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Home / Equine Research Bank / J Exp Med / Equine Antihapten Antibody. I. 7S Beta-2A- and 10S Gamma-1- ...
The Journal of experimental medicine1964; 120(4); 589-609; doi: 10.1084/jem.120.4.589

Equine Antihapten Antibody. I. 7S Beta-2A- and 10S Gamma-1- Globulin Components of Purified Anti-Beta-Lactoside Antibody.

Abstract: Anti-p-azophenyl-beta-lactoside (Lac) antibody has been isolated from equine antiserum by specific precipitation with Lac-HSA (human serum albumin) and Lac-Hy (hemocyanin). Antibody was separated from antigen by chromatography on DEAE cellulose and zone electrophoresis in solvents containing lactose. Six antigenically distinct immunoglobulins have been identified in purified equine anti-Lac antibody: 7S beta(2A)-globulin, 19S gamma-globulin, a 10S gamma(1) globulin, and three antigenically distinct 7S gamma-globulins. The specific hapten-binding activity of the 7S beta(2A)-antibody and of the 10S gamma(1)-antibody has been demonstrated by equilibrium dialysis and by analytical ultracentrifugation using a combination of schlieren and absorption optics. The 10S gamma(1)-globulin antibody may be representative of a hitherto undescribed class of immunoglobulins.
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Publication Date: 1964-10-01 PubMed ID: 14212122PubMed Central: PMC2137781DOI: 10.1084/jem.120.4.589Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article discusses the identification of six distinct immunoglobulins in purified equine antibodies against a specific type of antigen (Lac), providing evidence of an undiscovered class of immunoglobulins.

Research Methodology

  • The scientists started by isolating Anti-p-azophenyl-beta-lactoside (Lac) antibodies from horse antiserum. This was done using a procedure known as specific precipitation, using Lac-HSA (human serum albumin) and Lac-Hy (hemocyanin).
  • They then separated the antibodies from the antigens using DEAE cellulose chromatography and zone electrophoresis in lactose-containing solvents. These are standard techniques used in molecular biology to purify and separate proteins and other compounds.
  • The researchers then identified the distinct immunoglobulins in the purified antibody. Immunoglobulins are the proteins produced by the immune system to combat specific foreign invaders in the body.

Results and Findings

  • In total, the researchers identified six antigenically distinct immunoglobulins in the equine anti-Lac antibody: 7S beta(2A)-globulin, 19S gamma-globulin, 10S gamma(1) globulin, and three other unique 7S gamma-globulins.
  • They confirmed the specific hapten-binding activity of the 7S beta(2A)-antibody and the 10S gamma(1)-antibody through equilibrium dialysis and analytical ultracentrifugation methods. Hapten-binding activity refers to the antibody’s ability to recognise and bind to specific antigens.
  • The discovery of the 10S gamma(1)-globulin antibody may mark the identification of a previously undescribed class of immunoglobulins. This is a significant achievement as it expands and refines our understanding of the immune system.

Conclusion

  • The research contributes to our understanding of immunoglobulins and potentially opens up new avenues for investigating the immune system, disease resistance, and the development of vaccines. By identifying new forms of antibodies, this research could help us develop better strategies to combat diseases that current immune strategies struggle to manage.

Cite This Article

APA
ROCKEY JH, KLINMAN NR, KARUSH F. (1964). Equine Antihapten Antibody. I. 7S Beta-2A- and 10S Gamma-1- Globulin Components of Purified Anti-Beta-Lactoside Antibody. J Exp Med, 120(4), 589-609. https://doi.org/10.1084/jem.120.4.589

Publication

The Journal of experimental medicine
ISSN: 0022-1007
NlmUniqueID: 2985109R
Country: United States
Language: English
Volume: 120
Issue: 4
Pages: 589-609

Researcher Affiliations

ROCKEY, J H
    KLINMAN, N R
      KARUSH, F

        MeSH Terms

        • Animals
        • Antibodies
        • Antigens
        • Beta-Globulins
        • Chromatography
        • Glycosides
        • Haptens
        • Hemocyanins
        • Horses
        • Immune Sera
        • Immunoelectrophoresis
        • Immunoglobulin G
        • Immunoglobulin M
        • Lactose
        • Research
        • Serum Albumin
        • Ultracentrifugation
        • gamma-Globulins

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