Equine adipose-derived stem cell (ASC) expresses BAFF and its receptors, which may be associated with the differentiation process of ASC towards adipocyte.
Abstract: B cell activating factor (BAFF) and its receptors were regarded as elements of the immune system, regulating the fate of B cell. In recent years, these molecules were identified in a number of normal and pathological tissues, expanding their potential functions beyond the immune system. In this study, on the basis of molecular clone and prokaryotic expression of equine BAFF, we reported that equine adipose-derived stem cell (ASC) expressed BAFF and its receptors, which exhibited the increased expression during ASC adipogenic differentiation in vitro. Moreover, with the addition of recombinant protein His6-sBAFF, an increased differentiation of equine ASC towards adipocyte was detected. These results suggested that BAFF and its receptors might be associated with the differentiation process of ASC towards adipocyte in horse.
Copyright © 2013 Elsevier B.V. All rights reserved.
Publication Date: 2014-01-03 PubMed ID: 24389381DOI: 10.1016/j.intimp.2013.12.018Google Scholar: Lookup
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- Journal Article
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Summary
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This research study discovered that B cell activating factor (BAFF) and its receptors, typically associated with the immune system, are also expressed in horse adipose-derived stem cells. Furthermore, their presence appears to be associated with the differentiation of these stem cells into adipocytes, or fat cells, especially when the protein His6-sBAFF is introduced.
Immune System Molecules in Adipose-Derived Stem Cells
- The researchers found that B cell activating factor (BAFF) and its receptors, which have previously been known to play a role in the immune system, are also expressed in horse fat cells. More specifically, they are found in adipose-derived stem cells (ASC). This was a significant finding as these molecules had been typically associated only with the immune system.
Increasing ASC Adipogenic Differentiation
- The study observed an increase in the expression of BAFF and its receptors during the in-vitro differentiation of ASC. Differentiation is the process by which a less specialized cell becomes a more specialized cell type – in this case, ASC becoming fat cells or adipocytes.
Role of Recombinant Protein His6-sBAFF
- Upon introducing the recombinant protein His6-sBAFF, researchers detected an increased differentiation of ASC towards adipocyte, thus underlining the potential role of this protein in adipogenesis.
Implications of the Research
- This study suggests that BAFF and its receptors might be key players in the differentiation process of stem cells towards fat cells in horses. By unlocking a deeper understanding of this process, we could develop more efficacious treatments for various conditions affecting fat cells, such as obesity and metabolic disorders.
Summary
- This research expanded the understanding of BAFF and its receptors, showcasing their role beyond the immune system and pinpointing their involvement in adipogenesis. This discovery was demonstrated with the differentiation of equine adipose-derived stem cells with the involvement of the protein His6-sBAFF, underscoring the integral role of these molecules in the process of differentiation from stem cells to adipocytes.
Cite This Article
APA
Wu H, Han D, Jiang Z, Zhao D, Liu M, Xu X, Liu X, Yang L, Ji X, Wang M, Zhang S.
(2014).
Equine adipose-derived stem cell (ASC) expresses BAFF and its receptors, which may be associated with the differentiation process of ASC towards adipocyte.
Int Immunopharmacol, 18(2), 365-372.
https://doi.org/10.1016/j.intimp.2013.12.018 Publication
Researcher Affiliations
- Jiangsu Key Laboratory for Molecular & Medical Biotechnology, Life Science College, Nanjing Normal University, Nanjing 210023, PR China; Basic Medical College, Nanjing University of Chinese Medicine, Nanjing 210023, PR China; Jiangsu Key Laboratory for Aquatic Crustacean Diseases, Life Science College, Nanjing Normal University, Nanjing 210023, PR China; Jiangsu Key Laboratory for Supramolecular Medicinal Materials & Applications, Life Science College, Nanjing Normal University, Nanjing 210023, PR China.
- Jiangsu Key Laboratory for Molecular & Medical Biotechnology, Life Science College, Nanjing Normal University, Nanjing 210023, PR China; Jiangsu Key Laboratory for Aquatic Crustacean Diseases, Life Science College, Nanjing Normal University, Nanjing 210023, PR China; Jiangsu Key Laboratory for Supramolecular Medicinal Materials & Applications, Life Science College, Nanjing Normal University, Nanjing 210023, PR China.
