14729165google scholar: lookup
                        6. Diakoua A, Papadopoulos E, Panousis N, Karatzias C, Giadinis N. Toxoplasma gondii and Neospora caninum seroprevalence in dairy sheep and goats mixed stock farming.. Vet Parasitol 2013 Dec 6;198(3-4):387-90.
                          doi: 10.1016/j.vetpar.2013.09.017pubmed: 24103737google scholar: lookup
                        7. Dubey J P, Beattie C P. General biology.. p.220. In: Toxoplasmosis of animals and man. CRC Press, Boca Raton..
                        8. Dubey JP, Thulliez P. Persistence of tissue cysts in edible tissues of cattle fed Toxoplasma gondii oocysts.. Am J Vet Res 1993 Feb;54(2):270-3.
                          pubmed: 8430937
                        9. Dubey JP, Kerber CE, Granstrom DE. Serologic prevalence of Sarcocystis neurona, Toxoplasma gondii, and Neospora caninum in horses in Brazil.. J Am Vet Med Assoc 1999 Oct 1;215(7):970-2.
                          pubmed: 10511862
                        10. Dubey JP. Review of Neospora caninum and neosporosis in animals.. Korean J Parasitol 2003 Mar;41(1):1-16.
                          doi: 10.3347/kjp.2003.41.1.1pmc: PMC2717477pubmed: 12666725google scholar: lookup
                        11. Dubey JP, Mitchell SM, Morrow JK, Rhyan JC, Stewart LM, Granstrom DE, Romand S, Thulliez P, Saville WJ, Lindsay DS. Prevalence of antibodies to Neospora caninum, Sarcocystis neurona, and Toxoplasma gondii in wild horses from central Wyoming.. J Parasitol 2003 Aug;89(4):716-20.
                          doi: 10.1645/GE-66Rpubmed: 14533680google scholar: lookup
                        12. Faria EB, Gennari SM, Pena HF, Athayde AC, Silva ML, Azevedo SS. Prevalence of anti-Toxoplasma gondii and anti-Neospora caninum antibodies in goats slaughtered in the public slaughterhouse of Patos city, Paraíba State, Northeast region of Brazil.. Vet Parasitol 2007 Oct 21;149(1-2):126-9.
                          doi: 10.1016/j.vetpar.2007.07.009pubmed: 17706359google scholar: lookup
                        13. Hemphill A, Fuchs N, Sonda S, Gottstein B, Hentrich B. Identification and partial characterization of a 36 kDa surface protein on Neospora caninum tachyzoites.. Parasitology 1997 Oct;115 ( Pt 4):371-80.
                          doi: 10.1017/S0031182097001455pubmed: 9364563google scholar: lookup
                        14. Hosseini M, Moraveji M, Tahamtan Y, Rahimian A, Mohammadi G, Namavari M. Seroprevalence of Neospora spp. in Horses in North East of Iran.. Iran J Parasitol 2011 Jun;6(2):64-8.
                          pmc: PMC3279880pubmed: 22347290
                        15. Howe DK, Crawford AC, Lindsay D, Sibley LD. The p29 and p35 immunodominant antigens of Neospora caninum tachyzoites are homologous to the family of surface antigens of Toxoplasma gondii.. Infect Immun 1998 Nov;66(11):5322-8.
                          pmc: PMC108665pubmed: 9784539doi: 10.1128/iai.66.11.5322-5328.1998google scholar: lookup
                        16. Huang X, Xuan X, Kimbita EN, Battur B, Miyazawa T, Fukumoto S, Mishima M, Makala LH, Suzuki H, Sugimoto C, Nagasawa H, Fujisaki K, Mikami T, Igarashi I. Development and evaluation of an enzyme-linked immunosorbent assay with recombinant SAG2 for diagnosis of Toxoplasma gondii infection in cats.. J Parasitol 2002 Aug;88(4):804-7.
                          doi: 10.1645/0022-3395(2002)088[0804:DAEOAE]2.0.CO;2pubmed: 12197139google scholar: lookup
                        17. Ibrahim HM, Huang P, Salem TA, Talaat RM, Nasr MI, Xuan X, Nishikawa Y. Short report: prevalence of Neospora caninum and Toxoplasma gondii antibodies in northern Egypt.. Am J Trop Med Hyg 2009 Feb;80(2):263-7.
