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Journal of applied genetics2014; 56(2); 231-238; doi: 10.1007/s13353-014-0244-7

Epigenetic structure and the role of polymorphism in the shaping of DNA methylation patterns of equine OAS1 locus.

Abstract: DNA methylation patterns and their relation with genetic polymorphisms were determined in the equine OAS1 locus. Genetic variants of OAS1 were previously found to be associated with susceptibility to West Nile virus infections in horses. The subject of the study were white blood cells of 13 juvenile and 13 old horses from AA and HC breed and a set of solid tissues from a single adult horse. The aim was to determine the degree of variation of CpG methylation profiles with concern for tissue type, horse breed and age. Results of direct BSPCR and cloned BSPCR sequencing revealed that all of determined CpG islands (CGIs) were hypermethylated in exception to CGI covering OAS1 promoter and exon 1. One of intragenic CGIs displayed variability of methylation patterns across eight tissue types. The variability of particular sub-types of white blood cells between AA and HC horses were considered as the possible cause of interbreed differences of methylation levels. Comparison of sequence variability between converted and unconverted DNAs of both horse breeds showed polymorphisms of CpG sites to be the source of monoallelic methylation in exception to the polymorphic CpGs located in the OAS1 promoter. Two of them are new polymorphic variants in the OAS1 promoter region. Application of methylation data in conjunction with genetic variation detected at the OAS1 locus might be useful to deepen the knowledge about mechanisms underlying immunity to viral infections in the horse.
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Publication Date: 2014-09-07 PubMed ID: 25195205DOI: 10.1007/s13353-014-0244-7Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The researchers explored the relationship between the patterns of DNA methylation and genetic variances in the equine OAS1 gene, which has previously been linked to susceptibility to West Nile virus in horses.

Method

  • The study involved white blood cells from 13 young and 13 old horses of two breeds: AA and HC, along with tissue samples from a single adult horse.
  • The team aimed to identify any variations in the methylation profiles of CpG (regions of DNA where a cytosine nucleotide is located next to a guanine nucleotide) concerning the type of tissue, the breed of horse, and age.

Findings

  • Through the use of direct BSPCR (Bisulfite Polymerase Chain Reaction) and cloned BSPCR sequencing, the researchers found that all the CpG islands (regions with a high frequency of CpG sites) were heavily methylated. A noticeable exception was the CpG island covering the OAS1 promoter and exon 1.
  • One of the CGIs within the OAS1 gene displayed variation in its methylation patterns across eight different tissue types.
  • There were differences in the methylation levels between the AA and HC horse breeds, speculated to be potentially due to variability in specific sub-types of white blood cells.

Impact of Genetic Polymorphisms

  • Comparison of sequence variability between converted and unconverted DNAs of both horse breeds showed polymorphisms of CpG sites to be the cause of monoallelic methylation, with the exception of the polymorphic CpGs located in the OAS1 promoter.
  • Two new polymorphic variants were discovered in the OAS1 promoter region.

Implications

  • The findings suggest that applying methylation data and the genetic variation detected at the OAS1 locus could be beneficial for understanding the mechanisms underlying immunity to viral infections in horses.

Cite This Article

APA
Ząbek T, Semik E, Wnuk M, Fornal A, Gurgul A, Bugno-Poniewierska M. (2014). Epigenetic structure and the role of polymorphism in the shaping of DNA methylation patterns of equine OAS1 locus. J Appl Genet, 56(2), 231-238. https://doi.org/10.1007/s13353-014-0244-7

Publication

Journal of applied genetics
ISSN: 2190-3883
NlmUniqueID: 9514582
Country: England
Language: English
Volume: 56
Issue: 2
Pages: 231-238

Researcher Affiliations

Ząbek, T
  • National Research, Institute of Animal Production, Krakowska 1, 32-083, Balice, Poland, tomasz.zabek@izoo.krakow.pl.
Semik, E
    Wnuk, M
      Fornal, A
        Gurgul, A
          Bugno-Poniewierska, M

            MeSH Terms

            • 2',5'-Oligoadenylate Synthetase / genetics
            • Animals
            • Breeding
            • CpG Islands
            • DNA Methylation
            • Disease Susceptibility
            • Epigenesis, Genetic
            • Horses / genetics
            • Male
            • Polymorphism, Genetic
            • Promoter Regions, Genetic
            • West Nile Fever / veterinary

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            Citations

            This article has been cited 1 times.
            1. Bhardwaj A, Tandon G, Pal Y, Sharma NK, Nayan V, Soni S, Iquebal MA, Jaiswal S, Legha RA, Talluri TR, Bhattacharya TK, Kumar D, Rai A, Tripathi BN. Genome-Wide Single-Nucleotide Polymorphism-Based Genomic Diversity and Runs of Homozygosity for Selection Signatures in Equine Breeds.. Genes (Basel) 2023 Aug 14;14(8).
              doi: 10.3390/genes14081623pubmed: 37628674google scholar: lookup
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