- Jiangsu Key Laboratory for Molecular & Medical Biotechnology, Life Science College, Nanjing Normal University, Nanjing 210023, PR China; Jiangsu Key Laboratory for Aquatic Crustacean Diseases, Life Science College, Nanjing Normal University, Nanjing 210023, PR China; Jiangsu Key Laboratory for Supramolecular Medicinal Materials & Applications, Life Science College, Nanjing Normal University, Nanjing 210023, PR China.
- Jiangsu Key Laboratory for Molecular & Medical Biotechnology, Life Science College, Nanjing Normal University, Nanjing 210023, PR China; Jiangsu Key Laboratory for Aquatic Crustacean Diseases, Life Science College, Nanjing Normal University, Nanjing 210023, PR China; Jiangsu Key Laboratory for Supramolecular Medicinal Materials & Applications, Life Science College, Nanjing Normal University, Nanjing 210023, PR China.
- Jiangsu Key Laboratory for Molecular & Medical Biotechnology, Life Science College, Nanjing Normal University, Nanjing 210023, PR China; Jiangsu Key Laboratory for Aquatic Crustacean Diseases, Life Science College, Nanjing Normal University, Nanjing 210023, PR China; Jiangsu Key Laboratory for Supramolecular Medicinal Materials & Applications, Life Science College, Nanjing Normal University, Nanjing 210023, PR China.
- Jiangsu Key Laboratory for Molecular & Medical Biotechnology, Life Science College, Nanjing Normal University, Nanjing 210023, PR China; Jiangsu Key Laboratory for Aquatic Crustacean Diseases, Life Science College, Nanjing Normal University, Nanjing 210023, PR China; Jiangsu Key Laboratory for Supramolecular Medicinal Materials & Applications, Life Science College, Nanjing Normal University, Nanjing 210023, PR China.
- Jiangsu Key Laboratory for Molecular & Medical Biotechnology, Life Science College, Nanjing Normal University, Nanjing 210023, PR China; Jiangsu Key Laboratory for Aquatic Crustacean Diseases, Life Science College, Nanjing Normal University, Nanjing 210023, PR China; Jiangsu Key Laboratory for Supramolecular Medicinal Materials & Applications, Life Science College, Nanjing Normal University, Nanjing 210023, PR China.
- Jiangsu Key Laboratory for Molecular & Medical Biotechnology, Life Science College, Nanjing Normal University, Nanjing 210023, PR China; Jiangsu Key Laboratory for Aquatic Crustacean Diseases, Life Science College, Nanjing Normal University, Nanjing 210023, PR China; Jiangsu Key Laboratory for Supramolecular Medicinal Materials & Applications, Life Science College, Nanjing Normal University, Nanjing 210023, PR China.
- Jiangsu Key Laboratory for Molecular & Medical Biotechnology, Life Science College, Nanjing Normal University, Nanjing 210023, PR China; Jiangsu Key Laboratory for Aquatic Crustacean Diseases, Life Science College, Nanjing Normal University, Nanjing 210023, PR China; Jiangsu Key Laboratory for Supramolecular Medicinal Materials & Applications, Life Science College, Nanjing Normal University, Nanjing 210023, PR China.
- Basic Medical College, Nanjing University of Chinese Medicine, Nanjing 210023, PR China.
- Jiangsu Key Laboratory for Molecular & Medical Biotechnology, Life Science College, Nanjing Normal University, Nanjing 210023, PR China; Jiangsu Key Laboratory for Aquatic Crustacean Diseases, Life Science College, Nanjing Normal University, Nanjing 210023, PR China; Jiangsu Key Laboratory for Supramolecular Medicinal Materials & Applications, Life Science College, Nanjing Normal University, Nanjing 210023, PR China. Electronic address: shuangquan_z@yahoo.com.
MeSH Terms
- Adipocytes
- Adipose Tissue / cytology
- Amino Acid Sequence
- Animals
- B-Cell Activating Factor / chemistry
- B-Cell Activating Factor / genetics
- B-Cell Activating Factor / metabolism
- B-Cell Activation Factor Receptor / genetics
- B-Cell Activation Factor Receptor / metabolism
- Cell Differentiation / physiology
- Female
- Horses
- Molecular Sequence Data
- Protein Structure, Secondary
- Stem Cells / cytology
- Stem Cells / metabolism
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