Equine beta-defensin-1: full-length cDNA sequence and tissue expression.
Abstract: beta-Defensins are cysteine-rich endogenously produced antimicrobial peptides that play an important role in innate immune defense. Although, previous investigations have identified beta-defensins in several mammalian species, no reports have identified equine beta-defensins. Using a strategy of database searching for expressed sequence tags (EST) we identified putative expression of equine beta-defensins in hepatic tissue. Based on this information, sequence specific primers were designed for the equine gene enabling the identification of the full-length cDNA sequence of equine beta-defensin-1. Comparative analyses showed that equine beta-defensin-1 has 46-52% amino-acid identity with other beta-defensins, sharing the greatest identity with porcine beta-defensin-1. Complete conservation of cysteine residues was maintained between the species evaluated, and RT-PCR analysis revealed diverse mRNA tissue expression for equine beta-defensin-1. These data extend the repertoire of equine antimicrobial peptides and expand our understanding of equine innate immunity.
Copyright 2004 Elsevier B.V.
Publication Date: 2004-04-29 PubMed ID: 15113660DOI: 10.1016/j.vetimm.2003.12.010Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research study discovers a new antimicrobial peptide, named Equine Beta-Defensin-1, present in horses. The study gets into the specifics of its gene structure, protein comparison with other species, and its presence in various tissues to contribute to our understanding of equine immunity.
Objective of Study
The central objective of the research was to enhance the understanding of the innate immune system of horses by identifying and analyzing a new antimicrobial peptide, named Equine Beta-Defensin-1. Previous studies have revealed the presence of beta-defensins, crucial components of the immune system, in several mammalian species, but not in horses. This study breaks new ground by locating equine beta-defensins in hepatic tissue.
- The primary motive was to search for the existence of beta-defensins in horses and document their characterizations.
- The team also compared the newly discovered equine beta-defensin-1 peptide to beta-defensin-1 found in other species.
Methodology
The team adopted a strategy of database searching for Expressed Sequence Tags (EST), leading them to identify the possible expression of equine beta-defensins in hepatic (liver) tissue.
- The initial step was to carry out a database search for expressed sequence tags (EST).
- After identifying the putative expression of equine beta-defensins, custom primers were designed to enable the determination of the full-length cDNA sequence of the equine beta-defensin-1 gene.
Findings
The complete cDNA sequence of equine beta-defensin-1 was determined, which is a significant finding. The study also established a comparison between the newly identified equine beta-defensin-1 and beta-defensins from other species.
- The equine beta-defensin-1 exhibited a 46-52% amino acid identity with beta-defensins in other species, with the closest identity to porcine (pig) beta-defensin-1.
- Cysteine residues were found to be completely conserved across all species evaluated.
- An RT-PCR analysis was carried out to check the tissue expression of the equine beta-defensin-1 mRNA, revealing its diverse presence in different types of tissues within horses.
Implications
Identification of equine beta-defensin-1
- Extend the spectrum of known equine antimicrobial peptides.
- These findings significantly contribute to the understanding of the innate immune defense mechanism in horses.
Cite This Article
APA
Davis EG, Sang Y, Blecha F.
(2004).
Equine beta-defensin-1: full-length cDNA sequence and tissue expression.
Vet Immunol Immunopathol, 99(1-2), 127-132.
https://doi.org/10.1016/j.vetimm.2003.12.010 Publication
Researcher Affiliations
- Department of Clinical Sciences, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, USA. edavis@vet.k-state.edu
MeSH Terms
- Amino Acid Sequence
- Animals
- Base Sequence
- DNA, Complementary / chemistry
- DNA, Complementary / genetics
- Expressed Sequence Tags
- Horses / immunology
- Liver / immunology
- Molecular Sequence Data
- Phylogeny
- Reverse Transcriptase Polymerase Chain Reaction / veterinary
- Sequence Alignment
- Sequence Analysis, DNA
- beta-Defensins / chemistry
- beta-Defensins / genetics
Citations
This article has been cited 7 times.- Pei L, Liu K, Wei W, Su H, Li F, Feng Y, Wang D, Li X, Hou Y, Cao G. Equus β-Defensin-1 Regulates Innate IMMUNE Response in S. aureus-Infected Mouse Monocyte Macrophage. Animals (Basel) 2022 Oct 27;12(21).
- Kumar R, Ali SA, Singh SK, Bhushan V, Mathur M, Jamwal S, Mohanty AK, Kaushik JK, Kumar S. Antimicrobial Peptides in Farm Animals: An Updated Review on Its Diversity, Function, Modes of Action and Therapeutic Prospects. Vet Sci 2020 Dec 18;7(4).
- Van Cleemput J, Poelaert KCK, Laval K, Vanderheijden N, Dhaenens M, Daled S, Boyen F, Pasmans F, Nauwynck HJ. An Alphaherpesvirus Exploits Antimicrobial β-Defensins To Initiate Respiratory Tract Infection. J Virol 2020 Mar 31;94(8).
- Chen H, Guo A, Lu Z, Tan S, Wang J, Gao J, Zhang S, Huang X, Zheng J, Xi J, Yi K. Agrobacterium tumefaciens-mediated transformation of a hevein-like gene into asparagus leads to stem wilt resistance. PLoS One 2019;14(10):e0223331.
- Ling YM, Chen JY, Guo L, Wang CY, Tan WT, Wen Q, Zhang SD, Deng GH, Lin Y, Kwok HF. β-defensin 1 expression in HCV infected liver/liver cancer: an important role in protecting HCV progression and liver cancer development. Sci Rep 2017 Oct 17;7(1):13404.
- Marth CD, Firestone SM, Glenton LY, Browning GF, Young ND, Krekeler N. Oestrous cycle-dependent equine uterine immune response to induced infectious endometritis. Vet Res 2016 Nov 8;47(1):110.
- Bruhn O, Grötzinger J, Cascorbi I, Jung S. Antimicrobial peptides and proteins of the horse--insights into a well-armed organism. Vet Res 2011 Sep 2;42(1):98.
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