Equine CRISP-3: primary structure and expression in the male genital tract.
Abstract: Although originally described in the male rodent genital tract, cysteine-rich secretory proteins (CRISPs) are expressed in a variety of mammalian tissue and cell types. The proteins of the male genital tract have been observed associated to spermatozoa and are believed to play a role in mammalian fertilization. Here we describe the identification and primary structure of the first equine member of the CRISP family. Equine CRISP-3 is transcribed and expressed in the stallion salivary gland, in the ampulla and the seminal vesicle. It displays all 16 conserved cysteine residues and shows 82% homology to human and 78% to guinea pig CRISP-2 (AA1, TPX 1) and 77% to human CRISP-3. In contrast to other mammalia, in the horse CRISP-3 is synthesized in great amounts in the accessory sexual glands, ampulla and seminal vesicle, thus allowing the isolation of equine CRISP-3 in amounts suitable for biochemical, physiological and structural studies from stallion seminal plasma.
Publication Date: 1998-09-28 PubMed ID: 9748582DOI: 10.1016/s0167-4838(98)00122-8Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research highlights the identification and structure of CRISP-3, a cysteine-rich secretory protein, within the male equine, noting its high expression within parts of the reproductive system and its potential role in fertilization.
Study Objectives and Findings
- The study aimed to explore the identification and structure of the cysteine-rich secretory protein (CRISP-3) in horses.
- CRISP proteins, initially discovered in male rodent genital tracts, have been found in various mammalian tissues and cell types. These proteins play a role in mammalian fertilization, with observations being made of their association with spermatozoa.
- The equine CRISP-3 was found to be transcribed and expressed in significant quantities in the stallion’s salivary gland as well as other parts of the male reproductive system including the ampulla and seminal vesicle.
Comparison to Other Species and Implications
- Equine CRISP-3 displays all 16 conserved cysteine residues and exhibits a notable homology (likeness) to CRISP-2 in humans and guinea pigs (82% and 78% respectively) and also to human CRISP-3 (77%).
- Interestingly, unlike other mammals, horses synthesize CRISP-3 in large quantities in the ampulla and seminal vesicle, accessory sexual glands. This suggests a potential important role in equine fertility and for studying aspects of fertility at a biochemical, physiological and structural level.
- The research findings allow for the isolation of equine CRISP-3 in quantities suitable for further studies into its potential roles and applications. This could enable a deeper understanding of fertilization and fertility in horses and possibly in mammalian species more broadly.
Cite This Article
APA
Schambony A, Gentzel M, Wolfes H, Raida M, Neumann U, Töpfer-Petersen E.
(1998).
Equine CRISP-3: primary structure and expression in the male genital tract.
Biochim Biophys Acta, 1387(1-2), 206-216.
https://doi.org/10.1016/s0167-4838(98)00122-8 Publication
Researcher Affiliations
- Institut für Reproduktionsmedizin, Tierärztliche Hochschule, Bünteweg 15, D-30559 Hannover, Germany.
MeSH Terms
- Amino Acid Sequence
- Animals
- Base Sequence
- Cloning, Molecular
- Conserved Sequence / genetics
- Gene Expression Regulation / genetics
- Horses
- Male
- Mass Spectrometry
- Molecular Sequence Data
- Peptide Fragments / chemistry
- RNA, Messenger / metabolism
- Salivary Proteins and Peptides / chemistry
- Semen / chemistry
- Seminal Plasma Proteins
- Sequence Analysis, DNA
- Sequence Homology, Amino Acid
Citations
This article has been cited 7 times.- Gonzalez SN, Sulzyk V, Weigel Muñoz M, Cuasnicu PS. Cysteine-Rich Secretory Proteins (CRISP) are Key Players in Mammalian Fertilization and Fertility. Front Cell Dev Biol 2021;9:800351.
- Bubenickova F, Postlerova P, Simonik O, Sirohi J, Sichtar J. Effect of Seminal Plasma Protein Fractions on Stallion Sperm Cryopreservation. Int J Mol Sci 2020 Sep 3;21(17).
- Anifandis G, Messini C, Dafopoulos K, Sotiriou S, Messinis I. Molecular and cellular mechanisms of sperm-oocyte interactions opinions relative to in vitro fertilization (IVF). Int J Mol Sci 2014 Jul 22;15(7):12972-97.
- Koppers AJ, Reddy T, O'Bryan MK. The role of cysteine-rich secretory proteins in male fertility. Asian J Androl 2011 Jan;13(1):111-7.
- Roberts KP, Ensrud-Bowlin KM, Piehl LB, Parent KR, Bernhardt ML, Hamilton DW. Association of the protein D and protein E forms of rat CRISP1 with epididymal sperm. Biol Reprod 2008 Dec;79(6):1046-53.
- Olson JH, Xiang X, Ziegert T, Kittelson A, Rawls A, Bieber AL, Chandler DE. Allurin, a 21-kDa sperm chemoattractant from Xenopus egg jelly, is related to mammalian sperm-binding proteins. Proc Natl Acad Sci U S A 2001 Sep 25;98(20):11205-10.
- Bu Y, Wang P, Li S, Li L, Zhang S, Wei H. Semen Protein CRISP3 Promotes Reproductive Performance of Boars through Immunomodulation. Int J Mol Sci 2024 Feb 14;25(4).
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