                          pubmed: 19190225
                        18. Kamani J, Mani AU, Kumshe HA, Dogo GI, Yidawi JP, Pauline DK, Nnabuife HE, Joan P, Egwu GO. Serosurvey for Toxoplasma gondii in dogs in Maiduguri, Borno State, Nigeria.. J Infect Dev Ctries 2009 Nov 21;4(1):15-8.
                          pubmed: 20130373doi: 10.3855/jidc.428google scholar: lookup
                        19. Oncel T, Vural G. Occurrence of Toxoplasma gondii antibodies in sheep in Istanbul, Turkey.. Vet. Arh. 76: 547–553.
                        20. Pita Gondim LF, Barbosa HV Jr, Ribeiro Filho CH, Saeki H. Serological survey of antibodies to Toxoplasma gondii in goats, sheep, cattle and water buffaloes in Bahia State, Brazil.. Vet Parasitol 1999 May;82(4):273-6.
                          doi: 10.1016/S0304-4017(99)00033-3pubmed: 10384902google scholar: lookup
                        21. Prince JB, Auer KL, Huskinson J, Parmley SF, Araujo FG, Remington JS. Cloning, expression, and cDNA sequence of surface antigen P22 from Toxoplasma gondii.. Mol Biochem Parasitol 1990 Nov;43(1):97-106.
                          doi: 10.1016/0166-6851(90)90134-8pubmed: 2290448google scholar: lookup
                        22. Raeghi S, Akaberi A, Sedeghi S. Seroprevalence of Toxoplasma gondii in Sheep, Cattle and Horses in Urmia North-West of Iran.. Iran J Parasitol 2011 Dec;6(4):90-4.
                          pmc: PMC3279903pubmed: 22347318
                        23. Reid AJ, Vermont SJ, Cotton JA, Harris D, Hill-Cawthorne GA, Könen-Waisman S, Latham SM, Mourier T, Norton R, Quail MA, Sanders M, Shanmugam D, Sohal A, Wasmuth JD, Brunk B, Grigg ME, Howard JC, Parkinson J, Roos DS, Trees AJ, Berriman M, Pain A, Wastling JM. Comparative genomics of the apicomplexan parasites Toxoplasma gondii and Neospora caninum: Coccidia differing in host range and transmission strategy.. PLoS Pathog 2012;8(3):e1002567.
                          doi: 10.1371/journal.ppat.1002567pmc: PMC3310773pubmed: 22457617google scholar: lookup
                        24. Salaberry SR, Okuda LH, Nassar AF, de Castro JR, Lima-Ribeiro AM. Prevalence of Neospora caninum antibodies in sheep flocks of Uberlândia county, MG.. Rev Bras Parasitol Vet 2010 Jul-Sep;19(3):148-51.
                          doi: 10.1590/S1984-29612010000300004pubmed: 20943017google scholar: lookup
                        25. Tassi P. Toxoplasma gondii infection in horses. A review.. Parassitologia 2007 Jun;49(1-2):7-15.
                          pubmed: 18412038
                        26. Trees AJ, Davison HC, Innes EA, Wastling JM. Towards evaluating the economic impact of bovine neosporosis.. Int J Parasitol 1999 Aug;29(8):1195-200.
                          doi: 10.1016/S0020-7519(99)00093-4pubmed: 10576571google scholar: lookup
                        27. Wanha K, Edelhofer R, Gabler-Eduardo C, Prosl H. Prevalence of antibodies against Neospora caninum and Toxoplasma gondii in dogs and foxes in Austria.. Vet Parasitol 2005 Mar 31;128(3-4):189-93.
                          doi: 10.1016/j.vetpar.2004.11.027pubmed: 15740855google scholar: lookup
                        28. Webster J P. Review of “Toxoplasmosis of Animals and Humans (Second Edition)” by J. P. Dubey.. Parasites & Vectors 3: 112.
                        29. Yan C, Fu LL, Yue CL, Tang RX, Liu YS, Lv L, Shi N, Zeng P, Zhang P, Wang DH, Zhou DH, Zhu XQ, Zheng KY. Stray dogs as indicators of Toxoplasma gondii distributed in the environment: the first report across an urban-rural gradient in China.. Parasit Vectors 2012 Jan 5;5:5.
