Equine adipose tissue-derived extracellular vesicles enhance adipose mesenchymal stem cell survival ex vivo.
Abstract: Adipose tissue-derived extracellular vesicles (ATEVs) have garnered attention for their roles in intercellular communication and regulation. This study presents the first investigation of equine adipose tissue, with ATEV being extracted concurrently during the isolation of adipose-derived mesenchymal stem cells (ADSCs). Through CCK-8 cell proliferation assays and scratch migration assays, a significant promotional effect of ATEV on ADSCs was observed, which not only accelerated the proliferation rate of the stem cells but also enhanced their migratory capacity. The application of Trypan Blue exclusion and live-dead cell staining further demonstrated the positive effects of ATEV on maintaining the viability of ADSCs during ex vivo storage. These findings provide new insights into the potential applications of ATEV in stem cell therapy, tissue repair, and regenerative medicine.
Copyright © 2025 Elsevier Ltd. All rights reserved.
Publication Date: 2025-02-18 PubMed ID: 39978576DOI: 10.1016/j.tvjl.2025.106319Google Scholar: Lookup
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- Journal Article
Cite This Article
APA
Li D, Wang X, Yao J, Chen S.
(2025).
Equine adipose tissue-derived extracellular vesicles enhance adipose mesenchymal stem cell survival ex vivo.
Vet J, 310, 106319.
https://doi.org/10.1016/j.tvjl.2025.106319 Publication
Researcher Affiliations
- VetCell Pet Cell Research Center, Deja Lab, Foshan, Guangdong, China.
- College of Animal Science and Technology, Foshan University, Foshan, Guangdong, China.
- Service Center of Agriculture and Rural Affairs of Meixian District, Meizhou, Guangdong, China. Electronic address: yaojunyong2007@163.com.
- College of Animal Science and Technology, Foshan University, Foshan, Guangdong, China. Electronic address: yixu77@163.com.
MeSH Terms
- Animals
- Horses
- Mesenchymal Stem Cells / physiology
- Extracellular Vesicles / physiology
- Adipose Tissue / cytology
- Cell Survival
- Cell Proliferation
- Cell Movement
Conflict of Interest Statement
Declaration of Competing Interest The authors affirm that there are no recognized financial conflicts of interest or personal affiliations that may have had an impact on the research presented in this manuscript.
Citations
This article has been cited 2 times.- Dayalan H, Bupesh G, Kirubakaran D, Mathe D, Panigrahi J. Chloroquine as a potential anticancer agent for triple-negative breast cancer: effects on MDA-MB-231 cells.. Med Oncol 2025 Jun 8;42(7):245.
- Shi L, Khan MZ, Ullah A, Liang H, Geng M, Akhtar MF, Na J, Han Y, Wang C. Advancements in Stem Cell Applications for Livestock Research: A Review.. Vet Sci 2025 Apr 23;12(5).
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