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Home / Equine Research Bank / J Clin Microbiol / Epitope-blocking enzyme-linked immunosorbent assays for dete...
Journal of clinical microbiology2003; 41(6); 2676-2679; doi: 10.1128/JCM.41.6.2676-2679.2003

Epitope-blocking enzyme-linked immunosorbent assays for detection of west nile virus antibodies in domestic mammals.

Abstract: We evaluated the ability of epitope-blocking enzyme-linked immunosorbent assays (ELISAs) to detect West Nile virus (WNV) antibodies in domestic mammals. Sera were collected from experimentally infected horses, cats, and pigs at regular intervals and screened in ELISAs and plaque reduction neutralization tests. The diagnostic efficacies of these techniques were similar.
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Publication Date: 2003-06-07 PubMed ID: 12791902PubMed Central: PMC156482DOI: 10.1128/JCM.41.6.2676-2679.2003Google Scholar: Lookup
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  • Evaluation Study
  • Journal Article
  • Research Support
  • U.S. Gov't
  • P.H.S.

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research study is about testing the effectiveness of epitope-blocking enzyme-linked immunosorbent assays (ELISAs) in identifying West Nile virus (WNV) antibodies in animals like horses, cats, and pigs. It was found that their diagnostic capabilities are much like those of plaque reduction neutralization tests.

Introduction to the Research

  • The research primarily focused on the application and effectiveness of epitope-blocking enzyme-linked immunosorbent assays (ELISAs) in the detection of West Nile virus (WNV) in domestic mammals.
  • The study was carried out on animals, specifically horses, cats, and pigs, which were infected experimentally in a controlled environment.

Procedure of the Experiment

  • In the procedure, sera or blood from these infected animals were collected regularly at different intervals.
  • The collected sera were then subjected to both epitope-blocking ELISAs and plaque reduction neutralization tests for comparison.

Results of the Study

  • The results of the study indicated that the diagnostic efficacy of epitope-blocking ELISAs was similar to that of plaque reduction neutralization tests.
  • This suggests that epitope-blocking ELISAs can be a reliable method for detecting WNV in domestic mammals.

Impact of the Findings

  • The findings of the study could have significant implications in the field of virology, particularly in the diagnosis of WNV.
  • Understanding the utility of epitope-blocking ELISAs in accurately identifying WNV antibodies could pave the way for streamlined diagnosis and thus quicker treatment of the disease in domestic and potentially other animals.

Cite This Article

APA
Blitvich BJ, Bowen RA, Marlenee NL, Hall RA, Bunning ML, Beaty BJ. (2003). Epitope-blocking enzyme-linked immunosorbent assays for detection of west nile virus antibodies in domestic mammals. J Clin Microbiol, 41(6), 2676-2679. https://doi.org/10.1128/JCM.41.6.2676-2679.2003

Publication

Journal of clinical microbiology
ISSN: 0095-1137
NlmUniqueID: 7505564
Country: United States
Language: English
Volume: 41
Issue: 6
Pages: 2676-2679

Researcher Affiliations

Blitvich, Bradley J
  • Arthropod-Borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523, USA.
Bowen, Richard A
    Marlenee, Nicole L
      Hall, Roy A
        Bunning, Michel L
          Beaty, Barry J

            MeSH Terms

            • Animals
            • Animals, Domestic / virology
            • Antibodies, Monoclonal / immunology
            • Antibodies, Viral / blood
            • Cat Diseases / virology
            • Cats
            • Enzyme-Linked Immunosorbent Assay / methods
            • Epitopes / immunology
            • Horse Diseases / virology
            • Horses
            • Neutralization Tests
            • Swine
            • Swine Diseases / virology
            • Viral Plaque Assay
            • West Nile Fever / diagnosis
            • West Nile Fever / veterinary
            • West Nile virus / immunology

            Grant Funding

            • U50 CCU820510 / ODCDC CDC HHS

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