                          doi: 10.1186/1756-3305-5-5pmc: PMC3271967pubmed: 22217112google scholar: lookup

                        Citations

                        This article has been cited 20 times.
                        1. Holec-Gąsior L, Sołowińska K. Detection of Toxoplasma gondii Infection in Small Ruminants: Old Problems, and Current Solutions. Animals (Basel) 2023 Aug 23;13(17).
                          doi: 10.3390/ani13172696pubmed: 37684960google scholar: lookup
                        2. Hu XH, Xie SC, Liang QL, Sun LX, Li Z, Yang JF, Zhu XQ, Zou FC, He JJ. Seroprevalence and risk factors of Toxoplasma gondii and Neospora caninum infection in black goats in Yunnan Province, Southwestern China. Front Vet Sci 2022;9:975238.
                          doi: 10.3389/fvets.2022.975238pubmed: 36304411google scholar: lookup
                        3. Benavides J, González-Warleta M, Arteche-Villasol N, Pérez V, Mezo M, Gutiérrez-Expósito D. Ovine Neosporosis: The Current Global Situation. Animals (Basel) 2022 Aug 15;12(16).
                          doi: 10.3390/ani12162074pubmed: 36009665google scholar: lookup
                        4. Qi T, Ai J, Yang J, Zhu H, Zhou Y, Zhu Y, Zhang H, Qin Q, Kang M, Sun Y, Li J. Seroepidemiology of Neosporosis in Various Animals in the Qinghai-Tibetan Plateau. Front Vet Sci 2022;9:953380.
                          doi: 10.3389/fvets.2022.953380pubmed: 35928116google scholar: lookup
                        5. Yang J, Ai J, Qi T, Ni X, Xu Z, Guo L, Sun Y, Li Y, Kang M, Li J. Toxoplasma gondii and Neospora caninum Infections in Stray Cats and Dogs in the Qinghai-Tibetan Plateau Area, China. Animals (Basel) 2022 May 28;12(11).
                          doi: 10.3390/ani12111390pubmed: 35681854google scholar: lookup
                        6. Hajimohammadi B, Ahmadian S, Firoozi Z, Askari M, Mohammadi M, Eslami G, Askari V, Loni E, Barzegar-Bafrouei R, Boozhmehrani MJ. A Meta-Analysis of the Prevalence of Toxoplasmosis in Livestock and Poultry Worldwide. Ecohealth 2022 Mar;19(1):55-74.
                          doi: 10.1007/s10393-022-01575-xpubmed: 35133541google scholar: lookup
                        7. Li G, Zheng W, Yang J, Qi T, He Y, Chen W, Ma H, Sun Y, Li Y, Kang M, Li J. Seroprevalence and Epidemiology of Toxoplasma gondii in Animals in the Qinghai-Tibetan Plateau Area, China. Pathogens 2021 Apr 6;10(4).
                          doi: 10.3390/pathogens10040432pubmed: 33917355google scholar: lookup
                        8. Watanabe M, Sadiq MB, Mulop NIA, Mohammed K, Rani PAM, Fong LS, Aziz NA, Kamaludeen J, Ramanoon SZ, Mansor R, Ping TL, Syed-Hussain SS. Prevalence of Toxoplasma gondii Antibodies in Stray Dogs from Various Locations in West and East Malaysia. Korean J Parasitol 2020 Oct;58(5):487-492.
                          doi: 10.3347/kjp.2020.58.5.487pubmed: 33202500google scholar: lookup
                        9. Ferra B, Holec-Gąsior L, Grąźlewska W. Toxoplasma gondii Recombinant Antigens in the Serodiagnosis of Toxoplasmosis in Domestic and Farm Animals. Animals (Basel) 2020 Jul 22;10(8).
                          doi: 10.3390/ani10081245pubmed: 32707821google scholar: lookup
                        10. Abdul Hamid N, Sadiq MB, Ramanoon SZ, Mansor R, Watanabe M, Md Isa NM, Kamaludeen J, Syed-Hussain SS. Seroprevalence and Risk Factors of Toxoplasma gondii in Ruminant Meats from Wet Markets in Klang Valley and Abattoirs in Selangor, Malaysia. Animals (Basel) 2020 Jul 6;10(7).
