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In vitro cellular & developmental biology. Animal2009; 46(2); 102-106; doi: 10.1007/s11626-009-9258-6

Equine bronchial epithelial cells differentiate into ciliated and mucus producing cells in vitro.

Abstract: We describe a method for creating differentiated equine bronchial epithelial cell cultures that can be used for in vitro studies including airway disease mechanisms and pathogen-host interactions. Our method is based on the culturing of human tracheobronchial epithelial cells at an air-liquid interface (ALI) in specific serum-free, hormone-supplemented medium. Bronchial epithelial cells are isolated and grown on T-Clear® insert membranes. Within 2 to 3 wk, cells differentiate into ciliated and mucus producing cells as demonstrated by confocal and electron microscopy. Furthermore, the demonstration of the two major gel-forming mucin species, Muc5ac and Muc5b, in our bronchial epithelial cell culture system validates this method for studies of respiratory tract disease of the horse.
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Publication Date: 2009-11-14 PubMed ID: 19915928DOI: 10.1007/s11626-009-9258-6Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • N.I.H.
  • Extramural
  • Research Support
  • Non-U.S. Gov't
  • Validation Study

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article presents a lab technique used to grow differentiated equine bronchial epithelial cells, which can aid in the study of airway diseases and how pathogens interact with hosts. These cells, which are isolated and grown at an air-liquid interface, differentiate into specialized ciliated and mucus producing cells, mimicking the types of cells found in respiratory diseases in horses.

Methodology

  • The researchers have developed a new method for creating differentiated equine bronchial epithelial cell cultures for in vitro studies. This can be used to study disease mechanisms and how pathogens interact with their hosts.
  • The method used by the researchers involves culturing these cells at an air-liquid interface (ALI) in a unique serum-free, hormone-supplemented medium. This is done using human tracheobronchial epithelial cells as a reference.
  • The bronchial epithelial cells are isolated and then grown on T-Clear® insert membranes. In this environment, the cells differentiate into two primary types: ciliated cells and mucus producing cells.
  • The differentiation of cells is observed and documented using both confocal and electron microscopy. They are able to verify the differentiation due to the unique attributes of the two kinds of cells.

Results

  • Through this method, within a span of 2 to 3 weeks, cells differentiate into ciliated and mucus producing types, demonstrating the effectiveness of the technique.
  • Along with this, the researchers found the presence of two key gel-forming mucin species, popularly known as Muc5ac and Muc5b, in their bronchial epithelial cell cultures. This is a critical discovery, as these two mucin species are common in many respiratory diseases and conditions.
  • The identification of Muc5ac and Muc5b therefore validates the potential of this method for studying respiratory tract diseases in horses, showing a direct correlation to the encoding genes involved in such diseases.

Conclusion

  • The method described in this paper provides a way to create ciliated and mucus producing cells from isolated bronchial epithelial cells, a significant step forward in the study of equine respiratory diseases.
  • The result adds a valuable tool for researchers studying the mechanisms of respiratory diseases in horses and the interactions between these diseases and various pathogens. Using this new technique, researchers can better understand and devise countermeasures for these diseases that affect a large population of horses.

Cite This Article

APA
Schwab UE, Fulcher ML, Randell SH, Flaminio MJ, Russell DG. (2009). Equine bronchial epithelial cells differentiate into ciliated and mucus producing cells in vitro. In Vitro Cell Dev Biol Anim, 46(2), 102-106. https://doi.org/10.1007/s11626-009-9258-6

Publication

In vitro cellular & developmental biology. Animal
ISSN: 1543-706X
NlmUniqueID: 9418515
Country: Germany
Language: English
Volume: 46
Issue: 2
Pages: 102-106

Researcher Affiliations

Schwab, Ute E
  • Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, NY, USA. ues3@cornell.edu
Fulcher, M Leslie
    Randell, Scott H
      Flaminio, M Julia
        Russell, David G

          MeSH Terms

          • Animals
          • Bronchi / cytology
          • Cell Culture Techniques
          • Cell Differentiation
          • Cells, Cultured
          • Cilia / ultrastructure
          • Epithelial Cells / metabolism
          • Epithelial Cells / ultrastructure
          • Horses
          • Microscopy, Electron, Transmission
          • Mucin 5AC / metabolism
          • Mucin-5B / metabolism

