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Applied microbiology1972; 23(6); 1121-1124; doi: 10.1128/am.23.6.1121-1124.1972

Equine abortion (herpes) virus: strain differences in susceptibility to inactivation by dithiothreitol.

Abstract: The infectivity of equine abortion (herpes) virus (EAV) was inactivated by treatment with reduced dithiothreitol (DTT). According to their susceptibility to DTT, the EAV strains could be divided into three groups. The vaccine strain RAC-H (419) proved to be more resistant to DTT than all of the other 14 strains tested. The hemagglutinin of EAV was also inactivated by DTT; no strain differences were observed in this respect.
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Publication Date: 1972-06-01 PubMed ID: 4339509PubMed Central: PMC380517DOI: 10.1128/am.23.6.1121-1124.1972Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research examines how the infectivity of different strains of equine abortion (herpes) virus (EAV) is affected by treatment with reduced dithiothreitol (DTT). The findings show that a vaccine strain called RAC-H (419) is more resistant to DTT than 14 other strains tested, and that the hemagglutinin of EAV is also inactivated by DTT, with no observed strain differences.

Equine Abortion Virus and Dithiothreitol (DTT) Treatment

  • The study focuses on the effects of dithiothreitol (DTT), a reducing agent, on the infectivity of various strains of equine abortion (herpes) virus (EAV).
  • Equine abortion virus is a herpes virus which causes abortion in horses. Its strain differences and their response to treatments could have significant implications for the development of effective vaccines.
  • DTT is a chemical compound that is used in many biochemical applications to reduce disulphide bonds within and between proteins, altering their structural conformation and potentially affecting their functionality.

Strain Differences in DTT Susceptibility

  • The research found that the susceptibility of EAV to DTT varies across different strains, grouping them into three categories based on how readily they were inactivated.
  • Interestingly, the vaccine strain RAC-H (419) proved to be more resistant to DTT than all of the other 14 strains that were tested.
  • This suggests that the RAC-H (419) strain might have different structural properties, chemical bonds or protein arrangements that make it less susceptible to DTT’s effects.

Effect of DTT on Hemagglutinin

  • The hemagglutinin of EAV was also found to be inactivated by DTT, again pointing to DTT’s potential to disrupt the virus and its components.
  • However, interestingly, no strain differences in susceptibility to DTT were observed when it came to hemagglutinin inactivation.
  • This indicates that, although the overall susceptibility of EAV strains to DTT varies, the specific susceptibility of the hemagglutinin protein seems to be consistent across strains.

Cite This Article

APA
Klingeborn B, Dinter Z. (1972). Equine abortion (herpes) virus: strain differences in susceptibility to inactivation by dithiothreitol. Appl Microbiol, 23(6), 1121-1124. https://doi.org/10.1128/am.23.6.1121-1124.1972

Publication

Applied microbiology
ISSN: 0003-6919
NlmUniqueID: 7605802
Country: United States
Language: English
Volume: 23
Issue: 6
Pages: 1121-1124

Researcher Affiliations

Klingeborn, B
    Dinter, Z

      MeSH Terms

      • Abortion, Veterinary
      • Animals
      • Cattle
      • Cell Line / microbiology
      • Clone Cells
      • Dithiothreitol / pharmacology
      • Drug Resistance, Microbial
      • Female
      • Hemagglutination Tests
      • Hemagglutinins, Viral / antagonists & inhibitors
      • Herpesviridae / drug effects
      • Herpesviridae / growth & development
      • Herpesviridae / immunology
      • Herpesviridae Infections / veterinary
      • Horse Diseases
      • Horses
      • Kidney
      • L Cells / microbiology
      • Lung
      • Mice
      • Orthomyxoviridae Infections / veterinary
      • Pregnancy
      • Swine
      • Viral Vaccines
      • Virus Cultivation

      References

      This article includes 10 references
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        pubmed: 5692868
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        pubmed: 13302651
      9. DIDERHOLM H, STENKVIST B, PONTEN J, WESSLEN T. TRANSFORMATION OF BOVINE CELLS IN VITRO AFTER INOCULATION OF SIMIAN VIRUS 40 OR ITS NUCLEIC ACID.. Exp Cell Res 1965 Feb;37:452-9.
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      Citations

      This article has been cited 2 times.
      1. Klingeborn B. Equine abortion (herpes) virus: evaluation of markers in a field vaccination trial. Appl Microbiol 1973 Oct;26(4):566-9.
        doi: 10.1128/am.26.4.566-569.1973pubmed: 4796167google scholar: lookup
      2. Chowdhury SI, Kubin G, Ludwig H. Equine herpesvirus type 1 (EHV-1) induced abortions and paralysis in a Lipizzaner stud: a contribution to the classification of equine herpesviruses. Arch Virol 1986;90(3-4):273-88.
        doi: 10.1007/BF01317376pubmed: 3015084google scholar: lookup
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