                          doi: 10.3390/ani10071139pubmed: 32640507google scholar: lookup
                        11. Winzer P, Müller J, Imhof D, Ritler D, Uldry AC, Braga-Lagache S, Heller M, Ojo KK, Van Voorhis WC, Ortega-Mora LM, Hemphill A. Neospora caninum: Differential Proteome of Multinucleated Complexes Induced by the Bumped Kinase Inhibitor BKI-1294. Microorganisms 2020 May 26;8(6).
                          doi: 10.3390/microorganisms8060801pubmed: 32466554google scholar: lookup
                        12. Stelzer S, Basso W, Benavides Silván J, Ortega-Mora LM, Maksimov P, Gethmann J, Conraths FJ, Schares G. Toxoplasma gondii infection and toxoplasmosis in farm animals: Risk factors and economic impact. Food Waterborne Parasitol 2019 Jun;15:e00037.
                          doi: 10.1016/j.fawpar.2019.e00037pubmed: 32095611google scholar: lookup
                        13. Kolören Z, Dubey JP. A review of toxoplasmosis in humans and animals in Turkey. Parasitology 2020 Jan;147(1):12-28.
                          doi: 10.1017/S0031182019001318pubmed: 31554526google scholar: lookup
                        14. Gareh A, Elbarbary NK, Fotouh A, Maher A, Mohamed YM, Ragab MF. Seromolecular and histopathological study on Toxoplasma gondii infection in ruminants in Aswan, Egypt. BMC Vet Res 2025 Dec 30;22(1):49.
                          doi: 10.1186/s12917-025-05195-9pubmed: 41469874google scholar: lookup
                        15. Ceylan O, Sevinc F, Bonkoungou AB, Ceylan C, Yonar H, Ghaibullah MN, Cao S, Xuan X. Nationwide enzooticity of ovine toxoplasmosis and neosporosis in Türkiye. BMC Microbiol 2025 Nov 27;25(1):783.
                          doi: 10.1186/s12866-025-04534-ypubmed: 41310479google scholar: lookup
                        16. Jabar Aziz K, Mikaeel FB, Azeez SH, Bilal SJ. Seroepidemiological study of Neospora caninum in equids using c-ELISA in Erbil Province, Iraq. Folia Parasitol 2024 Nov 25;71.
                          doi: 10.14411/fp.2024.022pubmed: 39632700google scholar: lookup
                        17. Morganti G, Rigamonti G, Brustenga L, Calgaro V, Angeli G, Moretta I, Diaferia M, Veronesi F. Exploring similarities and differences between Toxoplasma gondii and Neospora caninum infections in dogs. Vet Res Commun 2024 Dec;48(6):3563-3577.
                          doi: 10.1007/s11259-024-10549-zpubmed: 39320405google scholar: lookup
                        18. Ferra BT, Chyb M, Sołowińska K, Holec-Gąsior L, Skwarecka M, Baranowicz K, Gatkowska J. The Development of Toxoplasma gondii Recombinant Trivalent Chimeric Proteins as an Alternative to Toxoplasma Lysate Antigen (TLA) in Enzyme-Linked Immunosorbent Assay (ELISA) for the Detection of Immunoglobulin G (IgG) in Small Ruminants. Int J Mol Sci 2024 Apr 16;25(8).
                          doi: 10.3390/ijms25084384pubmed: 38673969google scholar: lookup
                        19. Turlewicz-Podbielska H, Ruszkowski JJ, Wojciechowski J, Pomorska-Mól M. Seroprevalence of Neospora caninum in pet cats, dogs and rabbits from urban areas of Poland. BMC Vet Res 2024 Jan 31;20(1):37.
                          doi: 10.1186/s12917-024-03894-3pubmed: 38297328google scholar: lookup
                        20. Shariatzadeh SA, Sarvi S, Hosseini SA, Sharif M, Gholami S, Pagheh AS, Montazeri F, Nayeri T, Nakhaei M, Mikaeili Galeh T, Daryani A. The global seroprevalence of Toxoplasma gondii infection in bovines: a systematic review and meta-analysis. Parasitology 2021 Oct;148(12):1417-1433.
                          doi: 10.1017/S0031182021001116pubmed: 34187606google scholar: lookup
                        Subscribe to our newsletter
                        Join 99,357 horse owners like you who receive our email updates on horse health and nutrition.