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Home / Equine Research Bank / Stem Cells Int / Equine Adipose-Derived Mesenchymal Stromal Cells Release Ext...
Stem cells international2019; 2019; 4957806; doi: 10.1155/2019/4957806

Equine Adipose-Derived Mesenchymal Stromal Cells Release Extracellular Vesicles Enclosing Different Subsets of Small RNAs.

Abstract: Equine adipose-derived mesenchymal stromal cells (e-AdMSC) exhibit attractive proregenerative properties strongly related to the delivery of extracellular vesicles (EVs) that enclose different kinds of molecules including RNAs. In this study, we investigated small RNA content of EVs produced by e-AdMSC with the aim of speculating on their possible biological role. Methods: EVs were obtained by ultracentrifugation of the conditioned medium of e-AdMSC of 4 subjects. Transmission electron microscopy and scanning electron microscopy were performed to assess their size and nanostructure. RNA was isolated, enriched for small RNAs (<200 nt), and sequenced by Illumina technology. After bioinformatic analysis with state-of-the-art pipelines for short sequences, mapped reads were used to describe EV RNA cargo, reporting classes, and abundances. Enrichment analyses were performed to infer involved pathways and functional categories. Results: Electron microscopy showed the presence of vesicles ranging in size from 30 to 300 nm and expressing typical markers. RNA analysis revealed that ribosomal RNA was the most abundant fraction, followed by small nucleolar RNAs (snoRNAs, 13.67%). Miscellaneous RNA (misc_RNA) reached 4.57% of the total where Y RNA, RNaseP, and vault RNA represented the main categories. miRNAs were sequenced at a lower level (3.51%) as well as protein-coding genes (1.33%). Pathway analyses on the protein-coding fraction revealed a significant enrichment for the "ribosome" pathway followed by "oxidative phosphorylation." Gene Ontology analysis showed enrichment for terms like "extracellular exosome," "organelle envelope," "RNA binding," and "small molecule metabolic process." The miRNA target pathway analysis revealed the presence of "signaling pathways regulating pluripotency of stem cells" coherent with the source of the samples. Conclusions: We herein demonstrated that e-AdMSC release EVs enclosing different subsets of small RNAs that potentially regulate a number of biological processes. These findings shed light on the role of EVs in the context of MSC biology.
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Publication Date: 2019-03-18 PubMed ID: 31011332PubMed Central: PMC6442443DOI: 10.1155/2019/4957806Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research explores the small RNA content of extracellular vesicles (EVs) produced by Equine adipose-derived mesenchymal stromal cells, aiming to understand their possible biological role. The study results show these EVs contain different subsets of small RNAs that could potentially regulate numerous biological processes, providing further insights into the role of EVs in MSC biology.

Research Methodology

  • The researchers applied ultracentrifugation on the conditioned medium of e-AdMSC taken from four different subjects to acquire extracellular vesicles (EVs).
  • Two types of electron microscopy, transmission electron microscopy and scanning electron microscopy, were conducted to assess the size and the nano-structural details of the EVs.
  • The team isolated RNA from the EVs and filtered for small RNAs less than 200 nucleotides, which were then sequenced using Illumina technology.
  • A bioinformatic analysis was then carried out on the sequenced data, specifically focusing on the short sequences.
  • The classes and abundances of the mapped reads were used to describe the RNA cargo of the EVs. Additionally, enrichment analyses were carried out to discern the involved pathways and functional categories.

Research Findings

  • Electron microscopy revealed the presence of vesicles ranging in size from 30 to 300 nanometres, which express typical markers.
  • The researchers found that the most abundant fraction of RNA was ribosomal RNA. Small nucleolar RNAs made up 13.67%, and Miscellaneous RNA constituted 4.57% of the total. Within the misc_RNA section, Y RNA, RNaseP, and vault RNA were the main categories.
  • MicroRNAs were sequenced at a lower level (3.51%), in addition to protein-coding genes (1.33%).

Inferred Pathways and Functional Categories

  • The pathway analyses on the protein-coding fraction revealed significant enrichment for the “ribosome” pathway, which was followed by “oxidative phosphorylation.”
  • The Gene Ontology analysis showed enrichment for several terms, such as “extracellular exosome,” “organelle envelope,” “RNA binding,” and “small molecule metabolic process.”
  • MicroRNA target pathway analysis revealed the presence of “signaling pathways regulating pluripotency of stem cells,” aligning with the source of the samples.
  • These results indicate that e-AdMSC release EVs with different subsets of small RNAs which could potentially regulate a range of biological processes.

