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Home / Equine Research Bank / Virology / Equine arteritis virus is delivered to an acidic compartment...
Virology2008; 377(2); 248-254; doi: 10.1016/j.virol.2008.04.041

Equine arteritis virus is delivered to an acidic compartment of host cells via clathrin-dependent endocytosis.

Abstract: Equine arteritis virus (EAV) is an enveloped, positive-stranded RNA virus belonging to the family Arteriviridae. Infection by EAV requires the release of the viral genome by fusion with the respective target membrane of the host cell. We have investigated the entry pathway of EAV into Baby Hamster Kidney cells (BHK). Infection of cells assessed by the plaque reduction assay was strongly inhibited by substances which interfere with clathrin-dependent endocytosis and by lysosomotropic compounds. Furthermore, infection of BHK cells was suppressed when clathrin-dependent endocytosis was inhibited by expression of antisense RNA of the clathrin-heavy chain before infection. These results strongly suggest that EAV is taken up via clathrin-dependent endocytosis and is delivered to acidic endosomal compartments.
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Publication Date: 2008-06-24 PubMed ID: 18570963PubMed Central: PMC7103380DOI: 10.1016/j.virol.2008.04.041Google Scholar: Lookup
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  • Journal Article
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research indicates that the Equine arteritis virus (EAV) infects host cells through a process known as clathrin-dependent endocytosis, and is then delivered to the acidic compartments of those cells.

Overall Approach to the Research

  • The researchers studied how the Equine arteritis virus (EAV), a type of RNA virus, enters and infects Baby Hamster Kidney cells (BHK).
  • They specifically looked at whether the process of clathrin-dependent endocytosis, a specific procedure by which cells absorb materials from their surroundings, played a role in the virus’s infection process.

Experimentation and Findings

  • During the investigations, the researchers used substances that interfere with clathrin-dependent endocytosis and lysosomotropic compounds to examine how they affected the infection process of the EAV.
  • The infection of the BHK cells was found to be strongly inhibited—meaning the virus was less effective—when these substances were used.
  • In addition, when clathrin-dependent endocytosis was suppressed in the BHK cells through the expression of antisense RNA of the clathrin-heavy chain, the infection rate of EAV was also reduced.

Conclusions

  • Based on these findings, the researchers concluded that the EAV virus enters host cells through clathrin-dependent endocytosis.
  • Moreover, once the virus is inside the host cell, it’s delivered to acidic compartments within the cell, a step essential for the virus reproduction and further infection.

Implications

  • This study provides valuable insights into the mechanisms that EAV uses to infect cells, contributing to the broader understanding of how RNA viruses operate.
  • The findings can aid in the development of new antiviral strategies that target these specific processes to inhibit viral infection, particular focusing on blocking the clathrin-dependent endocytosis pathway.

Cite This Article

APA
Nitschke M, Korte T, Tielesch C, Ter-Avetisyan G, Tünnemann G, Cardoso MC, Veit M, Herrmann A. (2008). Equine arteritis virus is delivered to an acidic compartment of host cells via clathrin-dependent endocytosis. Virology, 377(2), 248-254. https://doi.org/10.1016/j.virol.2008.04.041

Publication

Virology
ISSN: 0042-6822
NlmUniqueID: 0110674
Country: United States
Language: English
Volume: 377
Issue: 2
Pages: 248-254

Researcher Affiliations

Nitschke, Matthias
  • Department of Biology/Biophysics, Humboldt University Berlin, Invalidenstr. 42, 10115 Berlin, Germany.
Korte, Thomas
    Tielesch, Claudia
      Ter-Avetisyan, Gohar
        Tünnemann, Gisela
          Cardoso, M Cristina
            Veit, Michael
              Herrmann, Andreas

                MeSH Terms

                • Animals
                • Arterivirus Infections / metabolism
                • Cell Line
                • Clathrin / metabolism
                • Cricetinae
                • Endocytosis / drug effects
                • Endocytosis / physiology
                • Endosomes / metabolism
                • Endosomes / virology
                • Equartevirus / genetics
                • Equartevirus / metabolism
                • Equartevirus / physiology

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