          Grant Funding

          • HLD55936 / PHS HHS

          References

          This article includes 18 references
          1. Proc Natl Acad Sci U S A. 2006 Nov 28;103(48):18131-6
            pubmed: 17116883
          2. Stain Technol. 1960 Nov;35:313-23
            pubmed: 13741297
          3. Infect Immun. 2002 Aug;70(8):4547-55
            pubmed: 12117967
          4. Am J Pathol. 1989 Mar;134(3):539-49
            pubmed: 2923184
          5. Arch Virol. 2001;146(11):2239-47
            pubmed: 11765925
          6. Am J Respir Cell Mol Biol. 1995 Mar;12(3):329-38
            pubmed: 7873199
          7. Equine Vet J. 2003 May;35(3):252-7
            pubmed: 12755427
          8. Am J Respir Cell Mol Biol. 1990 Feb;2(2):145-54
            pubmed: 2306371
          9. Equine Vet J. 2003 May;35(3):222-3
            pubmed: 12755421
          10. In Vitro Cell Dev Biol Anim. 1993 Jun;29(6):481-92
            pubmed: 27519750
          11. Eur Respir J. 1996 Sep;9(9):1913-22
            pubmed: 8880112
          12. In Vitro Cell Dev Biol Anim. 2008 Jul-Aug;44(7):179-84
            pubmed: 18594938
          13. Methods Mol Med. 2005;107:183-206
            pubmed: 15492373
          14. Am J Physiol Lung Cell Mol Physiol. 2007 Jun;292(6):L1396-404
            pubmed: 17293373
          15. In Vitro Cell Dev Biol Anim. 2004 Nov-Dec;40(10):303-11
            pubmed: 15780007
          16. Vet Immunol Immunopathol. 2008 Sep 15;125(1-2):8-17
            pubmed: 18597857
          17. Res Vet Sci. 1997 Jan-Feb;62(1):30-3
            pubmed: 9160421
          18. Am J Physiol Lung Cell Mol Physiol. 2009 Jan;296(1):L92-L100
            pubmed: 18931053

          Citations

          This article has been cited 9 times.
          1. Weldearegay YB, Brogaard L, Rautenschlein S, Meens J, Valentin-Weigand P, Schaaf D. Primary cell culture systems to investigate host-pathogen interactions in bacterial respiratory tract infections of livestock. Front Cell Infect Microbiol 2025;15:1565513.
            doi: 10.3389/fcimb.2025.1565513pubmed: 40415959google scholar: lookup
          2. Gautam LK, Harriott NC, Caceres AM, Ryan AL. Basic Science Perspective on Engineering and Modeling the Large Airways. Adv Exp Med Biol 2023;1413:73-106.
            doi: 10.1007/978-3-031-26625-6_5pubmed: 37195527google scholar: lookup
          3. Veerati PC, Mitchel JA, Reid AT, Knight DA, Bartlett NW, Park JA, Grainge CL. Airway mechanical compression: its role in asthma pathogenesis and progression. Eur Respir Rev 2020 Sep 30;29(157).
            doi: 10.1183/16000617.0123-2019pubmed: 32759373google scholar: lookup
          4. Cozens D, Sutherland E, Lauder M, Taylor G, Berry CC, Davies RL. Pathogenic Mannheimia haemolytica Invades Differentiated Bovine Airway Epithelial Cells. Infect Immun 2019 Jun;87(6).
            doi: 10.1128/IAI.00078-19pubmed: 30962401google scholar: lookup
          5. Van Cleemput J, Poelaert KCK, Laval K, Impens F, Van den Broeck W, Gevaert K, Nauwynck HJ. Pollens destroy respiratory epithelial cell anchors and drive alphaherpesvirus infection. Sci Rep 2019 Mar 18;9(1):4787.
            doi: 10.1038/s41598-019-41305-ypubmed: 30886217google scholar: lookup
          6. Cozens D, Sutherland E, Marchesi F, Taylor G, Berry CC, Davies RL. Temporal differentiation of bovine airway epithelial cells grown at an air-liquid interface. Sci Rep 2018 Oct 5;8(1):14893.
            doi: 10.1038/s41598-018-33180-wpubmed: 30291311google scholar: lookup
          7. Frellstedt L, Gosset P, Kervoaze G, Hans A, Desmet C, Pirottin D, Bureau F, Lekeux P, Art T. The innate immune response of equine bronchial epithelial cells is altered by training. Vet Res 2015 Jan 17;46(1):3.
            doi: 10.1186/s13567-014-0126-3pubmed: 25595212google scholar: lookup
          8. Abraham G, Zizzadoro C, Kacza J, Ellenberger C, Abs V, Franke J, Schoon HA, Seeger J, Tesfaigzi Y, Ungemach FR. Growth and differentiation of primary and passaged equine bronchial epithelial cells under conventional and air-liquid-interface culture conditions. BMC Vet Res 2011 Jun 7;7:26.
            doi: 10.1186/1746-6148-7-26pubmed: 21649893google scholar: lookup
          9. Rousseau K, Cardwell JM, Humphrey E, Newton R, Knight D, Clegg P, Thornton DJ. Muc5b is the major polymeric mucin in mucus from thoroughbred horses with and without airway mucus accumulation. PLoS One 2011;6(5):e19678.
            doi: 10.1371/journal.pone.0019678pubmed: 21602926google scholar: lookup
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