Implications and Conclusions

  • This study expands our understanding of the role of EVs in the functionality of MSC biology.
  • Recognizing the broad spectrum of small RNAs present in the extracellular vesicles can help to comprehend their function and involvement in various biological processes better.
  • The results have potential implications on how these small RNAs can be potentially used for therapeutic advancements, particularly in the field of regenerative medicine.

Cite This Article

APA
Capomaccio S, Cappelli K, Bazzucchi C, Coletti M, Gialletti R, Moriconi F, Passamonti F, Pepe M, Petrini S, Mecocci S, Silvestrelli M, Pascucci L. (2019). Equine Adipose-Derived Mesenchymal Stromal Cells Release Extracellular Vesicles Enclosing Different Subsets of Small RNAs. Stem Cells Int, 2019, 4957806. https://doi.org/10.1155/2019/4957806

Publication

Stem cells international
ISSN: 1687-966X
NlmUniqueID: 101535822
Country: United States
Language: English
Volume: 2019
Pages: 4957806
PII: 4957806

Researcher Affiliations

Capomaccio, Stefano
  • Dipartimento di Medicina Veterinaria, Università degli Studi di Perugia, Via San Costanzo, 4, 06126 Perugia, Italy.
  • Centro di Ricerca sul Cavallo Sportivo (CRCS), Università degli Studi di Perugia, Italy.
Cappelli, Katia
  • Dipartimento di Medicina Veterinaria, Università degli Studi di Perugia, Via San Costanzo, 4, 06126 Perugia, Italy.
  • Centro di Ricerca sul Cavallo Sportivo (CRCS), Università degli Studi di Perugia, Italy.
Bazzucchi, Cinzia
  • Dipartimento di Medicina Veterinaria, Università degli Studi di Perugia, Via San Costanzo, 4, 06126 Perugia, Italy.
Coletti, Mauro
  • Dipartimento di Medicina Veterinaria, Università degli Studi di Perugia, Via San Costanzo, 4, 06126 Perugia, Italy.
  • Centro di Ricerca sul Cavallo Sportivo (CRCS), Università degli Studi di Perugia, Italy.
Gialletti, Rodolfo
  • Dipartimento di Medicina Veterinaria, Università degli Studi di Perugia, Via San Costanzo, 4, 06126 Perugia, Italy.
  • Centro di Ricerca sul Cavallo Sportivo (CRCS), Università degli Studi di Perugia, Italy.
Moriconi, Franco
  • Dipartimento di Medicina Veterinaria, Università degli Studi di Perugia, Via San Costanzo, 4, 06126 Perugia, Italy.
  • Centro di Ricerca sul Cavallo Sportivo (CRCS), Università degli Studi di Perugia, Italy.
Passamonti, Fabrizio
  • Dipartimento di Medicina Veterinaria, Università degli Studi di Perugia, Via San Costanzo, 4, 06126 Perugia, Italy.
  • Centro di Ricerca sul Cavallo Sportivo (CRCS), Università degli Studi di Perugia, Italy.
Pepe, Marco
  • Dipartimento di Medicina Veterinaria, Università degli Studi di Perugia, Via San Costanzo, 4, 06126 Perugia, Italy.
  • Centro di Ricerca sul Cavallo Sportivo (CRCS), Università degli Studi di Perugia, Italy.
Petrini, Stefano
  • Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche, Italy.
Mecocci, Samanta
  • Dipartimento di Medicina Veterinaria, Università degli Studi di Perugia, Via San Costanzo, 4, 06126 Perugia, Italy.
  • Centro di Ricerca sul Cavallo Sportivo (CRCS), Università degli Studi di Perugia, Italy.
Silvestrelli, Maurizio
  • Dipartimento di Medicina Veterinaria, Università degli Studi di Perugia, Via San Costanzo, 4, 06126 Perugia, Italy.
  • Centro di Ricerca sul Cavallo Sportivo (CRCS), Università degli Studi di Perugia, Italy.
Pascucci, Luisa
  • Dipartimento di Medicina Veterinaria, Università degli Studi di Perugia, Via San Costanzo, 4, 06126 Perugia, Italy.
  • Centro di Ricerca sul Cavallo Sportivo (CRCS), Università degli Studi di Perugia, Italy.

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